| Lakeland Vehicle Solutions Limited | Financial services | 2010 |
| Lakeland Window Services Ltd | Construction | 2022 |
| Laker Legal Solicitors Ltd | Professional & scientific | 2013 |
| Lakes & Dales Asset Finance Limited | Extraterritorial | 2025 |
| Lakes & Dales Finance Limited | Extraterritorial | 2025 |
| Lakes Bistro Limited | Hospitality | 2008 |
| Lakes N Dales Ltd | Construction | 2014 |
| Lakes Physio Limited | Health & social care | 2023 |
| Lakeside Inns (Holdings) Limited | Financial services | 2023 |
| Lakeside Inns Limited | Hospitality | 1992 |
| Lamb's Plumbing and Heating Ltd | Construction | 2023 |
| Lancambe Court Residents Association (Lancaster) Limited | Households | 1984 |
| Lancashire Bulk Ltd | Transport & logistics | 2026 |
| Lancashire Cotton Corporation Ltd. | Manufacturing | 2018 |
| Lancashire Dent Solutions Limited | Wholesale & retail | 2010 |
| Lancashire GP Ltd | Public administration | 2026 |
| Lancashire Mechanical and Welding Services Limited | Manufacturing | 2026 |
| Lancashire MSK & Spine Solutions Limited | Health & social care | 2024 |
| Lancashire Online Limited | Professional & scientific | 2022 |
| Lancashire Roofing & Building Ltd | Construction | 2020 |
| Lancaster & District Homeless Action Service Limited | Health & social care | 1999 |
| Lancaster & Morecambe Chamber of Commerce | Other services | 1897 |
| Lancaster Aerospace Ltd | Manufacturing | 2026 |
| Lancaster and District Festival Limited | Arts & recreation | 1980 |
| Lancaster and Morecambe Makers | Other services | 2014 |
| Lancaster Art Fair CIC | Administrative & support | 2024 |
| Lancaster Bathrooms Limited | Construction | 2020 |
| Lancaster BID Ltd | Other services | 2016 |
| Lancaster Boxing CIC | Arts & recreation | 2024 |
| Lancaster Brewery Company Limited | Manufacturing | 2005 |
| Lancaster Brewery Group Limited | Manufacturing | 2023 |
| Lancaster Brewery Inns Ltd | Hospitality | 2003 |
| Lancaster Building Contractors Limited | Construction | 2009 |
| Lancaster Canal Regeneration Partnership CIC | Transport & logistics | 2022 |
| Lancaster Castle Holdings Ltd | Hospitality | 2019 |
| Lancaster City Transport Ltd | Transport & logistics | 2008 |
| Lancaster Cleaning Company Ltd | Administrative & support | 2020 |
| Lancaster Coatings Ltd | Construction | 2020 |
| Lancaster Cohousing Company Ltd. | Households | 2006 |
| Lancaster Community Music Trust | Arts & recreation | 1994 |
| Lancaster Concrete Limited | Extraterritorial | 2018 |
| Lancaster Design Solutions Ltd | Professional & scientific | 2013 |
| Lancaster Detailing Ltd | Professional & scientific | 2023 |
| Lancaster Distillery & Spirits Company Ltd | Hospitality | 2009 |
| Lancaster District Community and Voluntary Solutions | Other services | 2008 |
| Lancaster District Magazine Ltd | Information & communication | 2014 |
| Lancaster Driveways Limited | Administrative & support | 2016 |
| Lancaster Fencing & Forrestry Ltd | Construction | 2023 |
| Lancaster Fencing Limited | Construction | 2018 |
| Lancaster Fibre Technology Ltd | Manufacturing | 2021 |
| Lancaster Film Initiative CIC | Education | 2023 |
| Lancaster Girls' Grammar School | Education | 2010 |
| Lancaster Glass Fibre Limited | Manufacturing | 2021 |
| Lancaster Golf Club Ltd | Arts & recreation | 1932 |
| Lancaster Group Holdings Limited | Information & communication | 2018 |
| Lancaster Haematology Ltd | Health & social care | 2025 |
| Lancaster Helium Ltd | Manufacturing | 2016 |
| Lancaster Independent School for Alternative Learning Ltd | Education | 1997 |
| Lancaster Instruments Ltd | Manufacturing | 2023 |
| Lancaster Insulation Limited | Construction | 2012 |
| Lancaster Jazz Festival | Arts & recreation | 2012 |
| Lancaster Living Limited | Real estate | 2025 |
| Lancaster Local Barber Ltd | Other services | 2024 |
| Lancaster Local Market Ltd | Wholesale & retail | 2025 |
| Lancaster Lodgings Limited | Real estate | 2010 |
| Lancaster LP Holdings Ltd | Real estate | 2021 |
| Lancaster Masonic Hall Limited | Other services | 1957 |
| Lancaster Meat Company (NW) Ltd | Wholesale & retail | 2015 |
| Lancaster Medical Ambulance Service Limited | Health & social care | 2022 |
| Lancaster Medical Group Limited | Health & social care | 2017 |
| Lancaster Medical Limited | Health & social care | 2022 |
| Lancaster Music Festival CIC | Arts & recreation | 2018 |
| Lancaster OFF Road Ltd | Arts & recreation | 2006 |
| Lancaster on Ice Ltd | Hospitality | 2018 |
| Lancaster One Management Company Limited | Households | 2003 |
| Lancaster Pizza House Ltd | Hospitality | 2024 |
| Lancaster Plant Engineers Ltd | Manufacturing | 2026 |
| Lancaster Pottery Studio Limited | Manufacturing | 2022 |
| Lancaster Pro Builders Ltd | Construction | 2024 |
| Lancaster Property Management Ltd | Real estate | 2025 |
| Lancaster Races Limited | Arts & recreation | 2015 |
| Lancaster Royal Grammar School | Education | 2010 |
| Lancaster Rubber and Industrial Supplies Limited | Manufacturing | 1999 |
| Lancaster Rubber Properties Ltd. | Real estate | 2017 |
| Lancaster Scaffolding Limited | Construction | 2019 |
| Lancaster Signs Limited | Professional & scientific | 2016 |
| Lancaster Smile Limited | Health & social care | 2021 |
| Lancaster SR Ltd | Hospitality | 2025 |
| Lancaster Stores LP Ltd | Wholesale & retail | 2021 |
| Lancaster Student Lettings Limited | Real estate | 2017 |
| Lancaster TAP & Table Ltd | Hospitality | 2025 |
| Lancaster Trade Ltd | Wholesale & retail | 2025 |
| Lancaster Trailers Ltd | Construction | 2023 |
| Lancaster University Business Enterprises Ltd | Professional & scientific | 1969 |
| Lancaster University Consultancy Services Limited | Professional & scientific | 1970 |
| Lancaster University Developments Limited | Professional & scientific | 1993 |
| Lancaster University Energy Services Ltd | Energy | 1989 |
| Lancaster University Enterprises Ltd | Professional & scientific | 1994 |
| Lancaster University Network Services Limited | Information & communication | 2001 |
| Lancaster University Students' Union | Education | 2016 |