| Adams Osteopathy Ltd | Education | 2025 |
| ALI Gordon Ltd | Administrative & support | 2017 |
| Apron Coffee & Snaxx Shack Limited | Hospitality | 2022 |
| Atcloud Limited | Professional & scientific | 2021 |
| Body Friendly Limited | Extraterritorial | 2015 |
| Bottle Bazaar Ltd | Hospitality | 2013 |
| Callan Retail Consulting Limited | Administrative & support | 2023 |
| Clare House Property Ltd | Real estate | 2024 |
| Esselle Strategies Ltd | Professional & scientific | 2012 |
| Golden Bear Futures Ltd | Financial services | 2025 |
| Hera Photography Ltd | Professional & scientific | 2019 |
| HRS One Limited | Real estate | 2023 |
| Hunza Consultancy Services Ltd | Information & communication | 2023 |
| L'Hôte Ltd | Hospitality | 2018 |
| LEM Online Ltd | Administrative & support | 2016 |
| Lionwing Limited | Information & communication | 2002 |
| Love Lane Books Ltd | Information & communication | 2011 |
| MI Wealth Consultancy Limited | Professional & scientific | 2021 |
| Millstock Trading Limited | Wholesale & retail | 1989 |
| North Herts Surface Repair Limited | Professional & scientific | 2019 |
| Offley Place Limited | Hospitality | 2003 |
| Orca Academy Ltd | Education | 2026 |
| OZ & Osaman's Ltd | Real estate | 2023 |
| Propac Consulting Ltd | Administrative & support | 2024 |
| SKC Films Limited | Administrative & support | 2015 |
| SOH Group Limited | Other services | 2017 |
| SOH Pure Limited | Other services | 2021 |
| Templars Property Management Ltd | Hospitality | 2020 |
| THE Mushroom Emporium Limited | Agriculture & fishing | 2021 |
| THE Stevenage Counselling Clinic Ltd | Health & social care | 2022 |
| Webster & Hamilton Ltd | Real estate | 2020 |