| UPM Wood Materials (UK) Limited | Administrative & support | 2017 |
| Upm-Kymmene (UK) Limited | Manufacturing | 1987 |
| V.V.V. Limited | Transport & logistics | 2022 |
| Valley Homes (Scotland) Limited | Construction | 2017 |
| Valley Rooms Ayrshire Ltd | Hospitality | 2026 |
| Vape Ayrshire Ltd | Wholesale & retail | 2022 |
| Veinlode Ltd | Construction | 2026 |
| Velara Hsec Ltd | Information & communication | 2026 |
| Veritas Quality Management Ltd | Professional & scientific | 2023 |
| Vertex Goods Ltd | Wholesale & retail | 2026 |
| Vesfur Ltd | Wholesale & retail | 2023 |
| Village Centre Store Ltd | Wholesale & retail | 2013 |
| Village Homes Irvine Limited | Construction | 2021 |
| Visualize This Limited | Arts & recreation | 2012 |
| VLM Property Rentals Ltd | Real estate | 2025 |
| VMC Transport Ltd | Other services | 2018 |
| VVV Property Limited | Real estate | 2025 |
| W. Speirs & Sons Limited | Manufacturing | 2011 |
| Waddell Decor Ltd | Other services | 2018 |
| Walcal Property Development Limited | Administrative & support | 2000 |
| Walker Cladding Limited | Construction | 2013 |
| Wall Finishes Ltd | Construction | 2015 |
| WC Aero Ltd | Manufacturing | 2023 |
| WE ARE Nature CIC | Professional & scientific | 2025 |
| Weddings by Carlo Ltd | Professional & scientific | 2020 |
| WEE Street Ltd | Arts & recreation | 2025 |
| Weir Traffic Safety Ltd | Professional & scientific | 2019 |
| West Coast Campers Ltd | Administrative & support | 2025 |
| West Coast Fixing Limited | Construction | 2017 |
| West Coast Landscaping and Groundworks Ltd | Administrative & support | 2024 |
| West Coast Pallets Ltd. | Manufacturing | 2010 |
| West Coast Pubs (Ayrshire) Ltd. | Hospitality | 2009 |
| West Coast Pubs (Kilmarnock) Ltd. | Hospitality | 2013 |
| Westcoast Sheds (Ayrshire) Ltd | Construction | 2024 |
| Westscot Groundcare Ltd | Administrative & support | 2018 |
| Westwinds Beauty Retreat Ltd | Other services | 2025 |
| Wheretoglasgow Limited | Information & communication | 2019 |
| Whitehirst Kilwinning Limited | Construction | 2016 |
| Whyte's Property Company Ltd | Real estate | 2018 |
| Wicked Costumes Group Limited | Professional & scientific | 2011 |
| Wicked Costumes Holdings Limited | Professional & scientific | 2022 |
| Wicked Costumes Limited | Arts & recreation | 2008 |
| Wicked Costumes Trading Limited | Extraterritorial | 2011 |
| Wild Spa Scotland Ltd | Health & social care | 2024 |
| William Carson (Plumbing & Heating) Limited | Construction | 2016 |
| William Powell Engineer Ltd | Manufacturing | 2016 |
| Wilson Automotive Services Ltd | Administrative & support | 2016 |
| Wisa Plywood Limited | Administrative & support | 2025 |
| Wolf and Stag Media Limited | Extraterritorial | 2015 |
| Woodmill Limited | Construction | 2016 |
| Woodsmith Joiners Ltd | Construction | 2021 |
| Woof Studios Limited | Hospitality | 2023 |
| Wright Rope Services Ltd | Construction | 2024 |
| Xlnt Vitamins Ltd | Wholesale & retail | 2023 |
| XO Rigging & Scaffolding Limited | Construction | 2023 |
| XS Irvine Limited | Wholesale & retail | 2008 |
| XS Property Limited | Real estate | 2008 |
| Xs-Stock.Com Limited | Wholesale & retail | 2008 |
| Yarding Property Ltd. | Real estate | 2009 |
| Younger Play Limited | Construction | 2022 |
| Your Location Limited | Professional & scientific | 2013 |
| Your Options Understood CIC | Education | 2015 |
| Z&R Trade Ltd | Wholesale & retail | 2018 |
| Zisys Avmn Ltd. | Information & communication | 2002 |
| ZJ Rope Access Ltd | Construction | 2023 |