| A J Robson Marine Ltd | Administrative & support | 2017 |
| Abbey Court Flats Management Company Limited | Households | 2003 |
| Atlas Construction Plant Services Limited | Construction | 2025 |
| Bryher Boatyard Limited | Manufacturing | 2011 |
| Bryher Campsite Ltd | Hospitality | 2015 |
| Burnt Island Ltd | Hospitality | 2026 |
| C.J Stone Works Ltd | Construction | 2021 |
| Calyn Gardens Ltd | Administrative & support | 2023 |
| Carnwethers Ltd | Hospitality | 2017 |
| Castle Farm House Ltd | Real estate | 2023 |
| Castle Farm Shop Ltd | Wholesale & retail | 2024 |
| Community Infrastructure Scilly C.I.C. | Energy | 2023 |
| Cosmos Community Observatory ST Martins on Scilly Community Interest Company | Professional & scientific | 2018 |
| Daymark Foods Ltd | Wholesale & retail | 2026 |
| Falcon Boating Ltd | Transport & logistics | 2003 |
| Field House (Isles of Scilly) Management Company Limited | Households | 1991 |
| Five Island Trading Limited | Wholesale & retail | 2016 |
| Golden Bay Mansions Limited | Hospitality | 1962 |
| Harbour Side Cafe IOS Ltd | Hospitality | 2026 |
| Hillside Farm Bryher Ltd | Agriculture & fishing | 2025 |
| Hugh Street Cafe Limited | Hospitality | 2019 |
| HUT 62 Tresco Ltd | Administrative & support | 2025 |
| IOS Boat Co Ltd | Manufacturing | 2018 |
| Island Fish Limited | Agriculture & fishing | 2015 |
| Island Marine Services Scilly Ltd | Transport & logistics | 2025 |
| Island Outboard Services (IOS) Ltd | Manufacturing | 2023 |
| Island Tiger Limited | Agriculture & fishing | 2007 |
| Isles of Scilly Community Venture C.I.C. | Energy | 2017 |
| Isles of Scilly Steamship Company Limited | Transport & logistics | 1920 |
| Isles of Scilly Wildlife Trust Limited | Education | 2003 |
| Johnson Building Consultancy Limited | Professional & scientific | 2021 |
| Lantern Taverns Ltd | Hospitality | 2022 |
| Longitude & Latitude Limited | Professional & scientific | 2000 |
| Longstone Lodge and Cafe Ltd | Hospitality | 2016 |
| Moonrakers Management Limited | Real estate | 2011 |
| O J Love Ltd | Wholesale & retail | 2025 |
| Ocealis Ltd | Information & communication | 2019 |
| P R Gilbert Plumbing and Heating Limited | Construction | 2016 |
| Paulger's Passenger Transport Limited | Transport & logistics | 2003 |
| Penlee House Scilly Ltd | Households | 2020 |
| Porthcressa Limited | Wholesale & retail | 2003 |
| Porthennor Pubs Ltd | Hospitality | 2022 |
| Sandpiper Shop (Scilly) Limited(The) | Wholesale & retail | 1977 |
| Schooners Hotel (IOS) Ltd | Hospitality | 2025 |
| Schwarz & Co Ltd | Health & social care | 2011 |
| Scillonia Boatshare Limited | Transport & logistics | 2009 |
| Scillonian Holdings Ltd. | Real estate | 1983 |
| Scillonian Marine Consultants Limited | Professional & scientific | 2016 |
| Scillonian Properties Ltd | Real estate | 1983 |
| Scillonian Trading Limited | Construction | 2015 |
| Scilly Carts Limited | Administrative & support | 2021 |
| Scilly Coastal Clean-Up CIC | Water & waste | 2025 |
| Scilly Estate Agents Ltd | Real estate | 2026 |
| Scylan Limited | Wholesale & retail | 2025 |
| Sdltrades Limited | Information & communication | 2025 |
| Seaquest Scilly Ltd | Transport & logistics | 2025 |
| Shadow Marine Ltd | Manufacturing | 2024 |
| Shearwater Construction Limited | Construction | 2015 |
| Somnium Vita Ltd | Hospitality | 2022 |
| ST Mary's Mews (Isles of Scilly) Management Company Limited | Households | 2024 |
| Steve Oates Limited | Manufacturing | 2019 |
| THE Foredeck IOS Ltd | Wholesale & retail | 2021 |
| THE Isles of Scilly Boat Hire Company Ltd | Extraterritorial | 2016 |
| THE Sailing Centre, Isles of Scilly Limited | Arts & recreation | 2001 |
| THE Wrasse (Isles of Scilly) Limited | Households | 1990 |
| Tth@Ios Limited | Hospitality | 2016 |
| Two Boys IOS Ltd | Agriculture & fishing | 2025 |
| V6 Performance Limited | Wholesale & retail | 2023 |
| VG Independent Consultancy Limited | Health & social care | 2026 |
| Warleggan Management Ltd | Real estate | 2016 |
| Wheelhouse Guest House Limited | Hospitality | 2010 |
| Woodcock and Mumford Limited | Real estate | 1951 |
| World Pilot GIG Championships CIC | Administrative & support | 2025 |