| Lochside Farm | | 1989 |
| Lochtower Jewel Ltd | Financial services | 2020 |
| Lowland Lettings Ltd | Extraterritorial | 2010 |
| Loyd & Townsend-Rose Limited | Administrative & support | 1998 |
| LR & RC Holdings Ltd | Real estate | 2023 |
| LRM Forestry Limited | Agriculture & fishing | 2020 |
| M Johnston Properties Limited | Real estate | 2026 |
| M-Pulsive Dance School Ltd | Education | 2025 |
| MAC + JON Limited | Financial services | 2025 |
| Macgordon Ltd | Financial services | 2023 |
| Mactimber Ltd | Agriculture & fishing | 2024 |
| Magnums & More Ltd | Wholesale & retail | 2013 |
| Marciano's Delights Limited | Wholesale & retail | 2020 |
| Maritime Quality Management Ltd | Professional & scientific | 2022 |
| Martins Publishing Services Limited | Information & communication | 2012 |
| Masterz of Glass Limited | Other services | 2015 |
| Matts Kelso Ltd | Other services | 2026 |
| Maxwell Motors Limited | Wholesale & retail | 2003 |
| Mccrock Holdings Ltd | Financial services | 2025 |
| Mcdonald Kane Limited | Administrative & support | 2014 |
| Mclachlan & Wight Builders Ltd | Construction | 2023 |
| Mclachlan & Wight Ltd | Manufacturing | 2019 |
| MD Dancewear Ltd | Wholesale & retail | 2020 |
| Melville Nursery Limited | Real estate | 2017 |
| Merlin Salus PAM Ltd | Professional & scientific | 2015 |
| Merlin Simple Payment Solutions Ltd | Professional & scientific | 2021 |
| Merlin VET Export Ltd | Professional & scientific | 2023 |
| Merlin VET Ltd | Wholesale & retail | 2013 |
| Merlinvet-Cel Ltd | Professional & scientific | 1983 |
| Merse FP | | 2002 |
| Messrs R Flannigan Limited | Construction | 2014 |
| Messrs Robson Limited | Agriculture & fishing | 2009 |
| Michael Ramage Forestry Ltd | Agriculture & fishing | 2017 |
| Mind Screen Limited | Education | 1995 |
| Minto Property Portfolio Ltd | Real estate | 2024 |
| Misco Farming | | 1999 |
| Mitchell Harrison Residential Limited | Construction | 2006 |
| Mmis 2 Store Ltd | Wholesale & retail | 2018 |
| MMIS3 Ltd | Wholesale & retail | 2020 |
| Mo's China Red Limited | Hospitality | 2019 |
| Monocle Content Creation Ltd | Professional & scientific | 2025 |
| Montali Energy Services Ltd | Construction | 2012 |
| Morrison Financial Services Ltd. | Administrative & support | 2005 |
| Mosstower Limited | Agriculture & fishing | 2012 |
| Murray Electrical Ltd. | Construction | 2006 |
| Murray Rural Services Ltd | Professional & scientific | 2024 |
| Muscle Clean Ltd | Administrative & support | 2015 |
| My Handmade Kitchen Limited | Wholesale & retail | 2018 |
| Naked Sourdough Limited | Manufacturing | 2021 |
| Nanotech Dental UK Ltd | Wholesale & retail | 2021 |
| Nenthorn Community Company | Other services | 2018 |
| Neon Training Limited | Education | 2024 |
| New Smailholm Construction Limited | Construction | 2026 |
| New Smailholm Holdings Limited | Financial services | 2026 |
| Nicolson Contracting Ltd | Agriculture & fishing | 2024 |
| Niddrie Development Company Limited | Professional & scientific | 1997 |
| Nifty Campers Ltd | Wholesale & retail | 2024 |
| Nightingales Home Help Services Ltd | Other services | 2013 |
| Northcom Support Limited | Information & communication | 1996 |
| Northern Silver Limited | Extraterritorial | 2024 |
| NS3C Ltd | Professional & scientific | 2022 |
| Olivers Transport Limited | Transport & logistics | 1989 |
| OR Investments Limited | Other services | 2019 |
| Outcast Distribution Ltd | Wholesale & retail | 2022 |
| Oxnam Holdings Limited | Other services | 2023 |
| P J Plant Hire & Groundworks Limited | Construction | 2007 |
| PD Pegasus Ltd | Construction | 2018 |
| Peach WAX Ltd | Education | 2025 |
| Peal Holdings Ltd | Real estate | 2026 |
| Performance Feeds Limited | Wholesale & retail | 2022 |
| Pest Force Scottish Borders Limited | Agriculture & fishing | 2025 |
| Peter Tait Hygiene Supplies Limited | Wholesale & retail | 2014 |
| Phil Stewart Limited | Construction | 2002 |
| Pipeserve Limited | Construction | 2017 |
| PJ Ventures Ltd | Real estate | 2022 |
| Playfair Farming Limited | Agriculture & fishing | 2025 |
| Plexus Corp. Services (UK) Limited | Administrative & support | 2014 |
| Prism Psychology Ltd | Health & social care | 2021 |
| Promotif Limited | Professional & scientific | 2003 |
| Pyramid Travel Products Holdings Limited | Financial services | 2024 |
| Pyramid Travel Products Limited | Manufacturing | 2023 |
| Quantum Life Coaching Ltd | Health & social care | 2018 |
| Queen's House (Kelso) Limited | Health & social care | 2016 |
| R & D Systems Ltd | Construction | 2025 |
| R Hutton Energy Ltd | Construction | 2020 |
| R P & a Freeland-Cook | | 1984 |
| R Plus Racing Ltd | Arts & recreation | 2024 |
| R S Publications Limited | Information & communication | 2011 |
| R. I. Wilson & Grandsons Limited | Transport & logistics | 2016 |
| R.P.B. Pressen Hill Ltd. | Agriculture & fishing | 1997 |
| Radio Borthwick Ltd | Information & communication | 2023 |
| Raffles Brown Ltd | Construction | 2025 |
| Ragiarc Limited | Financial services | 2024 |
| Rallysport Connections Limited | Wholesale & retail | 2019 |
| Range Studio Limited | Professional & scientific | 2021 |
| Real-E-Racing Limited | Information & communication | 2025 |
| Redpath Tyres Holdings Limited | Financial services | 2024 |
| Redpath Tyres Limited | Wholesale & retail | 1979 |
| Rennie Livestock Limited | Agriculture & fishing | 2020 |
| Rennie Welch (Trustees) Limited | Professional & scientific | 2017 |