| E&C Surfacing Ltd | Construction | 2026 |
| East Mohr Rare Breeds Ltd | Agriculture & fishing | 2023 |
| Eastford Excavations Holdings Limited | Construction | 2023 |
| Eastford Excavations Limited | Construction | 2007 |
| Eavallee Ltd | Wholesale & retail | 2024 |
| EBI Restaurants Limited | Hospitality | 2010 |
| Ecoclean Scotland Ltd | Administrative & support | 2025 |
| Ecosse Glamping Ltd | Hospitality | 2022 |
| Edge Lanark Ltd | Other services | 2020 |
| Edinburgh Building Safety Organisation Limited | Other services | 1962 |
| Edinburgh First AID Ltd | Other services | 2025 |
| Edinburgh Psychiatry Ltd | Health & social care | 2018 |
| Edinburgh Relocations Ltd | Real estate | 2026 |
| Edinburgh Training Ltd | Health & social care | 2025 |
| Education Resource Ltd. | Education | 2015 |
| Eitten Ltd | Energy | 2012 |
| EK Agriplant Limited | Manufacturing | 2026 |
| Ekotransport Limited | Transport & logistics | 2014 |
| Elaina Care Home Limited | Health & social care | 2003 |
| Electriciansmate Ltd | Information & communication | 2025 |
| Elisabeth Johnstone Ltd | Construction | 2025 |
| Elite Comfort Ltd | Wholesale & retail | 2026 |
| Elite Hygiene & Maintenance Services Ltd. | Administrative & support | 2013 |
| Ellis Vale Electrical Limited | Construction | 2022 |
| ELO Market Limited | Wholesale & retail | 2025 |
| Elsrickle Ltd | Construction | 2013 |
| Elvarix UK Ltd | Wholesale & retail | 2026 |
| EM Cubed Ltd | Professional & scientific | 2005 |
| EMC Property Investments Ltd | Real estate | 2022 |
| Empirical Group Holdings Limited | Financial services | 2025 |
| Empirical Group Ltd | Construction | 2021 |
| Enchanted Aesthetics by T&T Ltd | Other services | 2024 |
| Engiclean Ltd | Wholesale & retail | 2025 |
| Engineering Survey and Design Limited | Professional & scientific | 2020 |
| Enlite Electrical Limited | Construction | 2021 |
| Entrepreneurial Support Services UK Ltd | Professional & scientific | 2025 |
| EP Collective Ltd | Health & social care | 2021 |
| EPS Properties Maintenance Ltd | Construction | 2017 |
| Equi-Elite Ltd | Wholesale & retail | 2017 |
| Equine Rehabilitation Centre Limited | Agriculture & fishing | 2021 |
| Equipt Barbering Limited | Other services | 2026 |
| Equus Liberty Ltd | Agriculture & fishing | 2026 |
| Ernelian Limited | Wholesale & retail | 2025 |
| Esper Motion Limited | Professional & scientific | 2022 |
| Euroboat Ltd | Transport & logistics | 2024 |
| Everything IS Copy Limited | Arts & recreation | 2021 |
| Excel Signs (Scotland) Limited | Professional & scientific | 2006 |
| Executive Benefit Services (U.K.) Limited | Financial services | 1973 |
| Expert Psychiatric Assessments Limited | Health & social care | 2013 |
| Expert Technical Services Limited | Information & communication | 2009 |
| FAIR8 Limited | Wholesale & retail | 2026 |
| Fairfield Marketing & Promotions L.P. | | 2012 |
| Fairfields ACC Limited | Wholesale & retail | 2014 |
| Fairfields Property Ltd | Real estate | 2024 |
| Farm Field Fresh Ltd. | Transport & logistics | 2009 |
| Fastroi Ltd | Information & communication | 2018 |
| Fbjrt Limited | Construction | 2018 |
| Ferradex Limited | Professional & scientific | 2025 |
| FF Equi Arena's Limited | Agriculture & fishing | 2016 |
| Field Management Logistics Ltd | Administrative & support | 2020 |
| Finewood Construction Ltd | Construction | 2026 |
| Fintech Futures Ltd | Professional & scientific | 2023 |
| Fiona Binning Developments Limited | Real estate | 2014 |
| Firebloome Limited | Manufacturing | 2010 |
| Fired in Scotland Ltd | Construction | 2023 |
| Flowers by Lavender Lane Limited | Wholesale & retail | 2007 |
| Focused Frog Ltd | Professional & scientific | 2026 |
| Folkerton Mill Weddings Ltd | Hospitality | 2019 |
| Foodfare Supermarket Ltd | Wholesale & retail | 2025 |
| FOODKOURT5 Ltd | Hospitality | 2023 |
| Forth and District Initiative | Extraterritorial | 2013 |
| FOX for Fitness Limited | Arts & recreation | 2026 |
| Francos Mossdale Limited | Real estate | 2012 |
| Frank Swords & Sons Limited | Construction | 2006 |
| Fraser Collins Consulting Ltd | Professional & scientific | 2025 |
| Fraser Dunlop Limited | Transport & logistics | 1999 |
| Fraser Lamb Ltd | Professional & scientific | 2018 |
| FRB Holdings Limited | Other services | 2025 |
| Freyah Ashton Limited | Real estate | 2025 |
| From the Soul LLP | | 2023 |
| FSP Agripets Ltd | Wholesale & retail | 2025 |
| FSP Group Limited | Financial services | 2024 |
| FSP Logistics Ltd | Transport & logistics | 2024 |
| FUN After 3 Club Ltd | Education | 2020 |
| Futureideas Limited | Wholesale & retail | 2021 |
| Futureidea’S Ltd | Wholesale & retail | 2020 |
| G & S Healthcare Ltd | Wholesale & retail | 2016 |
| G a Aitken Builders Limited | Construction | 2025 |
| G Ingram and Company Limited | Professional & scientific | 1999 |
| G Law Properties Limited | Real estate | 2025 |
| G N Tait Ltd | Construction | 2014 |
| G S Barbers Ltd | Other services | 2016 |
| G S G Property Developments Limited | Construction | 2005 |
| G-Brand Secure Systems Limited | Administrative & support | 2026 |
| G.I Services Ltd | Administrative & support | 2023 |
| Gallacher Contracts Limited | Administrative & support | 2018 |
| Gallagher Scott Ltd | Information & communication | 2019 |
| Gardiners of Scotland Limited | Manufacturing | 2000 |
| Garrion Garage Transport Ltd | Transport & logistics | 2013 |
| Gateway Consultancy Services Limited | Professional & scientific | 2011 |