| ROS Technology Ltd | Professional & scientific | 2013 |
| Rosa Audio Ltd | Information & communication | 2021 |
| Rosa Housing Limited | Real estate | 2018 |
| Rose (Lancaster) Ltd | Real estate | 2002 |
| Rose and Thistle Gardening Services Ltd | Administrative & support | 2012 |
| Rose of Sharon Lancaster Ltd | Wholesale & retail | 2025 |
| Rosebank PR and Communications Ltd | Professional & scientific | 2021 |
| Rosewood & Steel Ltd | Manufacturing | 2026 |
| Rosie Originals Ltd | Manufacturing | 2025 |
| Ross Farms(Lancs.)Limited | Other services | 1954 |
| Roth Developments Limited | Real estate | 2026 |
| Routledge Plumbing Ltd | Construction | 2012 |
| Roynon Dancewear Limited | Wholesale & retail | 2022 |
| RP Welding Ltd | Manufacturing | 2025 |
| RQ Properties Limited | Real estate | 2022 |
| RRA Incorporate Ltd | Professional & scientific | 2014 |
| Rrmews Property Ltd | Construction | 2021 |
| Rshcg Services Unlimited | Other services | 2024 |
| RSR Limited | Manufacturing | 1999 |
| RSR Trustee Limited | Real estate | 2007 |
| Rubik Niko Ltd | Wholesale & retail | 2011 |
| Ruby Star Holdings Limited | Financial services | 2024 |
| Ruddings International Limited | Wholesale & retail | 2024 |
| RUE Britannia Ltd | Manufacturing | 2015 |
| Run for Your Life CIC | Education | 2017 |
| Russell Regen Limited | Professional & scientific | 2023 |
| Rustlings Court Residents Company (NO. 2) Limited | Households | 1994 |
| Rustyration Limited | Manufacturing | 2018 |
| Ruth Wilkinson Consulting Ltd | Real estate | 2020 |
| RWB Site Services Ltd | Professional & scientific | 2014 |
| Ryeland Retail Ltd | Wholesale & retail | 2012 |
| S & W Leisure Lancaster Ltd | Hospitality | 2018 |
| S a H Fabrications Ltd | Professional & scientific | 2013 |
| S Clarke Construction Limited | Construction | 2014 |
| S Dickinson Limited | Construction | 2010 |
| S Graham Architects Limited | Professional & scientific | 2013 |
| S J Bargh Group Limited | Transport & logistics | 2016 |
| S J Papaslice Services Limited | Transport & logistics | 2015 |
| S P W ALL Access Limited | Mining & quarrying | 2017 |
| S Shah Lettings Ltd | Real estate | 2025 |
| S T Todd Ltd | Agriculture & fishing | 2003 |
| S. Rawes (Joinery) Limited | Construction | 2007 |
| S.J.Bargh Limited | Transport & logistics | 1954 |
| S.R. Brown Limited | Construction | 1998 |
| S2S Advisory Ltd | Professional & scientific | 2024 |
| SAF Lancaster Ltd | Hospitality | 2018 |
| SAF Locums Limited | Administrative & support | 2013 |
| Safe Child Africa | Extraterritorial | 2005 |
| Safe Hands Technical Ltd | Manufacturing | 2012 |
| SAFEBASE360 Ltd | Manufacturing | 2024 |
| Safenetics Limited | Professional & scientific | 2016 |
| Safety Rams Limited | Other services | 2018 |
| Safety Together Ltd | Professional & scientific | 2025 |
| Sagar 1977 Ltd | Health & social care | 2023 |
| Saha Law Limited | Professional & scientific | 2020 |
| Saks Consultancy Ltd | Information & communication | 2012 |
| Salty Spice Limited | Hospitality | 2025 |
| Samaf Electrical Limited | Information & communication | 2019 |
| Samantha Clay Limited | Arts & recreation | 2025 |
| Sambell Sports Camps Ltd | Education | 2025 |
| Same Day Express Limited | Transport & logistics | 1997 |
| Samkar Limited | Health & social care | 2013 |
| Sammy's Cosmetic Aesthetics Ltd | Other services | 2022 |
| Samskye Limited | Households | 2020 |
| Samson James Bluebell Limited | Real estate | 2022 |
| Samtal Limited | Professional & scientific | 2007 |
| Samuel's Court Management Company Ltd | Households | 2005 |
| Samuelson Wylie Associates Ltd | Professional & scientific | 2010 |
| Sanah's Caterers Limited | Hospitality | 2012 |
| Sanctum Inns Limited | Hospitality | 2026 |
| Sanders Recruitment Solutions Ltd | Administrative & support | 2024 |
| Sandgate Country Park Ltd | Hospitality | 2020 |
| Sandhurst Management Company (Arnside) Limited | Households | 2007 |
| Sandside (Cockerham) Limited | Households | 2009 |
| Sangana Limited | Information & communication | 2024 |
| Sarah Edney Speech and Language Therapy Limited | Health & social care | 2024 |
| Sarah Kettlewell Limited | Administrative & support | 2020 |
| Sarah Wagstaffe Ltd | Education | 2025 |
| SAS Barbers 1 Limited | Other services | 2026 |
| SAS Builders and Roofers Limited | Construction | 2016 |
| Savage Finance Limited | Professional & scientific | 2021 |
| Savage Pharma Ltd | Professional & scientific | 2026 |
| Savvy Studios Limited | Arts & recreation | 2018 |
| Sawme Ltd | Wholesale & retail | 2023 |
| Saymass Investments Ltd | Real estate | 2024 |
| SB Project Management Limited | Construction | 2025 |
| SBG Associates Limited | Professional & scientific | 2010 |
| Sbga Holdings Ltd | Professional & scientific | 2019 |
| SBH Group Limited | Hospitality | 2015 |
| SC Signs Ltd | Manufacturing | 2005 |
| Scale-Ability Ltd | Other services | 2018 |
| Scales Properties Limited | Real estate | 2026 |
| Scarcer Limited | Information & communication | 2009 |
| Scatterseed Limited | Financial services | 2025 |
| Scientists for Global Responsibility | Professional & scientific | 2022 |
| Sclpk Consulting Limited | Real estate | 2025 |
| Scorchedheads Ltd | Arts & recreation | 2025 |
| Scortec Systems Limited | Administrative & support | 2015 |
| Scotforth After School Club | Health & social care | 2010 |
| Scots Media Limited | Information & communication | 2020 |