| 360 Growth Ltd | Professional & scientific | 2023 |
| A J Autolecs Limited | Manufacturing | 2003 |
| Abbey Screen and Digital Print Ltd | Manufacturing | 1999 |
| Abbeyton Group Ltd | Wholesale & retail | 2015 |
| Access UK Scaffolding Ltd | Construction | 2020 |
| Accm Electrical Services Ltd | Construction | 2025 |
| Adam Tait Haulage Limited | Transport & logistics | 2022 |
| Adhairalba Ltd | Transport & logistics | 2026 |
| Advanced Surfaces Scotland Ltd | Construction | 2020 |
| Agri Distribution (Scotland) Limited | Wholesale & retail | 2019 |
| Ailsa Business Solutions Ltd | Professional & scientific | 2020 |
| Ailsa Group Ltd | Financial services | 2025 |
| Ailsa Reliability Solutions Ltd | Professional & scientific | 2020 |
| Airshire Ltd | Wholesale & retail | 2020 |
| Alico Building Supplies Ltd | Wholesale & retail | 2023 |
| Alkan Investments Ltd | Real estate | 2025 |
| ALL About the Magic CIC | Hospitality | 2019 |
| ALL Seasons Kitchen Ltd | Hospitality | 2025 |
| Alpine Retail Ltd | Wholesale & retail | 2025 |
| Anteriors (Scotland) Limited | Manufacturing | 2013 |
| AP Civils Scotland Ltd | Administrative & support | 2021 |
| APW Site Services Limited | Professional & scientific | 2020 |
| Ardoch Property Investments Limited | Real estate | 2004 |
| Arenfield Limited | Real estate | 2012 |
| Aston Capital Limited | Real estate | 2018 |
| Atlas Minibus Ltd | Transport & logistics | 2013 |
| ATS Roofing (Ayrshire) Ltd | Construction | 2026 |
| Auto Inspection and Access Limited | Professional & scientific | 2009 |
| Autoprotekt Limited | Manufacturing | 2017 |
| AYE Property Ltd | Real estate | 2024 |
| Ayrshire Access Ltd | Construction | 2018 |
| Ayrshire Fence & Garden Ltd | Administrative & support | 2021 |
| Ayrshire Group Ltd | Real estate | 2015 |
| Ayrshire Rigging/Erecting Ltd | Manufacturing | 2026 |
| Azeez Barbers Ltd | Other services | 2023 |
| Ballantine Psychology Ltd | Education | 2025 |
| BBQ Inn Limited | Hospitality | 2012 |
| Beechgrove Stores Ltd | Wholesale & retail | 2013 |
| Bennetts Kilwinning Limited | Other services | 2017 |
| Benslie Training Limited | Education | 2009 |
| Beth Brownings Bakery Limited | Extraterritorial | 2018 |
| Betteru Fitness Limited | Education | 2020 |
| BJH Solutions Ltd | Wholesale & retail | 2024 |
| Black Moon Bakehouse Ltd | Hospitality | 2025 |
| Blackland Property Ltd | Real estate | 2019 |
| Blackland Retail Ltd | Wholesale & retail | 2019 |
| Bonnie Things CIC | Manufacturing | 2026 |
| Bridgend Accident Repair Centre Limited | Wholesale & retail | 1986 |
| Bridgend Estates Limited | Professional & scientific | 1999 |
| Bridgend Garage Limited | Wholesale & retail | 1986 |
| Bridgend Holdings Limited | Financial services | 1997 |
| Bridgend Motor Group Ltd | Extraterritorial | 2015 |
| Bridgend Services Limited | Extraterritorial | 1988 |
| Brja Property Ltd | Real estate | 2023 |
| Buckreddan Care Centre Limited | Health & social care | 2004 |
| Bull-Works Gates and Railings Ltd | Manufacturing | 2021 |
| Burleigh Aviation Ltd | Professional & scientific | 2013 |
| Burns Macdonald Ltd | Health & social care | 2022 |
| Burrowland Dairy Limited | Agriculture & fishing | 2025 |
| C LEE Engineering Ltd | Manufacturing | 2026 |
| Caledonian Fire Safety Solutions Ltd | Public administration | 2017 |
| Cassino Foods Ltd | Hospitality | 2018 |
| Chapter Holistics Ltd | Other services | 2024 |
| Ciroandfrance Ltd | Hospitality | 2025 |
| Clow Engineering Services Limited | Professional & scientific | 2017 |
| CM Fencing and Landscaping (Scotland) Ltd | Administrative & support | 2024 |
| Coast Bathrooms Limited | Extraterritorial | 2023 |
| Coast Plumbing Supplies Limited | Wholesale & retail | 2020 |
| Coco Maya Ltd | Wholesale & retail | 2024 |
| Collinton Civils (Scotland) Limited | Construction | 2005 |
| Collinton Ltd | Extraterritorial | 2012 |
| Colpipe Services Ltd | Manufacturing | 2026 |
| County Down Holdings Ltd | Real estate | 2020 |
| Couzin Electrical Ltd | Construction | 2020 |
| COX Rail Ltd | Construction | 2016 |
| D & R Fitness Ltd | Arts & recreation | 2025 |
| D & R Properties Ayrshire Ltd | Real estate | 2021 |
| D and S 2024 Services Limited | Other services | 2024 |
| D MAC Pipework Ltd | Professional & scientific | 2026 |
| D. Burns & SON Limited | Construction | 2006 |
| DC Welding Solutions Ltd | Construction | 2018 |
| DGM Group Ltd | Health & social care | 2023 |
| Dimwicks Ltd | Wholesale & retail | 2022 |
| Direct Property Agents Ltd | Real estate | 2024 |
| DM ARB Limited | Administrative & support | 2019 |
| DML Systems Limited | Information & communication | 2003 |
| Dram Letting Ltd | Households | 2025 |
| DRM Maximus Ltd | Construction | 2025 |
| DS Contracts Scotland Limited | Construction | 2017 |
| Durnan Consulting Ltd. | Information & communication | 2013 |
| DWT Electrical Services Limited | Construction | 2017 |
| Ecocel Energy (Storage) Ltd | Construction | 2022 |
| Ecocel Services Ltd | Manufacturing | 2015 |
| Eglinton Builders Ltd | Construction | 2024 |
| Elccino Cappuccino Ltd | Hospitality | 2026 |
| Elements Heating and Energy Ltd | Construction | 2026 |
| ELEV8 Estates Ltd | Administrative & support | 2023 |
| Elite Chores Ltd | Transport & logistics | 2025 |
| Elite Paving Limited | Administrative & support | 2015 |
| Embroidery Solutions Scotland Limited | Wholesale & retail | 2019 |