| Sykes Propery Ventures Limited | Construction | 2026 |
| Sylvn Ltd | Information & communication | 2019 |
| Synaptein Limited | Other services | 2012 |
| Syntar Limited | Information & communication | 2026 |
| Synthetic Studios Ltd | Information & communication | 2021 |
| T Black Holdings Ltd | Financial services | 2025 |
| TAI Builders Limited | Construction | 2005 |
| Tait HR Ltd | Administrative & support | 2019 |
| Tandoor Mahal Limited | Hospitality | 2025 |
| Tapetex Ltd | Construction | 2018 |
| Tartan Engineering Services Ltd | Professional & scientific | 2025 |
| Taylor Yuill Transport Ltd | Transport & logistics | 2020 |
| Taylor'D Transport Ltd | Transport & logistics | 2020 |
| TC Stewart and Sons Limited | Manufacturing | 2021 |
| Tclc OLE Ltd | Professional & scientific | 2018 |
| Tea Time Lanarkshire Limited | Wholesale & retail | 2022 |
| Teal Group (Scotland) Limited | Construction | 2023 |
| Technogroom Limited | Other services | 2008 |
| Technology Helpdesk Ltd | Information & communication | 2024 |
| Tefs Ltd | Wholesale & retail | 2017 |
| Tennant Transport (Forth) Limited | Transport & logistics | 2003 |
| Tennant Transport Limited | Transport & logistics | 1974 |
| That Notion Ltd | Administrative & support | 2022 |
| Thato FOX Collective Ltd | Manufacturing | 2025 |
| THE Adventures of Isla and Harris Limited | Wholesale & retail | 2025 |
| THE Apple PIE Carnwath Ltd | Wholesale & retail | 2012 |
| THE Applebank Inn 1715 Limited | Hospitality | 2025 |
| THE Beauty Room (Abbeygreen) Ltd | Other services | 2006 |
| THE Beauty Spot (Bathgate) Ltd | Other services | 2018 |
| THE Beige Life Limited | Professional & scientific | 2026 |
| THE Brae Cafe Ltd | Hospitality | 2025 |
| THE Brew Barn (Strathaven) Limited | Other services | 2023 |
| THE Champer Camper Ltd | Wholesale & retail | 2023 |
| THE Coffee Spot Limited | Hospitality | 2006 |
| THE Counselling Network (TCN) Ltd | Health & social care | 2026 |
| THE Crown Inn (Biggar) Limited | Hospitality | 2010 |
| THE Dalserf Haugh Land Partnership | | 1999 |
| THE Deli Carnwath Ltd | Hospitality | 2024 |
| THE Dogchester Ltd | Other services | 2024 |
| THE Elite Outlet Limited | Wholesale & retail | 2023 |
| THE Farm Supply People Ltd | Wholesale & retail | 2013 |
| THE Food Clinic Ltd | Manufacturing | 2026 |
| THE Glazert Project Limited | Hospitality | 2022 |
| THE Glenfruin Development Company Ltd | Construction | 2015 |
| THE Herbal Vets Dispensary Ltd | Professional & scientific | 2025 |
| THE House of Design Ltd | Wholesale & retail | 2026 |
| THE Hungry Housewife’S Eats Ltd | Wholesale & retail | 2019 |
| THE Lanark Print Company Ltd | Manufacturing | 2009 |
| THE Learning Academy (Lanark) Ltd | Wholesale & retail | 2025 |
| THE Lime Rectangle Ltd | Professional & scientific | 2024 |
| THE M.G. Car Company Ltd | Wholesale & retail | 2010 |
| THE Makehouse Limited | Wholesale & retail | 2009 |
| THE Nature Nursery Montessori Ltd | Health & social care | 2020 |
| THE Paper Shop Lanark Limited | Wholesale & retail | 2026 |
| THE Play Barn (Strathaven) Limited | Administrative & support | 2023 |
| THE Playbarn Nolimits Community Interest Company | Health & social care | 2021 |
| THE Playbarn Training Ltd | Education | 2020 |
| THE Pursuit of Constant Improvement Limited | Other services | 2026 |
| THE Rolling Heat Sauna Limited | Other services | 2026 |
| THE Rural Development Trust | Construction | 2004 |
| THE Scene Academy Limited | Professional & scientific | 2025 |
| THE Smarter Heating Company Ltd | Construction | 2022 |
| THE Suite Spot (Glasgow) Ltd | Wholesale & retail | 2024 |
| THE Temptress Tales Limited | Wholesale & retail | 2026 |
| THE WEE Mans Limited | Hospitality | 2005 |
| THE WEE Room Autism Consultancy Ltd | Professional & scientific | 2023 |
| THE West END Grill Limited | Hospitality | 2014 |
| THE Wood Store (Parkhead) Ltd | Wholesale & retail | 2013 |
| Thelastshift Ltd | Hospitality | 2025 |
| Thistle Media Group Limited | Professional & scientific | 2019 |
| Thorough Brand Ltd | Professional & scientific | 2016 |
| Throup Media Ltd | Information & communication | 2026 |
| Tidyhaven Limited | Hospitality | 2024 |
| Tillie Bar & Bistro Limited | Hospitality | 2020 |
| TIM Clark Consultancy Ltd | Professional & scientific | 2026 |
| Tinto Farm Vets Limited | Professional & scientific | 2019 |
| Tinto Property Investments Limited | Real estate | 2025 |
| TLB Holdings Limited | Financial services | 2017 |
| Toledo Industries LP | | 2015 |
| Tomino International Ltd. | Wholesale & retail | 2004 |
| Topflight Group Limited | Manufacturing | 2020 |
| Toploader UK Ltd | Transport & logistics | 2011 |
| Total Control Security Limited | Administrative & support | 2020 |
| Transformation Advisory Services Ltd | Professional & scientific | 2025 |
| Triathlon Workx Limited | Arts & recreation | 2022 |
| Tricel (Lanark) Limited | Manufacturing | 1968 |
| TT Eats Ltd | Hospitality | 2026 |
| Tuned to the Moon Media Limited | Information & communication | 2023 |
| Turris Travel Ltd | Administrative & support | 2013 |
| Tweedie Minitubers Ltd | Extraterritorial | 2026 |
| Twilight Blinds LLP | | 2018 |
| Two Brothers Installations Ltd | Construction | 2019 |
| Two Dugs Ltd | Information & communication | 2025 |
| Uddingston Auto Repair Centre Limited | Wholesale & retail | 2022 |
| Ukus Crypto Alliance Limited | Other services | 2025 |
| Ultimate Business Directory Ltd | Information & communication | 2022 |
| Ultimate Uplifts Ltd | Water & waste | 2020 |
| Unique Limo and Tour Experience Ltd | Transport & logistics | 2025 |
| Unique Roofclad Ltd | Construction | 2018 |
| Unleash the Dogs Ltd | Arts & recreation | 2022 |