| Rosemount Nursery School Limited | Education | 2009 |
| Ross & Mackay Whisky Co Ltd | Wholesale & retail | 2020 |
| Russell and Telfer Limited | Construction | 1966 |
| Russell Combe Haulage Limited | Transport & logistics | 2014 |
| S. Koronka (Manufacturing) Limited | Manufacturing | 1974 |
| Salen Pier Limited | Agriculture & fishing | 2012 |
| Samurai-San Ltd | Hospitality | 2020 |
| Sandy Baird Limited | Manufacturing | 2008 |
| Sarkis (Scotland) Limited | Professional & scientific | 2021 |
| Scaramoosh Limited | Construction | 2020 |
| Scavaig Ltd | Professional & scientific | 2024 |
| Science Story Ltd | Professional & scientific | 2015 |
| Scientia Medical Writing Ltd | Professional & scientific | 2023 |
| Scotia Tarmac Ltd | Construction | 2019 |
| Scottish Anglers National Association Limited | Other services | 2006 |
| Scottish Gliding Union - Walking on Air | Arts & recreation | 1996 |
| Scottish Lavender Limited | Arts & recreation | 2024 |
| Scottish Lavender Oils Limited | Manufacturing | 2019 |
| SE Watson Consulting Limited | Manufacturing | 2006 |
| Senako Ltd | Construction | 2019 |
| Seventy SIX Digital Limited | Professional & scientific | 2026 |
| Shand Design Limited | Professional & scientific | 2014 |
| Shedring Hobbies L.P. | | 2016 |
| Sigma Communications and Marketing Limited | Professional & scientific | 2003 |
| Simplicity Funeral Services (Scotland) Limited | Professional & scientific | 2001 |
| Sinclair Legal Limited | Professional & scientific | 2010 |
| Sinclair Services Consultancy Limited | Real estate | 2025 |
| Single Malt Strategy Limited | Information & communication | 2025 |
| Sirius TV Productions Ltd | Information & communication | 2010 |
| SKM Aesthetics Ltd | Health & social care | 2016 |
| Small Batch Bottlers Scotland Limited | Wholesale & retail | 2014 |
| Smartworks Accountants Limited | Professional & scientific | 2015 |
| Solar Wind Limited | Professional & scientific | 2016 |
| SOS Moves Ltd | Transport & logistics | 2019 |
| Speight Digital Ltd | Information & communication | 2018 |
| Spektrum Limited | Extraterritorial | 2018 |
| Square and Bridge Limited | Real estate | 2019 |
| ST Transport Scotland Ltd | Transport & logistics | 2025 |
| Stapleton Rental Solutions Ltd | Real estate | 2025 |
| Station Storage Limited | Transport & logistics | 2017 |
| Station View Consultancy Limited | Information & communication | 2005 |
| Stewart Funeral Directors Limited | Professional & scientific | 1990 |
| Still Consulting (Scotland) Limited | Professional & scientific | 2024 |
| Stirling Blue Events Limited | Administrative & support | 2014 |
| STM Recovery Ltd | Manufacturing | 2025 |
| Stoddart Construction Services Limited | Professional & scientific | 2016 |
| Stovestuff Ltd | Construction | 2015 |
| Stuart Hamilton Limited | Arts & recreation | 2018 |
| STZ Brickwork Ltd | Construction | 2015 |
| Sugarin UK Limited | Wholesale & retail | 2020 |
| Sunono Publishing Ltd | Information & communication | 2018 |
| Sunrise One Limited | Hospitality | 2024 |
| Tait and Leggat Limited | Health & social care | 2008 |
| TAN ME Salons Ltd | Other services | 2025 |
| Tartan Kipper Ltd | Wholesale & retail | 2023 |
| Tayside Recovery Service Ltd | Wholesale & retail | 2022 |
| Tenor Consulting Limited | Professional & scientific | 2010 |
| TF Consultancy Services Ltd | Administrative & support | 2026 |
| Tghc (Dunning) Limited | Construction | 2025 |
| Tghc (Scotland) Limited | Construction | 2010 |
| Tghc (Wolfhill) Limited | Construction | 2025 |
| Tghc Holdings Limited | Professional & scientific | 2025 |
| THE Denise Difference Ltd | Administrative & support | 2025 |
| THE Distillers' Art Limited | Manufacturing | 2019 |
| THE Glenfinnan Consultancy Limited | Professional & scientific | 2003 |
| THE Good House Company Scotland Limited | Construction | 2005 |
| THE Green Hotel Limited | Hospitality | 2016 |
| THE Ideas DR Ltd | Professional & scientific | 2025 |
| THE Kilted Christmas Tree Company Limited | Agriculture & fishing | 2006 |
| THE Kind Pantry Limited | Wholesale & retail | 2023 |
| THE Kinross Brewery Limited | Manufacturing | 2016 |
| THE Kinross Community Council Newsletter Limited | Professional & scientific | 2009 |
| THE Kinross-Shire Partnership Limited | Professional & scientific | 1998 |
| THE Lendrick Hill Partnership | | 1991 |
| THE Real Fresh Chip Company Limited | Extraterritorial | 2020 |
| THE Service Shop Limited | Manufacturing | 2014 |
| THE Tack Room Milnathort Ltd | Wholesale & retail | 2018 |
| THE Thai Bar & Restaurant Ltd | Hospitality | 2018 |
| Thistle Turnings Ltd | Wholesale & retail | 2018 |
| Thomson Homes Limited | Construction | 2003 |
| Thomson Homes Scotland Limited | Construction | 2015 |
| Thomson Project Management (Scotland) Ltd | Construction | 2019 |
| Threeeights Consultants Limited | Professional & scientific | 2025 |
| Todd & Duncan Limited | Manufacturing | 2009 |
| Together Systems Limited | Construction | 2020 |
| Topline Scotland Ltd | Construction | 2016 |
| Trance Code Ltd. | Information & communication | 2001 |
| Tranent Grove Ltd | Construction | 2023 |
| Trax Plant Hire Ltd | Administrative & support | 2018 |
| Trilogy Services (Scotland) Ltd. | Construction | 2010 |
| Trmb Limited | Arts & recreation | 2016 |
| Turnbrock Limited | Financial services | 2016 |
| TVC Limited | Other services | 2020 |
| TWS D1 Limited | Real estate | 2009 |
| TWS D28 Limited | Real estate | 2009 |
| TWS D35 Limited | Real estate | 2009 |
| Udny Arms Hotel & Trellis Ltd | Hospitality | 2025 |
| Ulla Group Ltd | Real estate | 2025 |
| Unconventional Drilling Limited | Professional & scientific | 2025 |
| Unorthodox Roasters Limited | Manufacturing | 2016 |