| Ayrshire Estates Limited | Real estate | 1994 |
| Ayrshire EV Ltd | Construction | 2025 |
| Ayrshire Film Company Community Interest Company | Information & communication | 2014 |
| Ayrshire Fire & Security Limited | Administrative & support | 2017 |
| Ayrshire FIX and Fit Ltd | Construction | 2026 |
| Ayrshire Food Hub Ltd | Wholesale & retail | 2017 |
| Ayrshire Golf Collective Limited | Administrative & support | 2026 |
| Ayrshire Heating Solutions Ltd | Professional & scientific | 2012 |
| Ayrshire Landscapes Ltd | Administrative & support | 2021 |
| Ayrshire Power Cleaning Limited | Administrative & support | 2020 |
| Ayrshire Property Group Ltd | Real estate | 2020 |
| Ayrshire Property Services Ltd | Construction | 2018 |
| Ayrshire Renovations Ltd | Construction | 2021 |
| Ayrshire Rope Access Ltd | Construction | 2025 |
| Ayrshire Signs & Graphics Limited | Professional & scientific | 2011 |
| Ayrshire Simple Funerals Ltd | Other services | 2017 |
| Ayrshire Systems Ltd | Wholesale & retail | 2016 |
| Ayrshire Tattoo Convention Ltd | Hospitality | 2025 |
| Ayrshire Timber Works Ltd | Manufacturing | 2025 |
| Ayrshire Training Services Ltd | Education | 2020 |
| Ayrshire Tree Services Ltd | Agriculture & fishing | 2026 |
| Ayrshire Van Services Ltd | Transport & logistics | 2025 |
| Ayrshire Warriors Ltd | Arts & recreation | 2015 |
| Ayrshire Washroom & Clinical Waste Disposal Services Ltd. | Other services | 2020 |
| Ayrshire Windows & Doors Ltd | Manufacturing | 2024 |
| Ayrshires Finest Roasters Ltd | Manufacturing | 2025 |
| Azzurri Warranties Ltd | Wholesale & retail | 2024 |
| B-Bop Hair & Beauty Studio Ltd | Other services | 2021 |
| B.M. Grab Hire Services Ltd | Water & waste | 2021 |
| B.M.L. Properties Ltd. | Households | 2001 |
| B.P.D. Solutions Ltd | Financial services | 2011 |
| Babyscan.Me Ltd | Health & social care | 2020 |
| Bacchuspace Ltd | Real estate | 2025 |
| Badaninal Ltd | Professional & scientific | 2019 |
| BAF 13 Ltd | Real estate | 2021 |
| BAG & Luggage Co AYR Limited | Extraterritorial | 2014 |
| Bajjar Brother's Limited | Hospitality | 2026 |
| Balanced Books Ayrshire Ltd | Professional & scientific | 2020 |
| Balanced Books Holdings Ltd | Financial services | 2024 |
| Balbairnie C.I.C. | Information & communication | 2023 |
| Baldelectrician Limited | Construction | 2017 |
| Balerno Holdings Ltd | Real estate | 2018 |
| Balgray Holdings Limited | Financial services | 2018 |
| Ballot Road Properties Limited | Real estate | 2022 |
| Balmoral Mill Shop Limited | Wholesale & retail | 1989 |
| Bamsky Foods Ltd | Hospitality | 2025 |
| Banana Brows Ltd | Other services | 2023 |
| Banhmi Vietfood Ltd | Hospitality | 2024 |
| Bankfield View Ltd | Financial services | 2020 |
| Bar Fast Food Ltd | Hospitality | 2019 |
| Barclay GAS Services Ltd | Construction | 2020 |
| Barrett Commercial Management Ltd | Professional & scientific | 2013 |
| Barry Devlin Holdings Limited | Financial services | 2018 |
| Barry Devlin Services Limited | Wholesale & retail | 2009 |
| Bars Glasgow Limited | Hospitality | 2024 |
| BAS Lubricants Limited | Wholesale & retail | 2003 |
| Base TEN Construction Consultants Ltd | Professional & scientific | 2026 |
| Basmara Traders Ltd | Wholesale & retail | 2024 |
| Bathrooms Plus Ltd | Wholesale & retail | 2021 |
| BC Civil Engineering Ltd | Construction | 2023 |
| BCM Catering Limited | Hospitality | 2025 |
| BD Plumbing & Heating GAS Services Ltd | Construction | 2026 |
| BDG Construction Management Ltd | Construction | 2017 |
| BDG Project Management Ltd | Construction | 2025 |
| BDM Properties Limited | Real estate | 2025 |
| BE Beautiful at One Ltd | Other services | 2024 |
| BE Property Experts Ltd | Real estate | 2018 |
| Beansburn Therapies Ltd | Other services | 2025 |
| Bearing Gifts Troon Limited | Wholesale & retail | 2023 |
| Beaton Digital Ltd | Professional & scientific | 2025 |
| Beauty Spaces (Ayrshire) Limited | Other services | 2016 |
| Beechcoast Property Holdings Limited | Real estate | 2006 |
| Beechwood Finance and Governance Limited | Professional & scientific | 2026 |
| Bel&Raf Rendering Ltd | Construction | 2020 |
| Bell Legal Limited | Professional & scientific | 2013 |
| Bellfield After School Care Ltd. | Health & social care | 2003 |
| Belmondo Rendering Ltd | Construction | 2021 |
| Beresford Accountancy Limited | Real estate | 2012 |
| Berkant Solutions Ltd | Wholesale & retail | 2025 |
| Bernard Mcinnes Ltd | Construction | 2019 |
| Bespoke Wells Ltd | Mining & quarrying | 2017 |
| Bestfood Ardrossan Limited | Hospitality | 2022 |
| Beth Brownings Limited | Wholesale & retail | 2016 |
| Beunique AYR Limited | Wholesale & retail | 2025 |
| Beveridge Property Limited | Real estate | 2022 |
| Beyond Mining Group Ltd | Professional & scientific | 2023 |
| Bfparts Ltd | Wholesale & retail | 2025 |
| BG FFF Coaching LLP | | 2025 |
| BG Holdings (Ayrshire) Ltd | Other services | 2025 |
| BG Invest Ltd | Real estate | 2015 |
| BGS Services Limited | Administrative & support | 2022 |
| BIA Functional Fitness 2.0 Ltd | Arts & recreation | 2016 |
| Bibblon Renewables Limited | Extraterritorial | 2014 |
| Bijoux Brows Academy Limited | Other services | 2023 |
| Bikexplore | Hospitality | 2021 |
| BJA Lifting Services Ltd | Construction | 2012 |
| BK Procon Ltd | Professional & scientific | 2025 |
| Black 5 9 PUB Limited | Hospitality | 2024 |
| Blackwood Holdings Limited | Financial services | 2017 |
| Blackwood Plant Hire Ltd | Administrative & support | 2003 |