| E&L Electrical Services Limited | Energy | 2025 |
| Eagle Estates Scotland Ltd | Real estate | 2017 |
| Eagles Retail Ltd | Wholesale & retail | 2024 |
| Easemee Cleanings Ltd | Administrative & support | 2026 |
| Easemee J Cleanings Ltd | Administrative & support | 2026 |
| Eclipse Hair & Beauty (Johnstone) Limited | Other services | 2009 |
| Edward Kevin Mccaffery Ltd | Households | 2021 |
| Effe MCC Ltd | Arts & recreation | 2025 |
| Elamari Foods Limited | Wholesale & retail | 2025 |
| Elcie’S Catering Ltd | Hospitality | 2019 |
| Elderslie Golf Club Limited | Arts & recreation | 2024 |
| Elemes Property Ltd | Real estate | 2026 |
| Elevate Scot Limited | Wholesale & retail | 2025 |
| Elite Towing Services Scotland Limited | Transport & logistics | 2020 |
| Elliott Property Scotland Ltd | Construction | 2012 |
| Elliston Design Ltd | Arts & recreation | 2013 |
| Elliston Ground Maintenance Limited | Administrative & support | 2024 |
| Emmerson Homes Limited | Real estate | 2016 |
| Empire Home Renovations Limited | Construction | 2025 |
| Energy Distribution Limited | Construction | 2015 |
| Energy PIG Installation Group Ltd | Construction | 2026 |
| Energy PIG Renewables Ltd | Construction | 2025 |
| Ennisbay Limited | Real estate | 1998 |
| Enterprise Occupational Health Ltd | Health & social care | 2014 |
| Equity Educational Psychology Services Ltd | Education | 2024 |
| Ethnic Construction Skills Development C.I.C. | Education | 2024 |
| Etopat Consultancy Services Limited | Information & communication | 2023 |
| Euroclean Commercial Cleaning Ltd | Administrative & support | 2014 |
| European GD Network Limited | Health & social care | 2016 |
| European Rare Diseases Network Limited | Health & social care | 2021 |
| EV Bay Solutions Ltd | Manufacturing | 2025 |
| Evaliam 1976 Limited | Real estate | 2018 |
| Ewan Bell Aviation Ltd | Professional & scientific | 2025 |
| Excel AI Ltd | Information & communication | 2026 |
| Experiential Learning Tools Ltd. | Information & communication | 2022 |
| Extraordinary Designs Ltd | Professional & scientific | 2024 |
| Extraordinary Developments Ltd | Real estate | 2025 |
| Extraordinary Show Homes Ltd | Real estate | 2024 |
| EZ Buy 2DAY Limited | Wholesale & retail | 2026 |
| Fair Pley Limited | Arts & recreation | 2003 |
| Fallow Fresh Ltd | Wholesale & retail | 2025 |
| FDO Properties Scotland Limited | Real estate | 2024 |
| Fencetec Ltd | Administrative & support | 2019 |
| First Concert Agency Ltd | Professional & scientific | 2023 |
| Fitness Inferno Limited | Arts & recreation | 2022 |
| FIVE9TESTING.COM Limited | Information & communication | 2024 |
| Flacton Ltd | Transport & logistics | 2018 |
| Flexible First AID Training Ltd | Health & social care | 2013 |
| Floorman Limited | Administrative & support | 2012 |
| Florida Select Villas Ltd | Real estate | 2008 |
| Fmtt Service Limited | Administrative & support | 2017 |
| Fonology Ltd | Wholesale & retail | 2020 |
| Fons ET Origo Limited | Hospitality | 2024 |
| Football Buzz West Ltd | Arts & recreation | 2024 |
| Football Foundry Ltd | Education | 2026 |
| Forbes Construction (Scotland) Ltd | Construction | 2018 |
| Fortie Consulting Limited | Professional & scientific | 2013 |
| Fortn Ltd | Information & communication | 2022 |
| Forward Management Consulting Limited | Professional & scientific | 2018 |
| Four Seasons Real Estates Limited | Real estate | 2016 |
| Foys Formulas Ltd | Professional & scientific | 2025 |
| Fuelstar Ltd | Wholesale & retail | 2025 |
| G B Property Maintenance (Scotland) Limited | Construction | 2005 |
| G.R.F Plant Ltd | Construction | 2023 |
| G12 Properties Limited | Real estate | 2024 |
| G15 Properties Limited | Real estate | 2019 |
| G7 Property Ltd | Real estate | 2022 |
| Galhal Ltd | Agriculture & fishing | 2020 |
| Gallagher Hospitality Ltd | Hospitality | 2024 |
| Gasfit Plumbing & Heating Ltd | Construction | 2018 |
| Gatley Holdings Ltd | Real estate | 2020 |
| GEE MAC Autos Ltd | Wholesale & retail | 2020 |
| Gemmel Rentals Ltd | Real estate | 2024 |
| Geomats Ltd | Construction | 2025 |
| Giemar Engineering Ltd | Construction | 2014 |
| Gilmour Property Services Ltd | Administrative & support | 2015 |
| Ginger Root Contracts Limited | Construction | 2014 |
| GK Optometry Ltd | Administrative & support | 2017 |
| Glasgow Airport Taxi Services Ltd | Transport & logistics | 2015 |
| Glasgow Airport Taxis Ltd | Transport & logistics | 2020 |
| Glenbell 1 Limited | Real estate | 2012 |
| Glenbell 2 Limited | Professional & scientific | 2012 |
| GMC Decor Limited | Construction | 2024 |
| GMG Autos Ltd | Wholesale & retail | 2013 |
| GNG Interiors Ltd | Construction | 2025 |
| GNT Traders Ltd | Wholesale & retail | 2025 |
| Golden Star Ltd | Hospitality | 2026 |
| Goldie Solutions Ltd | Information & communication | 2024 |
| Golf in Scotland Limited | Administrative & support | 2022 |
| Gordonfrasercars Limited | Wholesale & retail | 2016 |
| Gorman Uplifts Ltd | Water & waste | 2024 |
| Gowanlea Garden Centre Ltd | Wholesale & retail | 2022 |
| Gracen Holdings Limited | Real estate | 2016 |
| Graeme Lawson Ltd | Information & communication | 2019 |
| Graham Cook (US) Limited | Professional & scientific | 2019 |
| Grants' School of Music and Performance Ltd | Education | 2020 |
| Gravity Robotics and Automation Limited | Manufacturing | 2024 |
| Gray Sparrow Group Ltd | Construction | 2024 |
| GRB Constuction and Installation Limited | Construction | 2018 |
| Great Circle Training Ltd | Professional & scientific | 2019 |