| 1/3 Aerospace Logistics Ltd | Water & waste | 2016 |
| 101 Developments Limited | Construction | 2009 |
| 112 Palm Consultancy Limited | Professional & scientific | 2025 |
| 121 Team Ltd | Professional & scientific | 2024 |
| 1538 Community Interest Company | Hospitality | 2021 |
| 1SD Midlands Limited | Real estate | 2024 |
| 1ST Choice Dental Laboratory Limited | Health & social care | 2001 |
| 1ST Transport Ltd | Transport & logistics | 2024 |
| 24 Severn Civil Engineering & Groundworks Ltd | Administrative & support | 2023 |
| 2J Prestige Car Sales Ltd | Wholesale & retail | 2026 |
| 3 D Decor Limited | Construction | 1990 |
| 30-MINUTE Crew Ltd | Arts & recreation | 2022 |
| 3JP Consulting Ltd | Professional & scientific | 2022 |
| 3Y Limited | Real estate | 2002 |
| 4D Numberplates Direct Ltd | Manufacturing | 2021 |
| 5-IR Ltd | Administrative & support | 2015 |
| 63 Gent's Hairdressing Limited | Other services | 2007 |
| @ECG Motor Holdings Limited | Wholesale & retail | 2025 |
| A & W Construction & Sons Ltd | Construction | 2011 |
| A Bedi Investments Limited | Real estate | 2014 |
| A E Houses (Midlands) Ltd | Real estate | 2024 |
| A F Surfacing & Groundworks Ltd | Construction | 2021 |
| A Force of Nature CIC | Health & social care | 2024 |
| A H Property Rentals Ltd | Real estate | 2021 |
| A J Cooper Plumbing and Heating Services Limited | Construction | 2025 |
| A K Autos (Derbyshire) Ltd | Wholesale & retail | 2015 |
| A L O Fashion & Travel Ltd | Wholesale & retail | 2020 |
| A Line Property Services Limited | Households | 2025 |
| A Muse on Food Limited | Hospitality | 2018 |
| A W Services (Derby) Limited | Administrative & support | 2010 |
| A YAP ME Dog Ltd | Other services | 2023 |
| A&B Design & Build Ltd | Construction | 2023 |
| A.I.E.W. Distribution Limited | Wholesale & retail | 2001 |
| A.J. Drury Deliveries Limited | Transport & logistics | 2003 |
| A.J. Tassi Limited | Manufacturing | 2005 |
| A.K. Engineering (Nottingham) Limited | Manufacturing | 2003 |
| A.R Meads Ltd | Wholesale & retail | 2024 |
| A.Robinson & Sons(Garages) | Wholesale & retail | 1953 |
| A.W.P. Plumbing & GAS Ltd | Construction | 2015 |
| A1 Laser Ltd | Manufacturing | 2020 |
| A7 Conversions Limited | Construction | 2015 |
| Aaartly Ltd | Wholesale & retail | 2020 |
| AAC Rentals Ltd | Real estate | 2026 |
| AAM Installations Limited | Construction | 2018 |
| AB Components Limited | Wholesale & retail | 2006 |
| AB Digital Manufacturing Ltd | Information & communication | 2026 |
| Abbey Tree Company Limited | Agriculture & fishing | 2019 |
| Abceasystream International Ltd | Information & communication | 2019 |
| Absolute Building Services (UK) Ltd | Construction | 2013 |
| Absolute Salsa Limited | Arts & recreation | 2002 |
| Aburnet (Hair Containment Solutions) Limited | Manufacturing | 2014 |
| Aburnet Consumer Messaging Solutions Limited | Manufacturing | 2023 |
| Aburnet Developments Limited | Real estate | 2014 |
| Aburnet Limited | Manufacturing | 1924 |
| AC Automotive (Ilkeston) Ltd | Wholesale & retail | 2023 |
| AC Earthing Consultancy Ltd | Professional & scientific | 2022 |
| Accent Office Interiors Ltd. | Construction | 2015 |
| Accurate Deliveries Limited | Transport & logistics | 2012 |
| Ace & Toplis Rail Ltd | Construction | 2025 |
| Ackolade Limited | Real estate | 2020 |
| ACL Contracting Ltd | Information & communication | 2025 |
| Acme Accountancy Services Limited | Professional & scientific | 2019 |
| ACT Accordingly Limited | Real estate | 2020 |
| Actualise Ltd | Professional & scientific | 2006 |
| ACW Protection Ltd | Financial services | 2024 |
| AD Joinery Services Ltd | Extraterritorial | 2019 |
| Adam Hodgkinson Plastering Limited | Construction | 1998 |
| Adams Engineering (Ilkeston) Limited | Manufacturing | 2016 |
| Addup Bookkeeping Ltd | Professional & scientific | 2016 |
| Ades Property Investment Limited | Real estate | 2020 |
| Advance Auto Recovery Ltd | Administrative & support | 2020 |
| Advanced Build (Midlands) Limited | Construction | 2019 |
| Advanced I.T (UK) Limited | Information & communication | 2012 |
| Advanced Resin Technologies (International) Ltd | Construction | 2020 |
| Advent Property Services Limited | Real estate | 2001 |
| Aero Classic Cars Ltd | Wholesale & retail | 2020 |
| Aero Road & Rail Ltd | Professional & scientific | 2025 |
| AFK Hospitality Ltd | Construction | 2026 |
| AG Consultancy Solutions Ltd | Professional & scientific | 2025 |
| AH Electrical Solutions Limited | Construction | 2016 |
| AH Timber Products Ltd | Construction | 2024 |
| AHB Direct Services Ltd | Construction | 2012 |
| Ahbs Limited | Professional & scientific | 2003 |
| Aidos Fit Ltd | Information & communication | 2026 |
| Ailsa Wood Products Ltd | Wholesale & retail | 2023 |
| Ainprivate Limited | Wholesale & retail | 2025 |
| Air-Locke Ltd | Transport & logistics | 2025 |
| Airospring Medical Ltd | Manufacturing | 2009 |
| AJG IT Consultancy Limited | Information & communication | 2020 |
| AJH Property Solutions Ltd | Real estate | 2024 |
| Ajkm Hockey Barn Limited | Arts & recreation | 2015 |
| AJL Management Consultancy Ltd | Professional & scientific | 2023 |
| AK Smalley Limited | Wholesale & retail | 2025 |
| AK Specialist Cars Ltd | Wholesale & retail | 2025 |
| Akasha Projects Ltd | Arts & recreation | 2014 |
| AKO Cuts Ltd | Other services | 2026 |
| Akqs Consulting Limited | Professional & scientific | 2024 |
| AL Investment & Consultancy Ltd | Professional & scientific | 2013 |
| Alan Chambers Electrical Ltd | Construction | 2003 |
| Alan Kent Motors Limited | Wholesale & retail | 2014 |