| Rainbow Candy Ltd | Wholesale & retail | 2021 |
| Rainey Mckechnie Podiatry Ltd | Health & social care | 2025 |
| RAS Group Limited | Construction | 2016 |
| RAS Technical Services Limited | Construction | 2020 |
| Ravenmoy Ltd | Manufacturing | 2025 |
| RBD Property Limited | Real estate | 2019 |
| RBM Lubrication & Monitoring Solutions Ltd. | Manufacturing | 2011 |
| RBM Maintenance Solutions Ltd | Professional & scientific | 2024 |
| Reids Carpet Outlet Ltd | Wholesale & retail | 2010 |
| Restructa Holdings Limited | Financial services | 2021 |
| Restructa LCD Ltd | Other services | 2022 |
| Restructa Ltd. | Wholesale & retail | 2005 |
| Riddex & Co Property Ltd | Real estate | 2024 |
| Rigworx Construction Ltd | Construction | 2024 |
| River-Gate Gifts Limited | Wholesale & retail | 2025 |
| Riverview Luxury Lodges Ltd | Real estate | 2022 |
| Rkcs Rail Limited | Construction | 2015 |
| RM Construction (Ayrshire) Ltd | Construction | 2022 |
| RM Plant Hire Ltd | Wholesale & retail | 2025 |
| Rmbsurveys Ltd | Professional & scientific | 2025 |
| RNC Consult Ltd | Construction | 2026 |
| RNS Properties Ltd | Real estate | 2022 |
| Robert Paterson Ltd | Manufacturing | 2017 |
| Robert Wilson & SON Ltd | Administrative & support | 2025 |
| Robison Joinery Ltd | Construction | 2024 |
| Rocco 89 Ltd | Real estate | 2019 |
| Rope Access Services Ayrshire Ltd | Mining & quarrying | 2022 |
| Rose James Wilson Limited | Arts & recreation | 2025 |
| Rosstel Ltd | Professional & scientific | 2023 |
| Rosstoration Surveys Ltd | Construction | 2013 |
| Route 300 Campers Ltd | Manufacturing | 2024 |
| RP Engineering Services Limited | Professional & scientific | 2023 |
| RS GAS Solutions Ltd | Construction | 2023 |
| RUA Biomaterials Limited | Extraterritorial | 1988 |
| RUA Life Sciences PLC | Manufacturing | 1996 |
| RUA Medical Devices Limited | Manufacturing | 2011 |
| RUA Structural Heart Limited | Professional & scientific | 2011 |
| RUA Vascular Limited | Professional & scientific | 2015 |
| RZ Access Specialists Ltd | Construction | 2026 |
| S Johnson & SON Ltd | Administrative & support | 2023 |
| S Quinn Property Limited | Real estate | 2017 |
| S&S Plastering Glasgow Limited | Construction | 2017 |
| S.O Contract Solutions Limited | Administrative & support | 2017 |
| SAE Renewables Ltd | Construction | 2021 |
| SAE Solutions Ltd | Construction | 2018 |
| Safe Secure Storage Ltd | Transport & logistics | 2021 |
| Sahm Parts Ltd | Wholesale & retail | 2020 |
| Saif's Supermarket Limited | Wholesale & retail | 2020 |
| SAJ Property Services Ltd | Construction | 2023 |
| Sales & Survey Solutions Limited | Administrative & support | 2016 |
| Salieri's Bar Ltd | Hospitality | 2016 |
| Salon Masters Ltd | Professional & scientific | 2023 |
| Saltire Psychology Ltd | Education | 2025 |
| Sandyyabass Ltd | Wholesale & retail | 2025 |
| Sanmex International Limited | Wholesale & retail | 1982 |
| Sanmina-Sci UK Limited Partnership | | 2002 |
| Sassys Beauty School Ltd | Other services | 2021 |
| Saughtree Holdings Ltd | Financial services | 2017 |
| Save the Call Ltd | Information & communication | 2026 |
| SCD Joinery Ltd | Construction | 2023 |
| Scented Gifts UK Ltd | Wholesale & retail | 2024 |
| SCO012010 Ltd | Administrative & support | 2010 |
| Scot Baillie Joinery Ltd | Manufacturing | 2025 |
| Scotbok Properties Ltd | Real estate | 2022 |
| Scotia Energy Assessors Limited | Professional & scientific | 2015 |
| Scotia Freight Ltd | Transport & logistics | 2025 |
| Scotia Leisure Homes Ltd | Wholesale & retail | 2023 |
| Scotlee Commercials Limited | Wholesale & retail | 2015 |
| Scotlee Holdings Limited | Financial services | 2004 |
| Scotlee Limited | Real estate | 1998 |
| Scotlee Transport Services Limited | Transport & logistics | 1978 |
| Scott Cabs (Ayrshire) Ltd | Transport & logistics | 2024 |
| Scott Renovations Ltd | Construction | 2022 |
| Scottish Maritime Museum Trust | Arts & recreation | 1983 |
| Scrimsign (Micro-Electronics) Limited | Administrative & support | 1986 |
| Scruffy DUG Design Solutions Limited | Information & communication | 2016 |
| SDC Technologies Inc. | | 1995 |
| SDC Technologies, Inc. | | 2025 |
| Seagate Properties Ltd | Real estate | 2018 |
| Secrets of Success Trading AS BNI Scotland West Limited | Other services | 2025 |
| SET Financial Limited | Financial services | 2024 |
| SET Rezidential Limited | Real estate | 2025 |
| SET Sail Tattoo Studio Limited | Other services | 2021 |
| SG Joiners and Builders Ltd | Construction | 2023 |
| SG8 Retail Ltd | Administrative & support | 2017 |
| Shalomdaly Ltd | Health & social care | 2018 |
| Sharp Installations Ltd. | Construction | 2004 |
| SHE Elite USA Ltd | Education | 2026 |
| Shewalton Car Spares Limited | Water & waste | 2003 |
| Shewalton Recycling Company Limited | | 1994 |
| SHG Electrical Ltd | Professional & scientific | 2024 |
| Shire Windows & Doors Ltd | Construction | 2020 |
| Shire Windows Installations Ltd | Construction | 2022 |
| Shyglance WK Limited | Hospitality | 2023 |
| Siaara Ltd | Wholesale & retail | 2025 |
| Signature Works (Scotland) Limited | Hospitality | 2015 |
| Signs 2 Impress Ltd | Manufacturing | 2021 |
| SIM Properties (Scotland) Ltd | Real estate | 2024 |
| Simpson Developments (Ayrshire) Ltd | Administrative & support | 2016 |
| Sink OR Skim Ltd | Wholesale & retail | 2025 |