| A Oliphant Ltd | Arts & recreation | 2022 |
| Acdn Ltd | Real estate | 2026 |
| Adrenalin Uplift Limited | Wholesale & retail | 2017 |
| Alex Media Ltd | Information & communication | 2023 |
| Andaluz Access Ltd | Mining & quarrying | 2009 |
| Andy Barlow Consultancy Ltd | Education | 2026 |
| Apex Data Intelligence Ltd | Information & communication | 2020 |
| Aston Magna Motor Company Limited | Manufacturing | 2021 |
| Audrena Ltd. | Other services | 2018 |
| Barc Coachhire Ltd | Transport & logistics | 2019 |
| BB Loamer Limited | Arts & recreation | 2023 |
| BCG Webdesign Ltd | Information & communication | 2011 |
| Bideaways Limited | Hospitality | 2019 |
| Bike Muststash Ltd | Wholesale & retail | 2023 |
| Binge Fringe Ltd | Information & communication | 2022 |
| Blagden Property Solutions Ltd | Real estate | 2013 |
| Border Commercial Repairs Limited | Wholesale & retail | 2017 |
| Borders Dynamics Limited | Information & communication | 2012 |
| Borders Local Medical Committee Limited | Health & social care | 2021 |
| Borders Takeaway Ltd | Hospitality | 2018 |
| Braw Retail Ltd | Wholesale & retail | 2024 |
| Broccabee Ltd | Professional & scientific | 2025 |
| Caldwell's Homemade Ice Cream Ltd | Hospitality | 2013 |
| CCH (Innerleithen) Ltd | Hospitality | 2014 |
| Cltmanagement Ltd | Professional & scientific | 2024 |
| Clyde Planning & Project Controls Ltd | Mining & quarrying | 2016 |
| CMX Borders Ltd | Wholesale & retail | 2022 |
| Connected Kids Limited | Information & communication | 2013 |
| Consulting Gems Ltd | Information & communication | 2025 |
| D&L Campbell Ltd | Administrative & support | 2020 |
| Decoded Electrical Ltd | Construction | 2024 |
| Deliveringcrm Limited | Information & communication | 2015 |
| Dexterity Visuals Ltd | Other services | 2020 |
| Dickson & Gould Joinery & Construction Limited | Construction | 2023 |
| Dirt School Limited | Arts & recreation | 2020 |
| Draig Energy Services Ltd | Administrative & support | 2022 |
| Droopy Dandelion Ltd | Wholesale & retail | 2018 |
| Early Experiences and Outcomes Limited | Education | 2010 |
| Edinburgh Film and Video Productions Limited | Information & communication | 1969 |
| Ellmer Ltd. | Hospitality | 2025 |
| Enduro Sports Organisation Limited | Arts & recreation | 2012 |
| Fernweh Pottery Ltd | Manufacturing | 2025 |
| Flat Cat Gallery Limited | Hospitality | 2023 |
| Form & Focus Limited | Wholesale & retail | 2026 |
| G Renwick Howford Farm | | 2000 |
| G.J. Hunter & Co Limited | Professional & scientific | 2011 |
| Garry's Convenient Store Ltd | Other services | 2018 |
| Glenevan Mill Limited | Manufacturing | 1978 |
| Glenfinn Limited | Construction | 1984 |
| Glenlude Farms | | 1997 |
| Gowaan Bike Club C.I.C. | Education | 2025 |
| Haus and Co Trading Ltd | Wholesale & retail | 2022 |
| Helen Rowland Psychotherapy Training & Supervision Ltd | Health & social care | 2024 |
| HR People First Limited | Administrative & support | 2026 |
| I-Cycles Scotland Limited | Wholesale & retail | 2022 |
| Innerleithen Community Trust | Other services | 2010 |
| Integral Learning Ltd | Education | 2016 |
| J Davidson Ltd | Construction | 2019 |
| J&S Campbell Ltd | Construction | 2020 |
| Joseph Swinton & SON Ltd | Administrative & support | 2019 |
| Kevin Lamb Consulting Limited | Professional & scientific | 2009 |
| Kirkton Range Ltd | Wholesale & retail | 2023 |
| Liminal VIS Ltd | Professional & scientific | 2026 |
| Linton Bakes Ltd | Hospitality | 2025 |
| Loulabelles Sweet Treats Ltd | Hospitality | 2018 |
| MCN Technology Limited | Information & communication | 2008 |
| Metrowest Limited | Real estate | 1997 |
| Millroo Property Ltd | Real estate | 2019 |
| Mrlmmm Ltd | Households | 2026 |
| MT Scaling Ltd | Professional & scientific | 2026 |
| Myricag Holdings Ltd | Real estate | 2025 |
| Natalie Martin Photography Limited | Professional & scientific | 2022 |
| Natural Little BEE Ltd | Wholesale & retail | 2023 |
| NO 55 Bikes Ltd | Wholesale & retail | 2025 |
| NO1 Peebles Road Ltd | Hospitality | 2018 |
| Optimus Developments Limited | Construction | 2023 |
| Peacock Property Scotland Ltd | Construction | 2022 |
| Peebles Home Helper Ltd | Administrative & support | 2023 |
| Performance Physio and Pilates Ltd | Health & social care | 2023 |
| Pro Weee Scotland Ltd | Wholesale & retail | 2025 |
| Quattro Fratelli Limited | Hospitality | 2024 |
| Rhuallan Development Ltd | Professional & scientific | 2022 |
| Ridelines (Mountain Bike Tuition) Ltd | Arts & recreation | 2011 |
| Rlmmm Ltd | Real estate | 2019 |
| Ross Hunter (Holdings) Limited | Financial services | 2016 |
| Scotlanz Limited | Construction | 2018 |
| Silverbirch Burnside Ltd | Construction | 2026 |
| Silverbirch Homes by SMH Ltd | Construction | 2019 |
| Simply Blown Away Ltd | Wholesale & retail | 2003 |
| Smith & Mcmath Group Ltd | Financial services | 2026 |
| Smith & Mcmath Limited | Construction | 2010 |
| Soonhope Ltd | Health & social care | 2026 |
| Steven RAE Accountants Ltd | Professional & scientific | 2022 |
| T Fleming Joinery Limited | Construction | 2021 |
| THE Hub Innerleithen Community Interest Company | Professional & scientific | 2019 |
| THE Hub on the High Street Ltd | Wholesale & retail | 2018 |
| THE WEE Souk Ltd. | Wholesale & retail | 2022 |
| Tiny Growers Ltd | Education | 2026 |
| Todd M Joinery Ltd | Manufacturing | 2023 |
| Tomoo Holdings Ltd | Hospitality | 2025 |