| 5 Hills Fitness Limited | Arts & recreation | 2023 |
| A & W Irving Limited | Construction | 2021 |
| A T Fare (Hoghill) & Co. | | 1996 |
| ABI Ground Rents Limited | Real estate | 2011 |
| Auld Langholm Station Ltd | Real estate | 2021 |
| Borders Bespoke Ltd | Construction | 2024 |
| Borders Forestry Ltd | Agriculture & fishing | 2015 |
| Borders Group Ltd | Construction | 2025 |
| Bush of Ewes Campsite Ltd | Hospitality | 2026 |
| Castleholm Community Trust | Arts & recreation | 2023 |
| Castleview Catering Limited | Hospitality | 2025 |
| Charles Paisley & Sons Limited | Administrative & support | 2004 |
| Cobra Technical Ltd | Professional & scientific | 2019 |
| Creation Mill C.I.C. | Arts & recreation | 2021 |
| Crown Langholm Ltd | Hospitality | 2024 |
| Daniel Lacey Design and Furniture Limited | Manufacturing | 2003 |
| David Story (Glendivan) & Co | | 2000 |
| Derek Johnson Builders Ltd | Construction | 2023 |
| Edmond Castle Propco Limited | Professional & scientific | 2013 |
| Elle Catering Ltd | Hospitality | 2024 |
| Equorium Property Company Limited | Real estate | 1989 |
| Eskdale & Liddesdale Farming Limited | Agriculture & fishing | 2017 |
| Eskdale Hotel Ltd | Hospitality | 2012 |
| Ewart Wind Consultancy Ltd | Professional & scientific | 2012 |
| Ewes Water Caravan & Camping Park Company Limited | Hospitality | 1996 |
| EWM (2011) Limited | Professional & scientific | 2011 |
| EWM (Financial Services) Limited | Financial services | 1992 |
| EWM Investco Limited | Real estate | 2013 |
| Extra-Curricular Activity Connect (Exact) Ltd | Information & communication | 2024 |
| Fraxinus Excelsior Ltd | Information & communication | 2020 |
| Galeforce Investments Limited | Energy | 2012 |
| Gibson & Lumgair (Scotland) Limited | Extraterritorial | 1952 |
| Glenfirra of Langholm Ltd. | Other services | 2000 |
| Hoghill Trout Farm Limited | Agriculture & fishing | 2011 |
| J J Paterson & Co (Hopsrig) | | 1991 |
| James Ewart Racing Limited | Arts & recreation | 2004 |
| Jane Norman International Limited | Wholesale & retail | 2014 |
| Johnstone Roofing & Fencing Services Ltd | Construction | 2024 |
| KD Plumbing & Heating Borders Ltd | Construction | 2013 |
| Kodi 2016 Limited | Other services | 2016 |
| Langholm and District Day Centre for Older People | Health & social care | 2004 |
| Langholm Dyeing Co. Limited | Extraterritorial | 2010 |
| Langholm Playcare Ltd. | Health & social care | 1996 |
| Mark Seed Forest and Land Management Limited | Agriculture & fishing | 2012 |
| Messrs Wright Limited | Agriculture & fishing | 2008 |
| Muckle Toon Media Limited | Information & communication | 2017 |
| N. Cavers (Hoghill) & Co | | 2001 |
| Parneet Limited | Hospitality | 2022 |
| Paulproducts Limited | Wholesale & retail | 2024 |
| Pelosi's Ices Ltd | Wholesale & retail | 2018 |
| Proagencies Ltd | Wholesale & retail | 2013 |
| Proquip Group Limited | Professional & scientific | 2002 |
| Proquip IP Limited | Administrative & support | 2002 |
| Proquip Limited | Wholesale & retail | 2002 |
| R & T 2020 Limited | Real estate | 2019 |
| REIVER3D LLP | | 2016 |
| Ritual and Restore Ltd | Arts & recreation | 2024 |
| RME Veterinary Services Ltd | Professional & scientific | 2024 |
| Rokpa Developments Limited | Wholesale & retail | 1994 |
| S Richardson Contracting Limited | Agriculture & fishing | 2012 |
| THE Buccleuch Centre | Arts & recreation | 2005 |
| THE Cashmere Trail Limited | Real estate | 2017 |
| THE Crown Hotel (Langholm) Estates Limited | Real estate | 2020 |
| THE Edinburgh Woollen Mill (Group) Limited | Professional & scientific | 2006 |
| THE Eskdale Foundation | Administrative & support | 1996 |
| THE Gibson Group (Scotland) Limited | Professional & scientific | 1992 |
| THE Langholm Alliance | Other services | 2020 |
| THE Langholm Initiative Trading Company Ltd | Arts & recreation | 2021 |
| Tommy Ireland Ltd | Agriculture & fishing | 2016 |
| Upper Eskdale Development Group | Other services | 2006 |
| Wolf Roofs Ltd | Construction | 2026 |