| Emeryl Tutoring Limited | Education | 2025 |
| Emferg Education Ltd | Health & social care | 2015 |
| Empire Coachbuilders Ltd | Manufacturing | 2011 |
| Empire Embroidery Limited | Administrative & support | 2007 |
| Employee Tax Planning and Advice Ltd | Extraterritorial | 2013 |
| Empress Aesthetics Limited | Other services | 2025 |
| Empyrean Therapies Ltd | Health & social care | 2025 |
| Emysther Ltd | Health & social care | 2024 |
| Enache Driving Ltd | Transport & logistics | 2020 |
| Encroacher Ltd | Wholesale & retail | 2018 |
| Endless Delusion Ltd | Wholesale & retail | 2025 |
| Enduro Group Holdings Limited | Financial services | 2025 |
| Enduro Industrial Fasteners Holdings Limited | Financial services | 2025 |
| Enduro Industrial Fasteners Limited | Wholesale & retail | 2015 |
| Energi to Zero Ltd | Professional & scientific | 2023 |
| Energy 21 Limited | Wholesale & retail | 1999 |
| Engineering & Building Design Limited | Professional & scientific | 2015 |
| Engines to Zero Ltd | Professional & scientific | 2023 |
| English Roadsters Limited | Extraterritorial | 2005 |
| Engraved Oak Company Limited | Manufacturing | 2016 |
| Enhanced Home Services Ltd | Households | 2023 |
| Enterprise Weaving Company Limited | Manufacturing | 1996 |
| Entogrow Ltd | Agriculture & fishing | 2023 |
| ENVIRO24 Midlands Limited | Water & waste | 2025 |
| Eonezore Ltd | Wholesale & retail | 2023 |
| EP Architecture Ltd | Construction | 2018 |
| EP Therapy Limited | Other services | 2021 |
| Epadusher Ltd | Wholesale & retail | 2023 |
| Epidermatech Innovations Ltd | Wholesale & retail | 2026 |
| Epreneur Enterprises LLP | | 2019 |
| Eps/Jre Ltd | Real estate | 2024 |
| Equesty Ltd | Education | 2025 |
| Equitrain Limited | Wholesale & retail | 2011 |
| ER Data Services Ltd | Information & communication | 2024 |
| ER Procurement Solutions Ltd | Professional & scientific | 2026 |
| Eraofduplicates Ltd | Administrative & support | 2019 |
| Eraterous Ltd | Wholesale & retail | 2018 |
| Ericsson Electronic Commerce Co., Ltd | Wholesale & retail | 2021 |
| Eridge Green Ltd | Hospitality | 2022 |
| Eridge Ltd | Administrative & support | 2022 |
| Ersmerilin Ltd | Health & social care | 2024 |
| Erzanlef Ltd | Wholesale & retail | 2024 |
| Eshlamp Ltd | Transport & logistics | 2021 |
| Essdee (Holdings) Limited | Professional & scientific | 2014 |
| Essocely Ltd | Transport & logistics | 2019 |
| Estate Country Feeds Limited | Administrative & support | 2014 |
| Eszter's Kitchen Ltd | Wholesale & retail | 2022 |
| Etac Limited | Health & social care | 2000 |
| Ethical Influence Limited | Administrative & support | 2005 |
| Euro Systems UK Ltd | Wholesale & retail | 2009 |
| Eurofast Property Holdings Limited | Financial services | 2025 |
| Eurofit Installations Limited | Construction | 2004 |
| Euromillions Ltd | Professional & scientific | 2022 |
| Eurotrans Spedition Ltd | Transport & logistics | 2024 |
| Euvomner Ltd | Wholesale & retail | 2024 |
| EV Charge Solutions Ltd | Construction | 2025 |
| Evans Prototype Engineering Ltd | Manufacturing | 2022 |
| Evans Tanker Services Limited | Administrative & support | 2007 |
| Evcott Construction Limited | Professional & scientific | 2014 |
| Ever After Day Nursery Limited | Education | 2011 |
| Everholly Ltd | Administrative & support | 2019 |
| EVO Pressure and Temperature Ltd | Professional & scientific | 2023 |
| Evolution Exotics Ltd | Professional & scientific | 2022 |
| Ewaddory Limited | Professional & scientific | 2024 |
| EX Hurley Ltd | Transport & logistics | 2022 |
| Ex-Ceed Innovation Limited | Manufacturing | 2014 |
| Exaltez Ltd | Wholesale & retail | 2019 |
| Exchangemyhome Limited | Information & communication | 2025 |
| Exilco Holdings Ltd | Financial services | 2025 |
| Exilco Limited | Manufacturing | 1982 |
| Extramile Maintenance Limited | Construction | 2016 |
| EZ Cotton Ltd | Manufacturing | 2021 |
| Ezee Electrical Limited | Construction | 2017 |
| EZI Floor Limited | Construction | 2016 |
| Ezrasweet Ltd | Wholesale & retail | 2018 |
| Ezthourfe Ltd | Transport & logistics | 2024 |
| F&D Property Ltd | Real estate | 2020 |
| F.A.R Airconditioning & Refrigeration Ltd | Construction | 2025 |
| F14 Steel Excellency Ltd | Manufacturing | 2026 |
| F1T2 Limited | Construction | 2020 |
| Fable and Willow Limited | Extraterritorial | 2015 |
| Fabvent Holdings Ltd | Manufacturing | 2024 |
| Fairburn Limited | Manufacturing | 1999 |
| Fairsong Ltd | Transport & logistics | 2019 |
| Falēn Ltd | Administrative & support | 2025 |
| Family Giveaways Ltd | Other services | 2022 |
| Famtek Security Solutions Ltd | Administrative & support | 2023 |
| FAR Forest Stores Ltd | Wholesale & retail | 2015 |
| Farangtamba Ltd | Professional & scientific | 2019 |
| Farfield Limited | Hospitality | 2023 |
| Farlow Farm Nursery Limited | Agriculture & fishing | 2020 |
| Farmight Limited | Information & communication | 2025 |
| Farrow Training UK Limited | Transport & logistics | 2010 |
| Farzix Ltd | Wholesale & retail | 2024 |
| Fast R Solutions Limited | Real estate | 2016 |
| FAT 2008 Ltd | Hospitality | 2024 |
| FAT Eddies Smoke House Ltd | Hospitality | 2020 |
| Fayishu Ltd | Transport & logistics | 2019 |
| FB Racing Worcester Limited | Agriculture & fishing | 2016 |
| FCG Management Limited | Administrative & support | 2024 |