| 18 Cleveland Road Ltd | Real estate | 2026 |
| A & S Business Services Limited | Professional & scientific | 2003 |
| AC Kelly Ltd | Mining & quarrying | 2025 |
| Achie Farms | | 1986 |
| Alca Torda Dive Limited | Agriculture & fishing | 2019 |
| ALL Natural ME Ltd | Wholesale & retail | 2012 |
| Ambicare Limited | Health & social care | 2018 |
| Argosy Fishing Limited | Agriculture & fishing | 2011 |
| Auchenhay Ltd | Administrative & support | 2016 |
| Ayrshire Railway Preservation Group | Transport & logistics | 1989 |
| Balmangan Farming Co | | 1988 |
| Balmangan Farming Company | | 1995 |
| Balmangan Farms Limited | Agriculture & fishing | 2007 |
| Balmangan Limited | Agriculture & fishing | 2019 |
| Barncrosh Leisure Company Limited | Hospitality | 1976 |
| Beltie Ltd | Information & communication | 2026 |
| Bishopton Company Limited | Agriculture & fishing | 2019 |
| Bossman Street Food Limited | Hospitality | 2025 |
| C & L Savin Limited | Real estate | 2025 |
| Callan Media Ltd | Information & communication | 2013 |
| Carstramon Holdings Co Ltd | Financial services | 2025 |
| Castle Maclellan Foods Limited | Manufacturing | 1982 |
| Catherinefield Properties Ltd | Real estate | 2010 |
| CK House Group Ltd | Hospitality | 2024 |
| Classic Desserts Topco Limited | Manufacturing | 2012 |
| Coffee in Code OUT Ltd | Information & communication | 2023 |
| COW First Nutrition Limited | Agriculture & fishing | 2010 |
| Cree Valley Herbs Limited | Hospitality | 2019 |
| Culraven Limited | Agriculture & fishing | 2013 |
| Cumstoun Farms | | 1991 |
| Dgwgo Limited | Information & communication | 2014 |
| Dunbar Consulting Limited | Professional & scientific | 2013 |
| Earth AID Group Limited | Wholesale & retail | 2016 |
| Fake Food Ltd | Manufacturing | 2025 |
| Faye Armour Ingleston Farm Ltd | Agriculture & fishing | 2020 |
| G W Davidson Ltd | Construction | 2002 |
| Galloway Clinic Ltd | Other services | 2024 |
| Galloway Eggs Limited | Wholesale & retail | 1978 |
| Galloway Wildlife Conservation Trust | Arts & recreation | 2011 |
| Galtway Farm Limited | Agriculture & fishing | 2020 |
| Gladstone House Properties Ltd | Wholesale & retail | 2023 |
| Goodare & Graham Ltd | Manufacturing | 2024 |
| Hayton Coulthard Transport Limited | Transport & logistics | 1996 |
| High Nunton Solar Limited | Construction | 2020 |
| I. Wilson & SON Limited | Construction | 2005 |
| James Smith Fencing Holdings Limited | Financial services | 2026 |
| James Smith Fencing Limited | Manufacturing | 2014 |
| Kathryn King Designer Jeweller Limited | Manufacturing | 2002 |
| Kempleton Cars Ltd | Wholesale & retail | 2012 |
| Kevin Durham (Builders) Limited | Construction | 2003 |
| Kirkcudbright Book Festival CIC | Arts & recreation | 2025 |
| Kirkcudbright Dark Space Limited | Education | 2021 |
| Kirkcudbright Dental Surgery Limited | Health & social care | 2017 |
| Kirkcudbright Development Silvercraigs Caravan Park Limited | Hospitality | 2017 |
| Kirkcudbright Fringe Festival CIC | Arts & recreation | 2024 |
| Kirkcudbright Scallop Gear Limited | Manufacturing | 1973 |
| Kirkcudbright Summer Festivities | Arts & recreation | 2004 |
| Kirkcudbright Swimming Pool Limited | Other services | 1990 |
| Klaro Partners Limited | Manufacturing | 2012 |
| Loganbridge II Limited | Transport & logistics | 2014 |
| M & J G Dunlop | | 1977 |
| Maclay & Corrie | | 1979 |
| Marc a Henkelmann Limited | Professional & scientific | 2010 |
| Margaret Patterson Ltd | Hospitality | 2014 |
| Masonic Arms Limited | Hospitality | 2013 |
| Mass Timber Solutions UK Ltd | Manufacturing | 2018 |
| Meadowhead Estates Company | Agriculture & fishing | 1962 |
| Millar’S L’Atelier DU VIN Ltd | Hospitality | 2024 |
| Moatwell Leisure Ltd | Hospitality | 2025 |
| Money Spider Properties Ltd | Construction | 2020 |
| Moorburn Industries Limited | Wholesale & retail | 2005 |
| Moove Studios Ltd | Education | 2026 |
| Near and Bar Melrose Ltd | Hospitality | 2025 |
| Octopus Energy Wind Works Limited | Professional & scientific | 2021 |
| ON the Deck Ltd | Hospitality | 2024 |
| Organii Limited | Wholesale & retail | 2012 |
| Orroland Holdings Limited | Real estate | 2012 |
| Phoenix Love Limited | Hospitality | 2025 |
| Portobello Hospitality Limited | Hospitality | 2025 |
| Pravera Ltd | Wholesale & retail | 2001 |
| Rancher Livestock Ltd | Extraterritorial | 2018 |
| Reachwill Ltd. | Information & communication | 2007 |
| Rockbright Limited | Wholesale & retail | 2017 |
| Rodger Property Rentals Ltd | Real estate | 2022 |
| Rogerson Motors Limited | Professional & scientific | 1974 |
| Saints Solar Ltd | Other services | 2026 |
| Saviour Associates Ltd | Professional & scientific | 2014 |
| Sithole Cabs Ltd | Transport & logistics | 2024 |
| Solway Animal Health Consultancy Ltd | Professional & scientific | 2020 |
| Solway Feeders Limited | Wholesale & retail | 1994 |
| Solway Global Limited | Financial services | 2010 |
| ST Cuthbert Wanderers FC Ltd | Arts & recreation | 2025 |
| Sun Commercial Ltd | Real estate | 2024 |
| Swim School SC Limited | Education | 2022 |
| Tartan Ribbon Ltd | Wholesale & retail | 2021 |
| Taste the Sea Limited | Manufacturing | 1998 |
| Tasty Tides Ltd | Hospitality | 2024 |
| Tempeh Today Limited | Wholesale & retail | 2016 |
| THE Cocoa Bean Company Limited | Manufacturing | 2005 |
| THE RE: Sale Park Ltd | Wholesale & retail | 2022 |