| 2F SIM Events Limited | Arts & recreation | 2024 |
| AC Subsea Ltd | Professional & scientific | 2020 |
| ACC Construction Islay Ltd | Construction | 2023 |
| Agri Services Islay Ltd | Agriculture & fishing | 2016 |
| Aitchison Ltd | Construction | 2010 |
| Aitken Shellfish Limited | Agriculture & fishing | 2009 |
| AJ Cunningham Ltd | Construction | 2023 |
| Alex Birse Haulage Ltd | Transport & logistics | 2021 |
| Altru Construction Ltd | Construction | 2025 |
| AN Lanndaidh Publishing Ltd | Arts & recreation | 2021 |
| Anam Cara Properties Ltd | Real estate | 2025 |
| Another Place the Machrie Limited | Hospitality | 2015 |
| Anvil Art Engineering Limited | Manufacturing | 2013 |
| Ardtalla Estates Limited | Agriculture & fishing | 1973 |
| Aromatics of the Hebrides Ltd | Manufacturing | 2019 |
| BL Contracts Limited | Construction | 2009 |
| Blas MOR Homes Ltd | Construction | 2025 |
| Bridgend Shop Limited | Real estate | 2014 |
| C Brodie Limited | Wholesale & retail | 2011 |
| C, C & L Shellfish Ltd | Agriculture & fishing | 2018 |
| Caiber Fishing Co Ltd | Agriculture & fishing | 2025 |
| Campbells of Islay Ltd | Wholesale & retail | 2019 |
| Carnain Farm 1985 Partnership | | 1989 |
| Coille Farm Partnership | | 1997 |
| Colin Logan Building Contractors Ltd | Construction | 2009 |
| Cragabus House Ltd | Real estate | 2026 |
| Craigard Ltd | Hospitality | 2019 |
| Cresswell Cars Ltd | Manufacturing | 2017 |
| D Drennan Maintenance and Fabrication Limited | Construction | 2023 |
| Excavation Scotland Ltd | Construction | 2026 |
| G & C Mccormick & Sons Ltd | Transport & logistics | 2026 |
| Glenegedale Islay Ltd | Hospitality | 2017 |
| GMT Contracts Ltd | Construction | 2026 |
| Heather's Ices Isle of Islay Limited | Manufacturing | 2022 |
| Iain Maccuaig Property Maintenance Limited | Construction | 2004 |
| Iain Woodrow Electrical Contractor Limited | Construction | 2017 |
| IFH Ventures Limited | Agriculture & fishing | 2020 |
| Iomairt Chille-Chomain | Arts & recreation | 2001 |
| Ionad Chaluim Chille ILE | Education | 2000 |
| Islay & Jura Community Enterprises Limited | Arts & recreation | 1989 |
| Islay & Jura Ferry Company Limited | Transport & logistics | 1988 |
| Islay Ales Company Limited | Manufacturing | 2003 |
| Islay Auto Services Ltd | Wholesale & retail | 2019 |
| Islay Cabins Limited | Hospitality | 2020 |
| Islay Car Hire Limited | Manufacturing | 2023 |
| Islay Catering Ltd. | Hospitality | 2017 |
| Islay Designs Limited | Wholesale & retail | 2003 |
| Islay Development Initiative Ltd | Agriculture & fishing | 2003 |
| Islay Events Ltd | Hospitality | 2024 |
| Islay Ferry Company Limited | Transport & logistics | 2012 |
| Islay Games Ltd | Information & communication | 2014 |
| Islay GIN Ltd | Manufacturing | 2017 |
| Islay Home Stores Limited | Wholesale & retail | 2019 |
| Islay Minibus & Taxi Hire Limited | Transport & logistics | 2009 |
| Islay Sea Adventures Ltd | Arts & recreation | 2015 |
| Islay Seasons Ltd | Professional & scientific | 2026 |
| Islay Spirit Apparel Ltd | Wholesale & retail | 2026 |
| Islay Transport Limited | Transport & logistics | 2022 |
| Islay Whisky Connection Ltd | Hospitality | 2019 |
| Islay, Jura & Colonsay Agricultural Association | Administrative & support | 2009 |
| Islesman Ltd | Real estate | 2013 |
| JMF Engineering & Renewables Ltd | Professional & scientific | 2019 |
| Jukebox GIN Ltd | Manufacturing | 2020 |
| Kevin Heads Transport Limited | Transport & logistics | 2023 |
| Kilchoman Community Park Limited | Hospitality | 2009 |
| Kilchoman Distillery Company Ltd. | Manufacturing | 2001 |
| Kildalton Estate Ltd | Real estate | 2015 |
| Kimbiekoo Ltd | Hospitality | 2025 |
| KVD Services Limited | Agriculture & fishing | 2016 |
| Laggan Landscaping Ltd | Professional & scientific | 2023 |
| Lamb Taxi Ltd | Transport & logistics | 2026 |
| Lenimore Limited | Information & communication | 2020 |
| LEO and TED Ltd | Hospitality | 2014 |
| Leorin Farming Company | | 1990 |
| Leorin Limited | Real estate | 2022 |
| Lochside Hotel Trading Limited | Hospitality | 2020 |
| Mactaggart Youth and Communities Outreach Service | Public administration | 1998 |
| Mansefield JG Limited | Real estate | 2016 |
| Martyn Chandler Admin Ltd | Administrative & support | 2025 |
| Miramar Catering Ltd | Hospitality | 2025 |
| Neocirrus Limited | Professional & scientific | 2023 |
| Nerabus Market Garden Ltd | Agriculture & fishing | 2020 |
| NO1 Charlotte Street Limited | Hospitality | 2019 |
| North Western Trawlers Limited | Agriculture & fishing | 2012 |
| NRM Plumbing Ltd | Construction | 2023 |
| Original Impspeed Limited | Extraterritorial | 2003 |
| Orsay Sea Salt Ltd | Manufacturing | 2020 |
| Orsay Seafood Ltd | Wholesale & retail | 2024 |
| Overland Route Company Limited | Transport & logistics | 1991 |
| P Macallister Groundworks Ltd | Construction | 2017 |
| Port Ellen Laundry Services Ltd | Other services | 2020 |
| Port Ellen Power Ltd | Construction | 2026 |
| R Basnett Carpentry and Joinery Ltd | Manufacturing | 2025 |
| Reynier Research Limited | Professional & scientific | 2005 |
| RFH Consulting Ltd | Professional & scientific | 2023 |
| Rhinns Smokehouse Ltd | Manufacturing | 2025 |
| Rockside Farming Co Limited | Agriculture & fishing | 1997 |
| Salty Gull Limited | Wholesale & retail | 2023 |
| Scott Hastie Ltd | Construction | 2024 |
| Scouler Ltd | Real estate | 2020 |