| DNS Industrial Ltd | Wholesale & retail | 2018 |
| DNT Property Services Ltd | Construction | 2018 |
| Dog Therapy UK Ltd | Other services | 2024 |
| Donato DEL Vecchio Ltd | Professional & scientific | 2025 |
| Dongguan Jiali Industrial Co., Ltd. | Administrative & support | 2021 |
| Dongguan SYK Co Ltd | Administrative & support | 2022 |
| Dongguan XIN Meijia New Material Limited | Administrative & support | 2023 |
| Dongguan Xuancai Clothing Co., Ltd | Administrative & support | 2023 |
| Dongguan Yonghe Trading Co., Ltd | Administrative & support | 2022 |
| Dongtai City Senfu Metal Products Co., Ltd | Administrative & support | 2021 |
| Donuts Computer Technology Ltd | Administrative & support | 2020 |
| Dora Accessories Co., Limited | Extraterritorial | 2018 |
| Double and Thrice Ltd | Information & communication | 2023 |
| Dowell International Trading Co., Ltd | Extraterritorial | 2018 |
| Downe Stone LLP | | 2013 |
| DR Charlotte Oliver Limited | Health & social care | 2024 |
| DR Paul Foster Ltd | Health & social care | 2014 |
| DR Zaid Jasim Medical Services Ltd | Health & social care | 2025 |
| DR. Maria Zahid Limited | Health & social care | 2022 |
| DS Production Services Ltd | Administrative & support | 2025 |
| DSW Automotive Ltd | Professional & scientific | 2016 |
| Dtap Studios Ltd | Professional & scientific | 2026 |
| DTE Solutions Limited | Professional & scientific | 2001 |
| DTH Consultants Limited | Administrative & support | 2018 |
| Dtskill AI Limited | Information & communication | 2026 |
| Durabella Floor Systems Limited | Professional & scientific | 2011 |
| Dusthouse Limited | Information & communication | 2010 |
| Dutton Glass & Mirrors Limited | Wholesale & retail | 1994 |
| DV Carpentry & Building Services Ltd | Construction | 2025 |
| DX Commander Antennas Ltd | Professional & scientific | 2022 |
| Dynacor Ltd | Construction | 2008 |
| Dynamic MRF Group Limited | Information & communication | 2019 |
| Dynasty Studios Ltd | Manufacturing | 2022 |
| E & D Cuningham Property Ltd | Real estate | 2021 |
| E-Warehousing Limited | Administrative & support | 2008 |
| Eagle Trading Limited | Administrative & support | 2014 |
| Earnsford Advisors Limited | Extraterritorial | 2025 |
| East Chase Distilleries Ltd | Manufacturing | 2020 |
| Eastern Star Marine Co., Ltd | Administrative & support | 2019 |
| Eastern Suburbs Ltd | Construction | 2009 |
| Easynote Stationery Co Limited | Wholesale & retail | 2009 |
| Easyway International Group Co., Ltd. | Administrative & support | 2025 |
| Eaves & Gable Ltd | Real estate | 2024 |
| Ebourne Close (56-63) Management Company Limited | Households | 2005 |
| Echo Investments Limited | | 1990 |
| Eckom Auto Parts Limited | Extraterritorial | 2010 |
| Eclat Productions Limited | Professional & scientific | 2022 |
| Eco Windows Ltd | Construction | 2022 |
| Eden&Envy Training Academy Ltd | Education | 2023 |
| Eden's Legends Limited | Manufacturing | 2017 |
| Edge Tackle Limited | Wholesale & retail | 2021 |
| Ediguide Limited | Education | 2025 |
| Edmonds Jewellers Limited | Wholesale & retail | 2017 |
| Educational Musicals Limited | Information & communication | 2001 |
| Edward Paginton Associates Ltd. | Information & communication | 2017 |
| Edwin Knight Therapy Limited | Health & social care | 2016 |
| Efan Daily Chemical Co., Limited | Administrative & support | 2011 |
| EG Properties Ltd | Real estate | 1999 |
| EH Woodcote Limited | Construction | 2009 |
| EJH Medical Consulting Ltd | Health & social care | 2025 |
| EJS Communications Limited | Information & communication | 2026 |
| ELBCO2008 Limited | Administrative & support | 2008 |
| Electrical Options Limited | Information & communication | 2010 |
| Electrical Waste Limited | Water & waste | 2008 |
| Elfega Properties Ltd | Real estate | 2022 |
| Elite Analytix Ltd | Information & communication | 2018 |
| Elite Heat Heating and Plumbing Ltd | Construction | 2020 |
| Elizabeth Davenport Limited | Real estate | 2012 |
| Ellie Louise Beauty Ltd | Other services | 2021 |
| Elliot Cleaning Bars Limited | Hospitality | 2022 |
| ELM Farm SPV Limited | Construction | 2019 |
| Elsa Media Technology Co., Ltd | Information & communication | 2024 |
| Elta Estates Limited | Real estate | 2014 |
| Elthamwasp Properties Limited | Real estate | 2020 |
| Elthamwasp Services Limited | Extraterritorial | 2021 |
| Emma Greenwood Consulting Limited | Administrative & support | 2021 |
| Emma Mortgages Ltd | Financial services | 2023 |
| Emmagrange Limited | Construction | 1978 |
| Emmerson Holdings Limited | Financial services | 2015 |
| Emperor International Art Ltd | Extraterritorial | 2019 |
| Empower and Rise CIC | Health & social care | 2025 |
| Empower Health and Wellbeing Limited | Health & social care | 2024 |
| Emscote Management Limited | Real estate | 2014 |
| Enabling Spaces CIC | Health & social care | 2017 |
| Endurance GB | Arts & recreation | 2001 |
| Energy and Utilities Alliance (EUA) | Administrative & support | 2016 |
| Energy Planning Limited | Extraterritorial | 2021 |
| Energy Services and Technology Association Limited | Other services | 1982 |
| Energywise (Midlands) Ltd | Construction | 2024 |
| Engaged Property Limited | Real estate | 2017 |
| Enhanced Mobility Limited | Wholesale & retail | 2013 |
| Enjoysearch Limited | Information & communication | 2025 |
| Ennate Limited | Manufacturing | 2006 |
| Enodo Project Services Limited | Professional & scientific | 2014 |
| Enra Holdings LLP | | 2025 |
| Enra Jade LLP | | 2025 |
| Enra Jade Propco LLP | | 2025 |
| Enra Partners Limited | Construction | 2024 |
| Ensco 812 Limited | Financial services | 2010 |
| Ensco Acquisitions Limited | Financial services | 2020 |