| Sally Collins Limited | Professional & scientific | 2022 |
| Sam's Kitchen (2024) Limited | Hospitality | 2024 |
| Samrose (Hull) Limited | Construction | 2023 |
| Sandal Distribution Limited | Professional & scientific | 2021 |
| Sassy Strawberry Gifts Limited | Wholesale & retail | 2017 |
| Saville Hair Limited | Other services | 2010 |
| Scents Sisters Ltd | Manufacturing | 2025 |
| Seddon Travel Limited | Transport & logistics | 2020 |
| Shaw Systems Limited | Information & communication | 2008 |
| Ships and Pigs Limited | Professional & scientific | 2014 |
| Shipton Properties Limited | Construction | 1991 |
| Shopdisk Limited | Hospitality | 2005 |
| Signature Property Finance Specialists Limited | Financial services | 2025 |
| Simig Ltd | Hospitality | 2010 |
| Sitatiny Ltd | Real estate | 2019 |
| SJP24 Limited | Real estate | 1998 |
| Space 4 Hire Limited | Extraterritorial | 2004 |
| SPI Interiors Limited | Construction | 2019 |
| Spin-It Records Limited | Wholesale & retail | 2022 |
| Stamford Mechanical Services Ltd | Construction | 2017 |
| Stansfields Fragrance Oils Limited | Professional & scientific | 2018 |
| Stansfields Properties Limited | Real estate | 2022 |
| Stonebow House (York) Limited | Professional & scientific | 2020 |
| Stratton Oakland Group Limited | Financial services | 2019 |
| Streetwise Business Accountancy Limited | Professional & scientific | 2010 |
| Sunseeker Holiday Homes Limited | Manufacturing | 2019 |
| Swift PAT Testing Ltd | Construction | 2024 |
| Sylwia Property Limited | Real estate | 2022 |
| T E 360 Limited | Construction | 2016 |
| Team D J Ltd | Real estate | 2017 |
| THE Anchor (Spalding) Limited | Hospitality | 2013 |
| THE Dolls House Hessle Limited | Other services | 2019 |
| THE Hull City Official Supporters Club Limited | Wholesale & retail | 2004 |
| THE International Property Law Centre LLP | | 2008 |
| THE Kiterunners Ltd | Financial services | 2020 |
| THE Old Smokehouse Limited | Real estate | 2019 |
| THE Print Works (Hull) Limited | Manufacturing | 1997 |
| Thermica Limited | Manufacturing | 1986 |
| Thomas Mawer (EBT) Limited | Financial services | 2024 |
| Thomas Mawer Group Ltd | Financial services | 2016 |
| Thomas Mawer Limited | Wholesale & retail | 1977 |
| Thomas Mawer Trustee Limited | Administrative & support | 2020 |
| Thousandeyes Security Services Ltd | Administrative & support | 2025 |
| Timarjo Properties Limited | Agriculture & fishing | 2012 |
| Tiny Rock Studios Ltd | Other services | 2025 |
| TJP Projects Limited | Real estate | 2016 |
| TMX Clothing Supplies Limited | Professional & scientific | 2016 |
| Toff's Haulage Ltd | Transport & logistics | 2019 |
| Total Flooring (Hull) Limited | Construction | 2010 |
| Total Service Hull Limited | Wholesale & retail | 2014 |
| Towse Motors Limited | Wholesale & retail | 2014 |
| Tree First Ltd. | Professional & scientific | 2018 |
| TWD Services Limited | Extraterritorial | 1997 |
| Uhpas Trustees Limited | Financial services | 2013 |
| Uktracklighting Limited | Professional & scientific | 2019 |
| Uplight Technical Solutions Ltd | Arts & recreation | 2022 |
| Uppercutz Paragon Street Hull Limited | Other services | 2022 |
| Upvc Holdings Ltd | Financial services | 2023 |
| Upvc Options Limited | Manufacturing | 2021 |
| VIP Events Hull Ltd | Hospitality | 2023 |
| Vulcan Design, Print & Signs Limited | Manufacturing | 2020 |
| Vwcc Limited | Wholesale & retail | 2014 |
| W Stafford Building Services Limited | Construction | 2017 |
| Walsh Property Management Limited | Professional & scientific | 2019 |
| Wexel Gaming Limited | Arts & recreation | 2011 |
| Whitakers Estate Agents Limited | Real estate | 2009 |
| Willerby Education Limited | Administrative & support | 2021 |
| Willow Tree Therapy Services Limited | Health & social care | 2022 |
| Wilson & Wilson (Hull) Limited | Other services | 2023 |
| Wilson & Wilson Group Ltd | Financial services | 2019 |
| Wireless Community Limited | Administrative & support | 2023 |
| Wisper Properties Limited | Real estate | 2020 |
| Workgroup Events Ltd | Administrative & support | 2021 |
| York Cardiology Limited | Health & social care | 2018 |
| YOU SAY Ltd | Administrative & support | 2014 |
| Yupelet 2 Ltd | Real estate | 2008 |
| ZK Global Ltd | Wholesale & retail | 2025 |