| Little Moss Farm Limited | Agriculture & fishing | 2018 |
| Littlenessies Ltd | Arts & recreation | 2011 |
| LJW Joiners Limited | Construction | 2022 |
| LNK Foods Ltd | Hospitality | 2024 |
| Loads Straps & Slings Limited | Manufacturing | 2007 |
| Loam Maintenance Limited | Construction | 2023 |
| Loaningdale School Company (the) | Health & social care | 1962 |
| Local Letting Agency Ltd | Real estate | 2020 |
| Loch TAY Services Ltd | Information & communication | 2022 |
| Loch Van Hire Ltd | Administrative & support | 2024 |
| Lochstone Property Investments Ltd | Real estate | 2025 |
| Lock Global Recruitment Ltd | Administrative & support | 2025 |
| Logan Vehicle Services Ltd | Wholesale & retail | 2022 |
| Loia Limited | Hospitality | 2010 |
| Lorical Consulting Limited | Professional & scientific | 2013 |
| Lothian Laser Clinic Ltd | Other services | 2020 |
| Lotus Foods Ltd | Real estate | 2016 |
| Lotus Steels Ltd | Wholesale & retail | 2013 |
| LS Plumbing and Heating (Bothwell) Limited | Construction | 2024 |
| LSB Industrial Solutions Ltd | Professional & scientific | 2016 |
| LSM Groundworks (Scotland) Limited | Construction | 2024 |
| Lucky's Praetorium Limited | Other services | 2023 |
| Lumavox Ltd | Manufacturing | 2024 |
| Lumogence Limited | Information & communication | 2016 |
| Lunar Holdings & Investments Ltd | Financial services | 2024 |
| Lunar Stack Ltd | Information & communication | 2020 |
| Luxe and Evolve Clinic Ltd | Other services | 2025 |
| Lynsey Mckinlay Ltd | Health & social care | 2025 |
| M & J Solutions Ltd | Professional & scientific | 2018 |
| M & W Bar Ltd | Hospitality | 2015 |
| M Greenan Consulting Limited | Administrative & support | 2022 |
| M&J Nosebags Ltd | Hospitality | 2023 |
| M&M Electrical Services (Scotland) Limited | Construction | 2024 |
| M&N Fiber Connect Ltd | Construction | 2023 |
| M.A.A Care Alliances Ltd | Health & social care | 2025 |
| M.C.A.V. Engineering & Inspection Services Ltd | Manufacturing | 2024 |
| M.G. Cars Limited | Wholesale & retail | 2006 |
| M74 Self Storage Limited | Transport & logistics | 2024 |
| MAA SVS Ltd | Administrative & support | 2019 |
| Maam Co. Ltd | Health & social care | 2025 |
| Maam Holdings Co. Ltd | Financial services | 2025 |
| Macaviso Investments Limited | Real estate | 2021 |
| Macfabmetal Limited | Manufacturing | 2013 |
| Mackinnon Farming Ltd | Agriculture & fishing | 2018 |
| Madras Cottage Ltd | Hospitality | 2010 |
| Mainstreet Ltd | Real estate | 2008 |
| Maira Investment Property Ltd | Real estate | 2023 |
| Make the Connection C.I.C. | Health & social care | 2015 |
| Maktub Morocco Desert Ltd | Administrative & support | 2026 |
| Mall Music Broadcasting Ltd. | Information & communication | 2003 |
| Mallow Dream Ltd | Manufacturing | 2025 |
| Maple Engineering Limited | Manufacturing | 2025 |
| Marc Brew Company | Arts & recreation | 2013 |
| Marchmont Healthcare Limited | Wholesale & retail | 2025 |
| Marek Construction Ltd | Construction | 2019 |
| Mariyana Slavcheva Limited | Administrative & support | 2022 |
| Marketing Services (Scotland) Limited | Real estate | 2002 |
| Martin Precision EOT Ltd | Financial services | 2023 |
| Martin Precision Ltd | Manufacturing | 1993 |
| Mason K Electrical Solutions Ltd | Construction | 2026 |
| Masonry King Construction Ltd | Construction | 2017 |
| Master Chief Limited | Hospitality | 2025 |
| Matt Bryce Training Ltd | Education | 2025 |
| Maverick Realty Ltd | Real estate | 2024 |
| Maywood Reproductions Limited | Wholesale & retail | 1985 |
| MB Roofing & Building Specialists Ltd | Construction | 2026 |
| MC Rock Ltd | Construction | 2021 |
| Mccourt Construction Ltd | Construction | 2023 |
| Mccourt Roofing Ltd | Construction | 2011 |
| Mcdsolarshine Ltd | Administrative & support | 2025 |
| Mcewan & SON Ltd | Hospitality | 2024 |
| Mcivor Property Company Limited | Real estate | 2025 |
| Mcjd Limited | Hospitality | 2021 |
| Mckay's Multi Trade Limited | Construction | 2026 |
| Mcrae Media Ltd | Professional & scientific | 2023 |
| MDC Power Ltd | Manufacturing | 2025 |
| Media by Mida Limited | Construction | 2025 |
| Medwyn Livestock Services Limited | Agriculture & fishing | 2014 |
| Medwyn Vets Limited | Professional & scientific | 2018 |
| Menunomics Ltd | Professional & scientific | 2026 |
| Mero Ltd | Health & social care | 2018 |
| Mfsc Engineering Ltd | Professional & scientific | 2025 |
| Mgmt Rosa Renewable Energy Advisory Ltd | Professional & scientific | 2026 |
| MH14 Trading Ltd | Wholesale & retail | 2026 |
| MIA Catering Ltd | Hospitality | 2023 |
| Millar & Mcgowan Limited | Wholesale & retail | 1973 |
| Millyard Peoples Ltd | Administrative & support | 2021 |
| Minerva Livingston Ltd | Hospitality | 2026 |
| Minsu Ltd | Wholesale & retail | 2025 |
| Mira Clad Ltd | Construction | 2018 |
| Mira Property Ltd | Real estate | 2017 |
| Miss Mcsweep Ltd | Administrative & support | 2026 |
| MJB Landscaping Ltd | Construction | 2025 |
| ML11 Distribution Ltd | Wholesale & retail | 2018 |
| MLM Consultancy & Training Services Ltd | Education | 2023 |
| MM Barbers Lanark Ltd | Other services | 2020 |
| MN Fiber Connect Ltd | Construction | 2024 |
| Mobile Welding Services Ltd. | Construction | 2004 |
| Modus Planning Ltd | Professional & scientific | 2025 |
| Moss Energy Limited | Agriculture & fishing | 2020 |