| DS Recovery & Transport Ltd | Transport & logistics | 2024 |
| DSB Estates Limited | Real estate | 2021 |
| DSB Property Development Limited | Construction | 2014 |
| DSE Investments Ltd | Financial services | 2024 |
| Dtea Limited | Professional & scientific | 2020 |
| DTL Group Ltd | Real estate | 2018 |
| DTP Surfacing Limited | Construction | 2020 |
| Dufex Craft Products Limited | Extraterritorial | 2010 |
| Dunmore Developments Limited | Construction | 2000 |
| Dunstable Meat Company Limited | Real estate | 1959 |
| Dura Performance Limited | Information & communication | 2025 |
| Duryard Developments Limited | Construction | 2022 |
| Dwmt Prestige Ltd | Administrative & support | 2026 |
| DXN Consulting Limited | Professional & scientific | 2023 |
| Dynamic Edge Software Ltd | Information & communication | 2010 |
| Dynamic Security Management Limited | Administrative & support | 2019 |
| Dynasty Cricket Ltd | Manufacturing | 2025 |
| DZ VFX Limited | Information & communication | 2025 |
| E C Southsea Limited | Hospitality | 2023 |
| E Hayes Holdings Ltd | Financial services | 2022 |
| E Micutsu Ltd | Transport & logistics | 2026 |
| E&A Wealth Ltd | Professional & scientific | 2020 |
| E&W Services Ltd | Construction | 2020 |
| E-Lux Electrical Limited | Construction | 2022 |
| E.P.A. Kimpton Limited | Real estate | 1977 |
| E.P.A. Products Limited | Manufacturing | 1995 |
| E.T.Cherry & Sons Limited | Wholesale & retail | 1959 |
| E14 PH Ltd | Hospitality | 2026 |
| E360 Recruiting Ltd | Extraterritorial | 2022 |
| EA Spring Limited | Administrative & support | 2015 |
| Eagle EYE Digital and Production Ltd | Information & communication | 2025 |
| Early Days Pre-School | Education | 2012 |
| Early Years Excellence Academy Ltd | Education | 2024 |
| Earthworks Studios Limited | Arts & recreation | 2016 |
| Easitech Limited | Information & communication | 2016 |
| East Africa Careers Guidance Practitioners Association | Education | 2008 |
| East Anglia CW Limited | Health & social care | 2023 |
| East Anglia Event Hire Ltd | Administrative & support | 2022 |
| East Asia Whisky Company Ltd | Wholesale & retail | 2021 |
| East Wind Healthcare Ltd | Health & social care | 2023 |
| Easy Talent Ltd | Administrative & support | 2022 |
| Easy Technology Group Ltd | Information & communication | 2022 |
| EAT Catering Bedfordshire Ltd | Hospitality | 2023 |
| Eaves Electrical Ltd | Construction | 2019 |
| EB Medical Ltd | Health & social care | 2024 |
| Eben Home Limited | Construction | 2018 |
| Ebgm Consulting Ltd | Professional & scientific | 2023 |
| Ebryl Limited | Administrative & support | 2003 |
| EBS Aviation Ltd | Professional & scientific | 2026 |
| EC Coffee North Holdings Limited | Financial services | 2024 |
| EC Company 1 Limited | Hospitality | 2024 |
| EC Company 3 Limited | Hospitality | 2024 |
| EC Company 4 Limited | Hospitality | 2024 |
| EC Company 5 Limited | Hospitality | 2024 |
| EC Leasehold Estate Limited | Real estate | 2021 |
| EC Yorkshire Ltd | Wholesale & retail | 2024 |
| Ecocrate Packaging Ltd | Wholesale & retail | 2025 |
| Ecoelek Limited | Construction | 2014 |
| Ecomfort Heating Limited | Construction | 2019 |
| Ecoterms Limited | Real estate | 1996 |
| Ecovril Limited | Professional & scientific | 2011 |
| Ecrin Ltd | Hospitality | 2026 |
| ED Kaizen Properties Ltd | Households | 2026 |
| ED Purple Limited | Real estate | 2026 |
| Edcreations Ltd | Wholesale & retail | 2004 |
| Eden View Management Company Limited | Households | 2013 |
| Edge Digital Services Limited | Information & communication | 2014 |
| Edge Line Products Limited | Construction | 2018 |
| EDUC8 (Luton) Ltd | Education | 2016 |
| Educate Private Ltd | Education | 2012 |
| Eerd Technologies Ltd | Information & communication | 2024 |
| Efficacious Limited | Professional & scientific | 2010 |
| Effra Properties Limited | Real estate | 2024 |
| Efollo Limited | Transport & logistics | 2009 |
| EGG Head Productions Ltd | Information & communication | 2026 |
| EH Fitness Ltd | Arts & recreation | 2020 |
| EH Mouldings Limited | Extraterritorial | 2024 |
| Eilis Ltd | Real estate | 2021 |
| EJC Trading Ltd | Health & social care | 2024 |
| EJS Turbine Services Ltd | Professional & scientific | 2020 |
| EKH Medical Communications Limited | Professional & scientific | 2017 |
| Ekiro Limited | Administrative & support | 2023 |
| EKM Services Ltd | Administrative & support | 2004 |
| Ekoo Barbers Ltd | Other services | 2025 |
| Elation Nation Limited | Arts & recreation | 2026 |
| ELDERCARE360 Ltd | Administrative & support | 2026 |
| Elderflower Investments Ltd | Real estate | 2019 |
| Electrical Tradesmen Limited | Construction | 2010 |
| Electricity Compliance Services Ltd | Construction | 2022 |
| ELEV8 Software Limited | Information & communication | 2026 |
| Elevici Ltd | Professional & scientific | 2020 |
| Eley Engineering Services Limited | Wholesale & retail | 2015 |
| Elfin Investments Limited | Real estate | 2021 |
| Elhan Heating Ltd | Construction | 2016 |
| Elion Consulting Ltd | Professional & scientific | 2024 |
| Elisexell Limited | Transport & logistics | 2009 |
| Elite Document Solutions Limited | Information & communication | 1999 |
| Elite Estates Investments Limited | Real estate | 2026 |
| Elite Medics Ltd | Health & social care | 2008 |
| Elite Profiles Limited | Wholesale & retail | 2007 |