| AB Works Limited | Construction | 2018 |
| ALL Banter & Booze Ltd | Other services | 2025 |
| Aquafil UK Limited | Manufacturing | 2014 |
| Aspris (Troup House) Limited | Administrative & support | 2005 |
| Aspris Scotland Holdings Limited | Financial services | 2012 |
| Aspris Scotland Limited | Administrative & support | 2010 |
| Attix CIC | Administrative & support | 2017 |
| Ayrshire Tree Surgeons Limited | Agriculture & fishing | 2008 |
| Barnarlo Design Limited | Professional & scientific | 2026 |
| BAS Builders Ltd | Construction | 2026 |
| BLU Bar and Restraunt Ltd | Wholesale & retail | 2024 |
| Blue Line Tactical Solutions Limited | Wholesale & retail | 2019 |
| Blueprint Building Solutions Ltd | Construction | 2024 |
| Blush Pearl Events Ltd | Hospitality | 2021 |
| BMR (Scotland) Ltd. | Wholesale & retail | 2014 |
| Brickwall Builder’S Limited | Construction | 2025 |
| Bridgend Foods Limited | Hospitality | 2023 |
| Brown Properties and Developments Ltd | Construction | 2018 |
| Caledonian Classic Arms Limited | Wholesale & retail | 2013 |
| Catabasis Limited | Professional & scientific | 2019 |
| CJM Tissue Release Ltd | Other services | 2016 |
| Clearview Property Services Ltd | Administrative & support | 2025 |
| Clyde Coast Access Services Limited | Construction | 2010 |
| Co-Ordinate Design Limited | Professional & scientific | 2015 |
| Crawford Young Contractors Ltd | Administrative & support | 2026 |
| CTJ Solutions Ltd | Professional & scientific | 2025 |
| CTS (Cairns Training Services) Ltd | Professional & scientific | 2026 |
| Davjet Ltd | Transport & logistics | 2019 |
| Deep Loch Games Ltd | Information & communication | 2024 |
| Deryck's Analytical Limited | Information & communication | 2025 |
| Double Dutch (Scotland) Ltd | Construction | 2016 |
| Eliza Lang Ltd | Professional & scientific | 2010 |
| ESG Services Ltd | Professional & scientific | 2025 |
| Eyeconic Brows & Lashes Ltd | Other services | 2024 |
| Fife Regatta Limited | Arts & recreation | 2003 |
| Finefit Joinery Ltd | Manufacturing | 2026 |
| Forsyte Ltd | Professional & scientific | 2017 |
| Gainsdale Properties Ltd | Real estate | 2017 |
| Galaxy Cafe Limited | Hospitality | 2015 |
| Galaxy UK Leisure Limited | Arts & recreation | 2015 |
| Garnock Solutions Limited | Professional & scientific | 2018 |
| Garnock Valley Car Company Limited | Wholesale & retail | 2008 |
| Garnock Valley Energy Co Ltd | Financial services | 2023 |
| Girard Industries Europe Limited | Manufacturing | 1986 |
| Grafiká Signs Ltd | Manufacturing | 2024 |
| GRS Fencing Ltd | Construction | 2025 |
| Hallidays Catering Limited | Manufacturing | 2019 |
| Hampson Technical Ltd | Construction | 2025 |
| Harvieston Ltd | Information & communication | 2025 |
| Heather&Hide Ltd | Wholesale & retail | 2026 |
| Hera Homes Scotland Ltd | Real estate | 2021 |
| Hestia Investment Partners Ltd | Real estate | 2023 |
| Holmhead Ltd | Construction | 2024 |
| Holmhead Retailing Ltd | Wholesale & retail | 2022 |
| Hynd Construction Ltd | Construction | 2022 |
| ITS BIN Washed Ltd | Administrative & support | 2025 |
| Jack Access Limited | Construction | 2024 |
| Jasmine H Ltd | Hospitality | 2025 |
| JBS Joiners Ltd | Manufacturing | 2017 |
| JNS Uplawmoor Ltd | Hospitality | 2021 |
| Kennedy Haulage Ltd | Transport & logistics | 2024 |
| Kilbirnie Auld Kirk Future Group Ltd | Arts & recreation | 2026 |
| Kilbirnie Hair Style Ltd | Other services | 2026 |
| KK Leisure Ltd | Hospitality | 2020 |
| KOM Motorhomes Ltd | Administrative & support | 2020 |
| LAJ Protective Coatings Ltd | Construction | 2025 |
| Lindsay Davis Ltd | Mining & quarrying | 2012 |
| Lochwinnoch Community Hub Ltd | Education | 2025 |
| Loyal Chef Ltd | Hospitality | 2024 |
| LTW Domestics Limited | Administrative & support | 2024 |
| Lucky House 1818 Limited | Hospitality | 2025 |
| Lunchbox Kilbirnie Limited | Hospitality | 2024 |
| M&M Ritchie Ltd | Information & communication | 2011 |
| Macrocom (1030) Limited | Professional & scientific | 2013 |
| Marc Scott Limited | Manufacturing | 2018 |
| MCD Logistics Ltd | Transport & logistics | 2026 |
| MM Services 111111 Ltd | Administrative & support | 2023 |
| Moorpark Healthcare Limited | Health & social care | 2021 |
| Moorparkgenealogy Limited | Professional & scientific | 2023 |
| Murray Martin Services Limited | Manufacturing | 1995 |
| Networks Bistro Ltd | Hospitality | 2022 |
| Newhouse Store Ltd | Wholesale & retail | 2024 |
| NEX Generation Pest Control Ltd | Administrative & support | 2026 |
| NMA Homes Ltd | Real estate | 2025 |
| NO 19 Ltd | Other services | 2013 |
| Orr's Motorworks Ltd | Wholesale & retail | 2025 |
| PAK Ayrshire Ltd | Hospitality | 2025 |
| Pewtermill Crafts Ltd | Wholesale & retail | 2014 |
| PV Solar Investments Ltd. | Financial services | 2010 |
| Quinn Memorials Ltd | Other services | 2015 |
| Radio City Association Limited | Energy | 1999 |
| Rani Foods Limited | Hospitality | 2024 |
| Reddys Retails Ltd | Wholesale & retail | 2024 |
| Relax@Rids Ltd | Other services | 2025 |
| Renewable Fitting Company Ltd | Construction | 2024 |
| Repz Products Ltd | Wholesale & retail | 2021 |
| RJT Kerbside Ltd | Construction | 2026 |
| RM HPH Ltd | Wholesale & retail | 2024 |
| Rohrstoff Limited | Wholesale & retail | 2004 |
| Romeo & Juliet Inn Limited | Hospitality | 2013 |