| Emissions Analytics Limited | Professional & scientific | 2011 |
| Emma Jane Walker Limited | Professional & scientific | 2019 |
| Emma York Clinic Ltd | Health & social care | 2016 |
| Empire 9 Building Maintenance Ltd | Construction | 2023 |
| Empire Care and Support Ltd | Health & social care | 2018 |
| Empire Effects Limited | Information & communication | 2012 |
| Empire Trading Limited | Wholesale & retail | 2006 |
| Empire Vista Holdings Ltd | Financial services | 2024 |
| Emporium Enteprise Limited | Wholesale & retail | 2022 |
| Empower Supported Housing CIC | Health & social care | 2024 |
| Empowered Health Ltd | Health & social care | 2019 |
| Emrys Development Ltd | Education | 2026 |
| Emrys Group Ltd | Administrative & support | 2022 |
| EMS Construction and Services Ltd | Construction | 2023 |
| Emtec Products Limited | Construction | 1996 |
| Emvolve Ltd | Construction | 2020 |
| EN EE Accounting Ltd | Professional & scientific | 2013 |
| Enamelin Ltd | Professional & scientific | 2021 |
| Enchanted Elegance Ltd | Other services | 2026 |
| Encompass Roofing Services Limited | Construction | 1995 |
| END Result Limited | Administrative & support | 2003 |
| Enecoma Consultancy Limited | Information & communication | 2018 |
| Energizer Auto UK Limited | Manufacturing | 1989 |
| Energizer Auto UK Parent Limited | Professional & scientific | 2010 |
| Energizer Brands UK Limited | Wholesale & retail | 2016 |
| Energizer Group Limited | Wholesale & retail | 2000 |
| Energizer Trading Limited | Wholesale & retail | 1986 |
| Energizer UK Limited | Wholesale & retail | 2015 |
| Energy Support Team Ltd | Professional & scientific | 2003 |
| Enerzon Ltd. | Wholesale & retail | 2010 |
| Enforma Limited | Professional & scientific | 2016 |
| Engage Digital Limited | Information & communication | 2012 |
| Engel Property Holdings Ltd | Real estate | 2025 |
| Engineering Skills Competitions CIC | Education | 2020 |
| Engrave-It UK Limited | Manufacturing | 2000 |
| Engtronic Limited | Manufacturing | 2015 |
| Enigma Integration Limited | Wholesale & retail | 2018 |
| Enlight Data Ltd | Wholesale & retail | 2024 |
| Enlightened CTI Solutions Ltd. | Information & communication | 2017 |
| ENM Car Sales Ltd | Wholesale & retail | 2025 |
| Ennoidgy Limited | Professional & scientific | 2019 |
| Enrich Creative Ltd | Professional & scientific | 2013 |
| Entalis Ltd | Information & communication | 2018 |
| Enteetainment Limited | Arts & recreation | 2008 |
| Entegraty Rainbird Business Solutions Ltd | Information & communication | 2020 |
| Entek Ltd | Manufacturing | 2018 |
| Entigy Associates Limited | Professional & scientific | 2013 |
| Entigy Financial Services Limited | Financial services | 2014 |
| Entigy Ltd | Information & communication | 1991 |
| Entigy Software Limited | Information & communication | 2012 |
| Envirogenix Ltd | Manufacturing | 2023 |
| Environmental Cleaning Contractors Ltd | Administrative & support | 2025 |
| Envirotec Limited | Manufacturing | 1980 |
| Envision Digital Ltd | Professional & scientific | 2019 |
| Envospace Limited | Manufacturing | 2020 |
| Enzo Srilankan Street Food Ltd | Hospitality | 2019 |
| Eocito Employee Ownership Trustee Limited | Professional & scientific | 2021 |
| Eocito Limited | Information & communication | 2019 |
| EP Former Trading Co Limited | Administrative & support | 1957 |
| EP Newco 1 Limited | Manufacturing | 2015 |
| EP Newco 2 Limited | Real estate | 2015 |
| EP Newco 3 Limited | Real estate | 2016 |
| Epcot Career Solutions Limited | Education | 2010 |
| Epds Home Improvement Ltd | Construction | 2026 |
| Epic Health Limited | | 2011 |
| Epic Security Ltd | Information & communication | 2022 |
| Epirrion Ltd | Other services | 2017 |
| Epos Worldwide Ltd | Professional & scientific | 2018 |
| EQ Evolutio Limited | Professional & scientific | 2007 |
| Equilibrium Body, Mind & Spirit Ltd | Health & social care | 2025 |
| Equinibria Ltd | Education | 2024 |
| Equinox Automotive Ltd | Wholesale & retail | 2023 |
| Equinum Limited | Agriculture & fishing | 2018 |
| Equiopportunity Ltd | Administrative & support | 2022 |
| Equishare Associates Ltd | Extraterritorial | 2024 |
| Equishare Holdings Ltd | Financial services | 2024 |
| Equity in Tech Group Limited | Information & communication | 2024 |
| Erays Global Ltd | Wholesale & retail | 2025 |
| Ergo 88 Limited | Financial services | 2025 |
| Ernaville and Glendower Residents Ltd | Households | 2015 |
| Erpbase Ltd | Information & communication | 2015 |
| Erpbot Ltd | Information & communication | 2018 |
| ERR Games Limited | Manufacturing | 2017 |
| Errington Knight Refurbishment Ltd | Construction | 2018 |
| ES1MARKETING Ltd | Professional & scientific | 2025 |
| Esandjay Limited | Real estate | 2016 |
| Esant Limited | Real estate | 2016 |
| Escapada Ltd | Administrative & support | 2025 |
| Eskdale Management Company Limited | Households | 2020 |
| ESL Electrical Solutions Ltd | Construction | 2014 |
| Esmeridian Holdings Ltd | Construction | 2026 |
| Esmil Process Systems Limited | Construction | 1988 |
| Esmn Capital Limited | Professional & scientific | 2024 |
| Esplorio Limited | Information & communication | 2012 |
| Espoir GK Limited | Arts & recreation | 2021 |
| Espresso Vibes Limited | Manufacturing | 2021 |
| Espresso Vibes Mobile Limited | Hospitality | 2024 |
| Essendtial Ltd | Education | 2025 |
| Essensi Ltd | Wholesale & retail | 2024 |
| Essentials Cleaning Services Ltd | Administrative & support | 2009 |