| Allied Mechanical Services Limited | Construction | 1985 |
| Andrew Winterbottom Imaging and Intervention Limited | Health & social care | 2026 |
| Art of Libs Ltd | Arts & recreation | 2016 |
| Assurewills Limited | Professional & scientific | 2009 |
| Bazari Wahab Holdings Limited | Financial services | 2021 |
| Blakes Estimating & Surveying Services Limited | Professional & scientific | 2003 |
| BOB Dixon (Cambridge) Limited | Arts & recreation | 1992 |
| Brian Freeborn Associates Limited | Professional & scientific | 1996 |
| Cambridge City Properties Limited | Real estate | 2026 |
| Cambridge Fine Art Limited | Wholesale & retail | 2021 |
| Cambridge Gastrocare Limited | Health & social care | 2021 |
| Chapel Hill Capital Limited | Administrative & support | 2021 |
| Christian Barnett Ltd | Professional & scientific | 2009 |
| Cinaps Ltd | Health & social care | 2009 |
| Clinical Teledermatology Ltd | Health & social care | 2024 |
| Cumulocity (UK) Ltd | Information & communication | 2023 |
| David Honeyman Associates Limited | Other services | 2003 |
| Ebap Limited | Information & communication | 2006 |
| G P Motors Limited | Wholesale & retail | 1993 |
| Hammond’S Mortgage Services Limited | Financial services | 2007 |
| Houghton Homes (Cambridge) Limited | Construction | 1952 |
| Iqbro Limited | Hospitality | 2013 |
| J & JW Limited | Professional & scientific | 2013 |
| Lehmann Maupin UK Limited | Wholesale & retail | 2020 |
| Linkx Global Ltd | Wholesale & retail | 2025 |
| MAS Environmental Limited | Professional & scientific | 2011 |
| Mias Pharma UK Limited | Professional & scientific | 2023 |
| MMP Tax Limited | Professional & scientific | 2012 |
| Montagu Arts Limited | Administrative & support | 2013 |
| Musculoskeletal Imaging & Education Limited | Health & social care | 2017 |
| Netcracker Technology Emea Limited | Information & communication | 1989 |
| Nichino Europe Co., Ltd. | Manufacturing | 2007 |
| Picture Estates Limited | Construction | 2021 |
| Pleasance, Hookham & NIX Limited | Professional & scientific | 2006 |
| Pliteq (UK) Limited | Administrative & support | 2011 |
| Poset Limited | Construction | 2017 |
| Rheostrata Limited | Administrative & support | 2025 |
| Rubrahealth Limited | Information & communication | 2024 |
| S Mercer Properties Limited | Professional & scientific | 2012 |
| Sajjad ALI Investments LLP | | 2019 |
| Sensorhut Ltd | Professional & scientific | 2013 |
| SJH Medical Consulting Limited | Health & social care | 2022 |
| Spincar UK Limited | Wholesale & retail | 2019 |
| SPR Doctor Limited | Health & social care | 2017 |
| Suffolk Premier Homes Limited | Real estate | 2006 |
| THE Secret Sanctum Limited | Wholesale & retail | 2020 |
| THE William Templeton Foundation for Young People's Mental Health | Health & social care | 2019 |
| This Land Limited | Construction | 2016 |
| Under OUR Umbrella Limited | Information & communication | 2010 |
| Valou Limited | Education | 2025 |
| Veeco Instruments Limited | Manufacturing | 1989 |
| Visiativ Solutions UK Limited | Information & communication | 2002 |
| Warneswest Ltd | Professional & scientific | 2014 |
| Witnet Ltd | Real estate | 2017 |
| Zarkos Construction Ltd | Construction | 2023 |
| Zarkos-Smith Ltd | Professional & scientific | 2018 |