| Heatherdale Pest Control Limited | Administrative & support | 2026 |
| Heli Air Scotland Limited | Transport & logistics | 2013 |
| Henderson Lifting Services Ltd | Construction | 2012 |
| High Impact Training & Consulting Ltd | Information & communication | 2023 |
| Highpass IT & Consultancy Limited | Information & communication | 2023 |
| Highsparks T.C.S. Limited | Construction | 2008 |
| Hijh Power Solutions Ltd | Education | 2026 |
| Hill Park Autos Ltd | Wholesale & retail | 2026 |
| Hilland Mcnulty Limited | Professional & scientific | 2010 |
| Hmgb Development Ltd | Construction | 2019 |
| HMR Legacy Ltd | Real estate | 2017 |
| Hodge Heavy Haulage Limited | Transport & logistics | 1999 |
| Hollingsworth Engineering Ltd | Manufacturing | 2020 |
| Homan Global Industries Ltd | Construction | 2020 |
| Home Pool Tables Limited | Wholesale & retail | 2024 |
| Hometown Foundation | Professional & scientific | 2009 |
| Honeyman Homes Ltd | Real estate | 2025 |
| Hook Marine Limited | Professional & scientific | 1999 |
| Hope Street Investments Limited | Real estate | 1998 |
| Hopemangreen (East) Limited | Construction | 2001 |
| Hopetoun Hair Ltd | Other services | 2016 |
| Horse 2 Home Ltd | Professional & scientific | 2020 |
| Howeburn Livestock Services L Ltd | Agriculture & fishing | 2023 |
| HR Contractors (Scotland) Ltd | Construction | 2025 |
| HS Residential Group Ltd | Households | 2023 |
| Humanity Enterprises Ltd | Information & communication | 2022 |
| Husabost Holding Ltd | Health & social care | 2021 |
| Hyndford Estates Limited | Construction | 2003 |
| Hyndford Plant Limited | Construction | 1972 |
| Hynds Consulting Ltd | Professional & scientific | 2024 |
| Hyperbaric Oxygen Treatment (Hbot) Ltd | Health & social care | 2025 |
| I a Stewart & Co Limited | Professional & scientific | 2013 |
| IAN Cuthbert Plumbing and Heating Ltd. | Construction | 2017 |
| Iann Limited | Other services | 2025 |
| ICW Accountancy Ltd | Professional & scientific | 2010 |
| Ideal GAS Ltd | Manufacturing | 2015 |
| Ignite Scotland Heating Limited | Construction | 2008 |
| Impact Shopfitting Scotland Ltd | Financial services | 2021 |
| Incorporated Blind Solutions Ltd | Construction | 2019 |
| Incr-Edibles CBD Products Ltd | Wholesale & retail | 2023 |
| Indieng Ltd | Professional & scientific | 2021 |
| Indigo Sapphire Ltd | Information & communication | 2023 |
| Industrial Inspection Services Ltd. | Professional & scientific | 2006 |
| Infinite Bathrooms Ltd | Construction | 2026 |
| Infinity Estate & Letting Ltd | Real estate | 2022 |
| Infinity Wind Limited | Construction | 2025 |
| Inheritance Legal Executors Limited | Professional & scientific | 2023 |
| Inheritance Legal Wills Limited | Professional & scientific | 2021 |
| Intra Events Limited | Arts & recreation | 2025 |
| Inverliever Hydro Limited | Energy | 2007 |
| Iris Financial Solutions Limited | Financial services | 2026 |
| Isaiah Stewart | | 1980 |
| It's Personal Ltd | Wholesale & retail | 2024 |
| Ivalon Limited | Construction | 2025 |
| J Gibson Agri Ltd | Construction | 2021 |
| J M Mcneish Ceramic Tile Services Limited | Construction | 2011 |
| J M Shearer Limited | Agriculture & fishing | 2015 |
| J T Plant Limited | Administrative & support | 2005 |
| J&A Struthers Limited | Agriculture & fishing | 2024 |
| J&J Logistical Solutions Ltd | Transport & logistics | 2023 |
| J-Tech Commercial & Agri Services Limited | Wholesale & retail | 2024 |
| J.B.R. Plant & Truck Services Limited | Manufacturing | 2015 |
| J.M.T. Manufacturing Limited | Wholesale & retail | 1986 |
| Jacg Holdings Limited | Other services | 2025 |
| Jack's of Lanark Ltd. | Wholesale & retail | 2003 |
| Jacks Lanark Ltd. | Wholesale & retail | 2003 |
| James Forrest & Company | | 1985 |
| James R Craig Limited | Professional & scientific | 2018 |
| James Steele Ltd | Energy | 2012 |
| Jamie Doak Joinery Limited | Construction | 2019 |
| JAN Neilson Limited | Administrative & support | 2016 |
| JAN Rooney Hair Design Limited | Other services | 2012 |
| Japaco Limited | Information & communication | 2019 |
| Jappacakes Ltd | Manufacturing | 2025 |
| Jarl Consulting Ltd | Professional & scientific | 2012 |
| Jarvie S Limited | Wholesale & retail | 2016 |
| JB Roughcasting Limited | Construction | 2024 |
| Jbarrie Ltd | Construction | 2026 |
| JBG Homes Limited | Real estate | 2026 |
| Jcgprojects Ltd | Arts & recreation | 2023 |
| JDC Ecology Limited | Professional & scientific | 2002 |
| JDS Property Developers Ltd | Construction | 2018 |
| Jerviswood Project Management Limited | Construction | 2014 |
| Jerwin Coatings Ltd | Manufacturing | 2000 |
| Jfgm Limited | Administrative & support | 2020 |
| JHP Aggregates and Decorative Stone Limited | Wholesale & retail | 2023 |
| JHP Transport (Holdings) Limited | Transport & logistics | 2018 |
| JHP Transport (Lanark) Limited | Transport & logistics | 2013 |
| JJK Roofing Limited | Construction | 2026 |
| JKT Joiners Ltd | Construction | 2018 |
| Jkub Ltd | Professional & scientific | 2023 |
| JM Roofing & Rough Casting Ltd Ltd | Construction | 2026 |
| John Gold Floristry Limited | Wholesale & retail | 2021 |
| John Hinks Research Ltd | Other services | 2016 |
| John Smith Slater & Plasterer Limited | Construction | 2006 |
| John Weir Joiners Limited | Construction | 2012 |
| Jorax Construction Ltd | Construction | 2025 |
| Jordex Limited | Construction | 2025 |
| Joulecraft Ltd | Construction | 2026 |
| JP Mitchell Meats Ltd | Wholesale & retail | 2025 |