| A & L Marketplace Ltd | Wholesale & retail | 2026 |
| A. E. Riding Limited | Agriculture & fishing | 2016 |
| Abbey Bitters Limited | Manufacturing | 2026 |
| Abbey Bridge Coffee House Ltd | Hospitality | 2021 |
| Abosie Architects Limited | Professional & scientific | 2022 |
| Alaa Limited | Wholesale & retail | 2024 |
| Allerton House (Jedburgh) Ltd | Hospitality | 2023 |
| Amirrah Enterprises Ltd | Wholesale & retail | 2025 |
| Amos Property Ltd | Real estate | 2024 |
| Ancrum Public Hall | Other services | 2004 |
| Antony Field Builder Ltd | Construction | 2025 |
| Apostrophe Woodcraft Limited | Manufacturing | 2006 |
| Armstrong Construction and Property Services Limited | Construction | 2017 |
| Artaffect Studios and Gallery Ltd | Arts & recreation | 2025 |
| Autistic Associates Ltd | Information & communication | 2025 |
| Ballaro Ltd | Hospitality | 2023 |
| Bathgate & Cornwell Limited | Real estate | 2024 |
| Birkenside Estates Limited | Hospitality | 2022 |
| Black Swan Trading Ltd | Information & communication | 2016 |
| Blackshaw Management Consulting Limited | Professional & scientific | 2010 |
| Blue Skies Limited | Hospitality | 2008 |
| Boni Investments Ltd | Real estate | 2026 |
| Bonnie Borders Ltd | Hospitality | 2024 |
| Border Arts Development C.I.C. | Arts & recreation | 2016 |
| Border Building Solutions Ltd | Construction | 2025 |
| Border Demolition Limited | Construction | 2020 |
| Border Eco Systems Limited | Construction | 2009 |
| Border Global Travel Ltd | Administrative & support | 2016 |
| Border Property Maintenance Ltd | Extraterritorial | 2008 |
| Border Road Planings Ltd | Administrative & support | 2019 |
| Borders Accounting & Admin Solutions Ltd | Professional & scientific | 2024 |
| Borders Alternative Addictions CIC | Health & social care | 2019 |
| Borders and Lodging Ltd | Hospitality | 2025 |
| Borders Blasting Ltd | Manufacturing | 2023 |
| Born in Scotland Buchanan Galleries Ltd | Wholesale & retail | 2021 |
| Born in Scotland East Kilbride Ltd | Wholesale & retail | 2021 |
| Born in Scotland Edinburgh Gyle Ltd | Wholesale & retail | 2021 |
| Born in Scotland Livingston Ltd | Wholesale & retail | 2021 |
| Born in Scotland Ltd | Financial services | 2020 |
| Born in Scotland ST Andrews Ltd | Wholesale & retail | 2026 |
| Born in Scotland ST Enoch Ltd | Wholesale & retail | 2020 |
| Born in Scotland Stirling Thistles Ltd | Wholesale & retail | 2020 |
| Born in Scotland Waverley Mall Ltd | Wholesale & retail | 2020 |
| Brewster Home Services Ltd | Administrative & support | 2024 |
| Bullseye Stump Removal Ltd. | Agriculture & fishing | 2007 |
| Cairnmount Properties Ltd | Real estate | 2020 |
| Canmore Investments | | 1992 |
| CCR Agri Services Ltd | Agriculture & fishing | 2021 |
| CCS Cleaning Services Ltd | Administrative & support | 2019 |
| Chesters Stables Ltd | Hospitality | 2009 |
| Cindy Webster Cooks Limited | Administrative & support | 2010 |
| CJA Cleaning Ltd | Administrative & support | 2022 |
| Clamp&Co Ltd | Real estate | 2024 |
| Clanland Ltd | Wholesale & retail | 2011 |
| Clark & Weir Limited | Agriculture & fishing | 2003 |
| CLB Inspection Ltd | Other services | 2025 |
| Clearwater Dental Ltd | Health & social care | 2021 |
| Colin Yourston & SON Limited | Construction | 2010 |
| Crailing Enterprises Limited | Wholesale & retail | 2002 |
| Crailing Nook Farm Partnership | | 1997 |
| Crosskeys Property Limited | Real estate | 2026 |
| CRS Contracting Ltd | Construction | 2020 |
| Cruickshank and Partners | | 1980 |
| D Gobby Builders Ltd | Construction | 2017 |
| Debelas Ltd. | Construction | 2016 |
| Elevation North Ltd | Wholesale & retail | 2021 |
| Equine Physiotherapy UK Ltd | Professional & scientific | 2022 |
| Finlayson Automotive Ltd | Wholesale & retail | 2011 |
| Folk Studio Limited | Information & communication | 2023 |
| G & J Groundworks(Jedburgh) Ltd | Construction | 2017 |
| G D Shiell and SON | | 1978 |
| George & Grieve Joinery Ltd | Construction | 2014 |
| Gibson & Hall Ltd. | Construction | 2007 |
| Gilbert Developments Limited | Construction | 1988 |
| Gilbert Investments (Scotland) Limited | Real estate | 2004 |
| Gilbert Mcclung (Kelso) Limited | Construction | 2011 |
| Glebe Forestry & Rural Ltd | Agriculture & fishing | 2026 |
| Glenteviot Farmers (Ruecastle) | | 1979 |
| Gmagile Ltd | Information & communication | 2025 |
| Golden Measure Limited | Manufacturing | 2016 |
| Golden River Jedburgh Limited | Hospitality | 2023 |
| Gordon Contracts Ltd | Administrative & support | 2014 |
| Graham Macdonald (Ancrum) Limited | Construction | 2000 |
| Grahams Installations Ltd | Construction | 2025 |
| Greentweed Eco CIC | Professional & scientific | 2023 |
| H&R Automotive Ltd | Wholesale & retail | 2026 |
| Hagb Ltd | Wholesale & retail | 2020 |
| Hallmark Access Goods Ltd | Wholesale & retail | 2025 |
| Hallowgate Studios Ltd | Information & communication | 2026 |
| Handcrafted at Rowandene Limited | Manufacturing | 2024 |
| Hanlon Groundworks Limited | Construction | 2016 |
| Henderson Firewood Ltd | Wholesale & retail | 2025 |
| Herron Homes Ltd | Real estate | 2025 |
| Horse Mate Community Interest Company | Agriculture & fishing | 2025 |
| IFC Inspection Limited | Professional & scientific | 2022 |
| Industrial Nature Ltd | Manufacturing | 2016 |
| Industrial Nature UK Ltd | Manufacturing | 2020 |
| Ittude Limited | Information & communication | 2024 |
| J. H. Johnson and Sons | | 1978 |
| Jacksons at Jedburgh Ltd | Arts & recreation | 2025 |