| 51 Travel Ltd | Administrative & support | 2023 |
| AB Premier CIC | Public administration | 2025 |
| Abacus UK Training Limited | Education | 2013 |
| ASH & SAM Investments Ltd | Real estate | 2018 |
| Beesley & Fildes Holdings Ltd | Wholesale & retail | 2018 |
| Bromley Cleaning and Facilities Management Limited | Administrative & support | 2005 |
| Carparts (NW) Limited | Wholesale & retail | 2007 |
| Carpet Design and Flooring Limited | Wholesale & retail | 1995 |
| Cranford Care Homes Limited | Health & social care | 1992 |
| Cubex (UK) Ltd | Construction | 2011 |
| Deranged Sportswear Ltd | Wholesale & retail | 2024 |
| Evobuildnw Ltd | Construction | 2023 |
| Grow (Knowsley) CIC | Professional & scientific | 2025 |
| Hardwood Connections Limited | Wholesale & retail | 2010 |
| Hardwood Sales Holdco Limited | Professional & scientific | 2026 |
| Hardwood Sales Holding Limited | Administrative & support | 2014 |
| Hardwood Sales Limited | Wholesale & retail | 2005 |
| Hardwood Sales TCO Limited | Professional & scientific | 2026 |
| Hardwood Sales Trading Limited | Wholesale & retail | 2026 |
| Hardwood Timber Sales Limited | Professional & scientific | 2001 |
| Healthwatch Knowsley C.I.C. | Health & social care | 2013 |
| Huyton & Prescot Golf Club Limited | Arts & recreation | 1948 |
| Huyton Village Limited | Hospitality | 2025 |
| Impact ARB Limited | Agriculture & fishing | 2019 |
| J & J Bricklayers Holdings Limited | Financial services | 2020 |
| JFM (Liverpool) Ltd | Wholesale & retail | 2019 |
| JMC3081 Ltd | Administrative & support | 2026 |
| JR Levins LLP | | 2024 |
| JWS Holdco Ltd | Wholesale & retail | 2018 |
| Karma in the Community C.I.C. | Arts & recreation | 2014 |
| Libraries Connected | Arts & recreation | 2011 |
| Liverpool Property Group Ltd | Real estate | 2019 |
| Locker Freight (Holdings) Limited | Financial services | 2008 |
| Mahoney Enterprise Limited | Health & social care | 2017 |
| Mainsway Limited | Transport & logistics | 1984 |
| Merseycycle C.I.C. | Wholesale & retail | 2014 |
| Merseyside Catering Fabrications Limited | Manufacturing | 2013 |
| Mind Mastery Community Limited | Education | 2024 |
| MM Garden Maintenance Services Limited | Other services | 2018 |
| Navjit J Limited | Wholesale & retail | 2010 |
| Neurodiverse Community Hub CIC | Education | 2026 |
| North West Tea Service Limited | Wholesale & retail | 2003 |
| NWT Estates Limited | Real estate | 2014 |
| Oakley Asset Management Ltd | Construction | 2013 |
| Oakley Heritage Homes Ltd | Construction | 2019 |
| One Click Supplies Limited | Wholesale & retail | 2022 |
| One Knowsley | Information & communication | 1989 |
| Platinum Shine Painters Ltd | Construction | 2026 |
| Reign Management Services Ltd | Professional & scientific | 2022 |
| Roby Garden Services Limited | Administrative & support | 2012 |
| SAM Utility Savers Limited | Other services | 2017 |
| Sanremocc Ltd | Wholesale & retail | 2025 |
| Shone Productions Limited | Arts & recreation | 2011 |
| Siobhan Lynott Limited | Professional & scientific | 2019 |
| TAG Rewards Ltd | Administrative & support | 2026 |
| THE Knowsley Chamber of Industry and Commerce | Other services | 1987 |
| Thrive Knowsley CIC | Education | 2022 |
| TMD Injectables and Aesthetics Limited | Health & social care | 2026 |
| UK Business Supplies Limited | Wholesale & retail | 2008 |
| Vantage CE Academies Trust | Education | 2012 |
| Volair Limited | Arts & recreation | 2015 |
| West Lancashire Freemasons' Charity | Health & social care | 2017 |
| Whiston Carpet Warehouse Limited | Wholesale & retail | 2000 |
| YAN Tori Limited | Hospitality | 2026 |