| Quest Investments Limited | Financial services | 1992 |
| R & a Sports Medicine Limited | Health & social care | 2015 |
| R G Ward Building Ltd. | Construction | 2019 |
| Random Mandem Limited | Information & communication | 2015 |
| Redstar Online Limited | Wholesale & retail | 2016 |
| Redwood Landscapes (Hathersage) Limited | Administrative & support | 2006 |
| Redwood Roofing Limited | Construction | 2026 |
| REG Clark Properties Limited | Real estate | 2012 |
| Retail IT Solutions Ltd | Information & communication | 2016 |
| Richard Gilbourne Engineering Limited | Manufacturing | 2003 |
| Ridgeway Business Systems Limited | Information & communication | 1981 |
| Risheash Limited | Professional & scientific | 2020 |
| Riverside Garage (Bamford) Limited | Wholesale & retail | 2003 |
| Riverside Holiday Park Limited | Hospitality | 2015 |
| RJA Contracting Limited | Construction | 2015 |
| Roll OUT the Barrel Limited | Hospitality | 2024 |
| Rose Cottage Castleton Ltd | Hospitality | 2024 |
| Rose Cottage Hospitality (Castleton) Limited | Hospitality | 2026 |
| Rose Pavilion Partners Limited | Financial services | 2026 |
| Round Shot Limited | Real estate | 2020 |
| Salts Fish Bar Limited | Hospitality | 2017 |
| Saltstreet Productions Limited | Information & communication | 2014 |
| Sammans Investment Ltd | Real estate | 2022 |
| Sandy's Holiday Cottages Ltd | Hospitality | 2019 |
| Sanrish Limited | Real estate | 2020 |
| Satori Properties Ltd | Real estate | 2017 |
| Seamoor Creative Limited | Information & communication | 2017 |
| Seaton Structural Ltd | Professional & scientific | 2011 |
| Seven Acres Property Limited | Real estate | 2021 |
| Seven Gingers Ltd | Hospitality | 2019 |
| Seven Hills Chimney Sweeps Limited | Construction | 2024 |
| Shape.Build Limited | Information & communication | 2020 |
| Sheaf Bank Business Park Limited | Real estate | 2002 |
| Sheaf Investment Company Limited | Administrative & support | 1957 |
| Sheaf Motors Limited | Real estate | 1982 |
| Sheaf Motors of Coal Aston Limited | Real estate | 1939 |
| SHED-10 Research and Event Services Ltd | Information & communication | 2008 |
| Shivering Mountain Distillery Limited | Manufacturing | 2019 |
| Shivering Mountain Limited | Manufacturing | 2019 |
| Sickleholme Golf Club,Limited(The) | Arts & recreation | 1920 |
| Sikey Ltd | Construction | 2026 |
| Silverweb Spirit Limited | Information & communication | 2024 |
| Simon Housley PGA Ltd | Wholesale & retail | 2019 |
| Simple Industrial Machinery Safety Ltd | Professional & scientific | 2021 |
| Simple Things Project(Peak District) CIC | Administrative & support | 2026 |
| Simpson Refractories Limited | Construction | 2002 |
| Sizzle Group Limited | Administrative & support | 2016 |
| Sizzle Live Events Limited | Administrative & support | 2016 |
| Sizzle PR Limited | Administrative & support | 2016 |
| SJW Architects Ltd | Professional & scientific | 2015 |
| Skyline Ecology Ltd | Extraterritorial | 2013 |
| Skyline Ventures Ltd | Professional & scientific | 2013 |
| Sladen Consulting Limited | Professional & scientific | 2009 |
| SM Maintenance Limited | Manufacturing | 2011 |
| Smartovations EOT Ltd | Financial services | 2025 |
| Sowerby Landscapes Limited | Administrative & support | 2023 |
| Speedwell Cavern Limited | Wholesale & retail | 1973 |
| Sport Event Solutions Limited | Professional & scientific | 2005 |
| Spring House Farm Ltd | Hospitality | 2026 |
| Spring Planning Ltd | Professional & scientific | 2017 |
| Stanage Estates Ltd | Real estate | 2024 |
| Stanage House Farm Ltd | Agriculture & fishing | 2019 |
| Stanage Limited | Professional & scientific | 2015 |
| Steel City Coaching Ltd | Arts & recreation | 2026 |
| Stoke Stoneworks Limited | Manufacturing | 2020 |
| Stoke Waste Collective Limited | Water & waste | 2018 |
| Stonetrough Data Mining LLP | | 2009 |
| Stoneymoon Limited | Hospitality | 2015 |
| Strategik Creative Limited | Information & communication | 2013 |
| Studyhub Limited | Information & communication | 2003 |
| Sunnybank Nursery Ltd | Health & social care | 2017 |
| Sustainable Edale CIC | Professional & scientific | 2024 |
| Swallowholme Camping & Caravan Park Ltd | Construction | 2009 |
| Swiss Hotel Ltd | Hospitality | 2021 |
| Switch & Go Limited | Manufacturing | 2024 |
| Sykes Property Group Ltd | Construction | 2024 |
| T M S Europe Limited | Manufacturing | 1991 |
| T Price Properties Limited | Construction | 2018 |
| T.H.Knightall Limited | Manufacturing | 1982 |
| Talbot Court (Eyam) Management Company Limited | Households | 2000 |
| Taverna Inns Limited | Extraterritorial | 1990 |
| Taylor's Place Ltd | Hospitality | 2023 |
| TCA Building Consultancy Ltd | Professional & scientific | 2020 |
| TEW Group Limited | Real estate | 1997 |
| THE Castleton Historical Society | Arts & recreation | 1999 |
| THE Cattle Shed Limited | Hospitality | 2015 |
| THE Ccat Project Ltd | Information & communication | 2025 |
| THE Cheshire Cheese Limited | Hospitality | 2010 |
| THE Church Fund Ltd | Real estate | 2016 |
| THE Dbad Project Ltd | Professional & scientific | 2023 |
| THE Derbyshire Gift Centre Limited | Wholesale & retail | 1998 |
| THE Edge Farm Shop Ltd | Wholesale & retail | 2020 |
| THE Girls' Brigade England and Wales | Other services | 1925 |
| THE Happy Tree Company (Hope Valley) Limited | Administrative & support | 2005 |
| THE Herb Table Limited | Hospitality | 2001 |
| THE Hope Valley Centre Ltd | Wholesale & retail | 2021 |
| THE Housley Bequest Limited | Professional & scientific | 2001 |
| THE Joinery Workshop (Derbyshire) Limited | Manufacturing | 2006 |
| THE Lodge House NHP Limited | Real estate | 2007 |
| THE National Response Academy Limited | Professional & scientific | 2016 |