| 2BE Development Consultancy Limited | Professional & scientific | 2009 |
| 48 Property Ltd | Real estate | 2025 |
| A Mcelrath Limited | Health & social care | 2018 |
| A P Innes Ltd | Agriculture & fishing | 2011 |
| A. Hume (Outfitters) Limited | Wholesale & retail | 2007 |
| A2Z Property Sourcing Ltd | Real estate | 2022 |
| Abbey Fitness Centre Limited | Arts & recreation | 2008 |
| Abbey Tool and Gauge Limited | Professional & scientific | 2015 |
| Aberdeen Tutors Limited | Education | 2024 |
| Agrisile Ltd | Wholesale & retail | 2020 |
| AHA Property Management Limited | Real estate | 2021 |
| AHO Holdings Limited | Financial services | 2020 |
| Aitken Turnbull Architects Limited | Professional & scientific | 2003 |
| Ajdj Properties Ltd | Administrative & support | 2020 |
| AJM Financial Planning Limited | Financial services | 2019 |
| Alarms for Farms Limited | Administrative & support | 2011 |
| Aldent Ltd | Health & social care | 2025 |
| Aldo Fish Bar Limited | Hospitality | 2019 |
| Alexander Wait & Sons Limited | Transport & logistics | 2016 |
| Alexdule Ltd | Health & social care | 2025 |
| Alison Mcdougal Style Ltd | Other services | 2017 |
| Allan Campbell GAS Services Ltd | Manufacturing | 2021 |
| Amira Fast Food Ltd | Hospitality | 2017 |
| AN Advisory Ltd | Professional & scientific | 2022 |
| AND WE DO This Ltd | Information & communication | 2007 |
| Andrew Carr Engineering Ltd | Professional & scientific | 2026 |
| Andrew Scott Construction Ltd | Construction | 2024 |
| Angelos Athanas Ltd | Health & social care | 2025 |
| Angraflat Development Company Limited | Construction | 2016 |
| APS Industrial Doors Limited | Manufacturing | 2017 |
| Aptek (Scotland) Group Limited | Financial services | 2012 |
| Aptek (Scotland) Limited | Information & communication | 2006 |
| AR Transport (Kelso) Limited | Transport & logistics | 2017 |
| Archerfield Estates Ltd | Agriculture & fishing | 1987 |
| ARL Farming Ltd | Extraterritorial | 2012 |
| Asper Systems Limited | Manufacturing | 2004 |
| Atholl Estates Ltd | Real estate | 2017 |
| Attia Awais Limited | Wholesale & retail | 2025 |
| Aureus Business Consulting Limited | Professional & scientific | 2011 |
| B B TAN Investments Ltd. | Real estate | 2022 |
| B. Murray Limited | Other services | 2019 |
| B. RAE Contracting Limited | Administrative & support | 2005 |
| B. RAE Farming Ltd | Agriculture & fishing | 2015 |
| Baillie Project Management Ltd | Construction | 2012 |
| BAX Imaging Ltd | Professional & scientific | 2024 |
| Beck Farming Ltd | Agriculture & fishing | 2018 |
| Bielgrange Ltd | Agriculture & fishing | 2018 |
| Billabong Group Limited | Real estate | 2025 |
| Bioval Ltd | Professional & scientific | 2012 |
| Bisset Contractors Limited | Professional & scientific | 2025 |
| Blacklock & Douglas Limited | Construction | 2012 |
| Blakelaw Beef Farming Ltd | Agriculture & fishing | 2022 |
| Blendology Labs Limited | Manufacturing | 2025 |
| Border CAB Co Ltd | Transport & logistics | 2018 |
| Border Hardware Ltd | Wholesale & retail | 2011 |
| Border Hideaways Limited | Hospitality | 2024 |
| Border Installs Ltd | Administrative & support | 2025 |
| Border Lawns Limited | Administrative & support | 2024 |
| Border Livestock Exchange Limited | Wholesale & retail | 1995 |
| Border Padel Company Ltd | Wholesale & retail | 2025 |
| Border Render Ltd | Construction | 2022 |
| Border Stove and Chimney Services Ltd | Construction | 2022 |
| Border Union Agricultural Society | Administrative & support | 2001 |
| Borderline Athletics Ltd | Arts & recreation | 2023 |
| Borders Cookware Limited | Wholesale & retail | 2014 |
| Borders Joinery Conservation Ltd | Construction | 2018 |
| Borders Legal Holdings Ltd | Financial services | 2024 |
| Borders Legal Investments Ltd | Real estate | 2022 |
| Borders Legal Limited | Professional & scientific | 2017 |
| Borders Plant & Machinery Services Limited | Construction | 2020 |
| Borders School of Music Ltd | Education | 2022 |
| Borders Software Ltd | Professional & scientific | 2024 |
| Borders Surplus Store Ltd | Wholesale & retail | 2019 |
| Borders Yard Limited | Real estate | 2025 |
| Borland GAS Care Limited | Construction | 2016 |
| Bova-Gen Limited | Agriculture & fishing | 2003 |
| Bowhouse Properties Limited | Real estate | 2004 |
| Bowmont Farming Limited | Agriculture & fishing | 2017 |
| Bryan Moffat Limited | Construction | 2005 |
| Bryce of Scotland (Fencing) Limited | Manufacturing | 1986 |
| Budissa BAG (UK & Ireland) Ltd | Agriculture & fishing | 2022 |
| Burnside Bothies Ltd. | Hospitality | 2023 |
| Business Sale Basecamp Ltd | Information & communication | 2001 |
| Butterwell Developments Ltd | Construction | 2021 |
| Buy Design Gallery Limited | Wholesale & retail | 2008 |
| C & M Symons Ltd | Agriculture & fishing | 2004 |
| C & R Tyres Limited | Wholesale & retail | 1995 |
| C. M. Twemlon and Partners | | 1988 |
| C2 Asbestos Limited | Professional & scientific | 2025 |
| Caffe + (Galashiels) Ltd. | Hospitality | 2005 |
| Caledonian Cattle Company Limited | Extraterritorial | 2006 |
| Calidad Associates Limited | Professional & scientific | 2018 |
| Capelli Kelso Ltd | Other services | 2018 |
| Capelli Sun Ltd | Other services | 2024 |
| Cardney Limited | Professional & scientific | 2003 |
| Castlegate Nursery & OUT of School Club Limited | Other services | 2019 |
| Celui TAN Ltd | Wholesale & retail | 2022 |
| Central GAS Limited | Construction | 2016 |
| Central Guesthouse LLP | | 2016 |
| Cerebro AI Holdings Limited | Information & communication | 2025 |