| Proboilers(Sussex) Limited | Construction | 2024 |
| Quaff 2 Estates Limited | Real estate | 2023 |
| Quando Quango Limited | Professional & scientific | 2011 |
| Quantum Digital Media Ltd | Professional & scientific | 2014 |
| Rhubarb Greengrocer Ltd | Wholesale & retail | 2023 |
| RI Plumbing Ltd | Construction | 2020 |
| Richmond One Limited | Real estate | 2026 |
| RST Sport Ltd | Arts & recreation | 2011 |
| SDC Wine Importers Ltd | Wholesale & retail | 2017 |
| Second Voice Ltd | Professional & scientific | 2016 |
| Sense Cycle Ltd | Wholesale & retail | 2020 |
| Shaping Tomorrow Limited | Information & communication | 2002 |
| Shep Outdoor Activities Ltd | Wholesale & retail | 2024 |
| Shovelstrode Consulting Limited | Professional & scientific | 2013 |
| Shree Krishna Foods UK Ltd | Wholesale & retail | 2025 |
| Skylark Precision Drone Solutions Ltd | Agriculture & fishing | 2025 |
| SL Electrical Sussex Limited | Construction | 2011 |
| SNB Global Consulting Ltd | Information & communication | 2024 |
| Southern Kitchens Limited | Construction | 2010 |
| Starkey Web Management Limited | Wholesale & retail | 2010 |
| Stevens Estates Limited | Real estate | 2003 |
| Stevens Lettings & Management Limited | Real estate | 2010 |
| Sussex Oak Framers Ltd | Construction | 2006 |
| Sussex Vehicle Maintenance Ltd | Wholesale & retail | 2015 |
| Svaara Ltd | Professional & scientific | 2023 |
| TEE UP Limited | Administrative & support | 2025 |
| THE Bigger Leaf Co. Limited | Professional & scientific | 2016 |
| THE Clutch Tour Limited | Professional & scientific | 2022 |
| THE Restaurant at Hickstead Limited | Professional & scientific | 2012 |
| Tidy Swan Ltd | Information & communication | 2018 |
| Tigerlily Training Ltd | Education | 2014 |
| TOM Hayward Limited | Arts & recreation | 2011 |
| Tree Tokens Limited | Agriculture & fishing | 2021 |
| Unity Challenge Limited | Arts & recreation | 2021 |
| Urbanstyle Construction Limited | Construction | 2011 |
| Valarian Limited | Wholesale & retail | 2020 |
| VIA Polaris Ltd | Information & communication | 2025 |
| West Down Legal Ltd | Professional & scientific | 2011 |