| 1G Project Services Limited | Administrative & support | 2017 |
| 1ST Architects (Scotland) Ltd | Professional & scientific | 2019 |
| AB Vennel Ltd | Other services | 2017 |
| Abbott Trading Ltd. | Construction | 2024 |
| ABS Rail Services Limited | Administrative & support | 2017 |
| ADS (Alba Digital Solutions) LLP | | 2020 |
| ADS Ventures Ltd | Wholesale & retail | 2024 |
| Advanced Stairlifts (Scotland) Limited | Administrative & support | 1997 |
| AIC Heating UK Limited | Wholesale & retail | 2018 |
| AJ Private Ltd | Wholesale & retail | 2025 |
| AKW Construction and Shop Fitting Ltd | Construction | 2025 |
| Albert Thain Holdings Limited | Professional & scientific | 1963 |
| Albert Thain Limited | Professional & scientific | 1937 |
| Aldermere Commerce Ltd | Wholesale & retail | 2026 |
| Aliathon Limited | Information & communication | 2001 |
| Alpha Blinds (INB) Ltd | Construction | 2011 |
| Apex Design & Build Ltd | Construction | 2019 |
| Apex Drone Imaging Ltd | Professional & scientific | 2026 |
| Arlmar Maritime Services Ltd | Professional & scientific | 2024 |
| Artivoo Ltd | Information & communication | 2024 |
| ASG Plumbing and Heating Limited | Construction | 2018 |
| Asgb Limited | Water & waste | 2016 |
| Authcode Limited | Wholesale & retail | 2018 |
| Autimize AI Ltd | Information & communication | 2025 |
| Axile Ltd | Information & communication | 2019 |
| B G Vent Limited | Manufacturing | 2000 |
| Bass Marine Ltd | Construction | 2026 |
| Bayview Fife Ltd | Real estate | 2015 |
| BB Joinery & Construction Ltd | Construction | 2024 |
| BB Joinery Fife Ltd | Construction | 2026 |
| BBM 1 Limited | Construction | 2019 |
| Belfast Celtic Football & Athletic Company, Limited | Arts & recreation | 2018 |
| Beloved! Group Ltd | Hospitality | 2025 |
| Bethel Communications Ltd | Education | 2014 |
| Better Space Properties Ltd | Real estate | 2025 |
| BHH Property Limited | Real estate | 2016 |
| Blueecho Innovations Ltd | Wholesale & retail | 2025 |
| Bramble PM Consulting Ltd | Professional & scientific | 2025 |
| Cairnpark Property Ltd | Construction | 2020 |
| Cartmore Properties Limited | Real estate | 1991 |
| Catalyst Business (UK) Ltd | Other services | 2014 |
| Ceren Takeaway Ltd | Hospitality | 2018 |
| Christina Hamilton CRA Limited | Professional & scientific | 2015 |
| Coistie Construction Ltd | Construction | 2025 |
| Cornucopia EH11 Limited | Real estate | 2022 |
| Cozmosys Ltd | Hospitality | 2025 |
| CPC Construction Supplies Limited | Wholesale & retail | 2024 |
| Cpc-Mcb Ltd | Construction | 2020 |
| Crathes Consulting Ltd | Professional & scientific | 2025 |
| CRM Financial Planning Ltd | Professional & scientific | 2024 |
| Crownstone Digital Retail Ltd | Wholesale & retail | 2026 |
| CSR Private Limited | Wholesale & retail | 2025 |
| Cumbrae Data Consultancy and Training Ltd | Information & communication | 2020 |
| Cyclone Buildings Limited | Construction | 2019 |
| Cyclone Steel Buildings Limited | Construction | 2019 |
| Daily a Store Ltd | Wholesale & retail | 2026 |
| Deer Park Golf & Country Club Limited | Professional & scientific | 1988 |
| Denholm HAY Limited | Health & social care | 2012 |
| DKC Technical Consultancy Ltd | Professional & scientific | 2024 |
| Donibristle Skip Hire & Recycling Ltd | Administrative & support | 2017 |
| Dundee Decommissioning Limited | Water & waste | 2012 |
| Dutch Sign & Design Ltd | Professional & scientific | 2023 |
| E DEY Electrical Ltd | Construction | 2024 |
| E. DEY Electrical Services Ltd. | Manufacturing | 2026 |
| East Ness Pier Ltd | Agriculture & fishing | 2026 |
| EB Electrical Limited | Manufacturing | 1999 |
| Edinburgh Capital Tours Limited | Administrative & support | 2023 |
| Edinburgh Joiners Ltd | Construction | 2023 |
| Eiger Sport & Business Performance Ltd | Arts & recreation | 2014 |
| ESC Packaging Limited | Manufacturing | 2019 |
| Exel Private Hire Ltd | Transport & logistics | 2019 |
| FAB Cabs Limited | Professional & scientific | 2003 |
| Festival Travel (Scotland) Limited | Transport & logistics | 2004 |
| Fife Takeaways Ltd | Hospitality | 2025 |
| Fine Products Exporters Limited | Wholesale & retail | 2014 |
| Fior Anam Ltd | Professional & scientific | 2025 |
| Flemings Logistics Limited | Transport & logistics | 2021 |
| Fleur Properties Ltd | Real estate | 2019 |
| Forth Bridge Stevedoring Limited | Transport & logistics | 2000 |
| Forth Business Services Ltd. | Professional & scientific | 2007 |
| Forth Repairs & Recovery Limited | Transport & logistics | 2014 |
| Front Step Limited | Arts & recreation | 2003 |
| G.D.S Offshore Services Limited | Construction | 2013 |
| G4Q Limited | Wholesale & retail | 2023 |
| Garvennel Ltd. | Real estate | 1994 |
| Geoff Brierton Ltd | Construction | 2011 |
| Glenorchy Property Limited | Real estate | 2021 |
| Harrison Walsh Enterprises Limited | Administrative & support | 2024 |
| Hilden Homes Limited | Professional & scientific | 1965 |
| Hollywood Nails & Spa Inverkeithing Limited | Other services | 2023 |
| Hong JU Ltd | Hospitality | 2026 |
| Icosis Limited | Professional & scientific | 2002 |
| Iluty Ltd | Wholesale & retail | 2025 |
| Ironcrest Services Ltd | Wholesale & retail | 2026 |
| IWA Group Ltd | Other services | 2007 |
| Izba Limited | Real estate | 2023 |
| J Home Services Ltd. | Transport & logistics | 2025 |
| J Power Deliveries Ltd | Transport & logistics | 2026 |
| J.W.Muir (Property Investments) Limited | Real estate | 1977 |
| J.W.Muir Group Public Limited Company | Professional & scientific | 1975 |