| Scaddings Ltd | Wholesale & retail | 2024 |
| Sciwrite Ltd | Health & social care | 2016 |
| Securi-Flex Ltd. | Wholesale & retail | 1999 |
| Sehc Consulting Limited | Administrative & support | 2022 |
| SELL4PROFIT Ltd | Wholesale & retail | 2016 |
| Sellwood Media Limited | Professional & scientific | 2013 |
| Serna Construction Ltd | Construction | 2023 |
| Serviced Office Company Ltd | Real estate | 2007 |
| Servicing Sussex Limited | Wholesale & retail | 2021 |
| Sewa Coti Ltd. | Mining & quarrying | 2014 |
| SFB Equestrian Limited | Other services | 2013 |
| Sfwr Ltd | Construction | 2020 |
| SFX Cables Ltd | Extraterritorial | 2019 |
| Shanahan Sport Horses Ltd | Agriculture & fishing | 2022 |
| Shannon Consulting Ltd | Professional & scientific | 2023 |
| Shannon Park Estates Limited | Real estate | 2025 |
| Sheridan & Fromant Ltd | Wholesale & retail | 2024 |
| Sheridan Installations Limited | Information & communication | 2002 |
| Shiprods Farm Limited | Agriculture & fishing | 2005 |
| Shirfield Consulting Limited | Other services | 2025 |
| Shoppee Homes Limited | Real estate | 1997 |
| Shoreclean Limited | Administrative & support | 2011 |
| Shoreham Bike Park CIC | Arts & recreation | 2021 |
| Shree Nilkanth Ltd | Real estate | 2025 |
| Silver Forest Partnership Ltd | Professional & scientific | 2014 |
| Simon Benjamin Limited | Manufacturing | 2016 |
| Simple Preacher Ltd | Other services | 2024 |
| Sisterly Love Limited | Professional & scientific | 2011 |
| Site Defender Limited | Administrative & support | 2017 |
| Skrinkle Reservoir Geology Ltd | Professional & scientific | 2012 |
| SLB Digital Limited | Information & communication | 2013 |
| SLC Imagery Ltd | Professional & scientific | 2023 |
| Smart Tech Tools Limited | Wholesale & retail | 2011 |
| Smartstart Home Automation Ltd | Construction | 2023 |
| Smican Ltd | Wholesale & retail | 2022 |
| Smith & Torok Ltd | Manufacturing | 2013 |
| Solidarity Merchandise Ltd | Wholesale & retail | 2020 |
| Solo the Training Club Ltd | Arts & recreation | 2021 |
| Source Specialist Property Finance Ltd | Financial services | 2019 |
| South Coast Commercials Sussex Ltd | Wholesale & retail | 2012 |
| South Coast Construction and Services Ltd | Construction | 2025 |
| South Coast Conveyancing Ltd | Extraterritorial | 2025 |
| South Coast Solid Surfacing Ltd | Manufacturing | 2024 |
| South Down Treasures Ltd | Wholesale & retail | 2024 |
| South Downs Flint Ltd | Manufacturing | 2013 |
| South Downs Spirits Company Limited | Manufacturing | 2021 |
| South View Residents Association Limited | Extraterritorial | 2003 |
| Southbank Equestrian Centre LLP | | 2018 |
| Southdown Engineers Limited | Transport & logistics | 1997 |
| Southdown Farming Ltd | Agriculture & fishing | 2026 |
| Southdown Properties Ltd | Extraterritorial | 2021 |
| Southdowns Flyfishing CIC | Agriculture & fishing | 2025 |
| Southdowns Gutter Cleaning Ltd | Administrative & support | 2026 |
| Southern Beeches Limited | Agriculture & fishing | 2001 |
| Southern Scientific Limited | Manufacturing | 1984 |
| Sparks Cars Limited | Wholesale & retail | 1997 |
| Spearing Associates, LLP | | 2012 |
| SSB Scaffolding South East Ltd | Construction | 2018 |
| Stedsay Controls Limited | Professional & scientific | 2013 |
| Sterling Technical Hvac Services Ltd | Energy | 2019 |
| Steve Bray Ltd | Wholesale & retail | 2017 |
| Stonepit Nurseries Limited | Wholesale & retail | 1972 |
| Store 2 ME Ltd | Wholesale & retail | 2025 |
| Strachan Plant Services Ltd | Manufacturing | 2026 |
| Strikr Boxing Ltd | Wholesale & retail | 2025 |
| Succeed Cars Limited | Hospitality | 2024 |
| Suffolk Business Flowers Limited | Other services | 2015 |
| Surviving Christmas Limited | Information & communication | 2016 |
| Sussex CNC Services Ltd | Extraterritorial | 2022 |
| Sussex Emdr Services Ltd | Education | 2025 |
| Sussex Fibreglass & Plastics Ltd | Manufacturing | 2023 |
| Sussex Flowers Limited | | 1979 |
| Sussex Heritage Damp Restoration (Shdr) Limited | Construction | 2024 |
| Sussex Landscaping Design Limited | Administrative & support | 2019 |
| Sussex Oak Structures Limited | Construction | 2002 |
| Sussex Orthopaedics Ltd | Health & social care | 2024 |
| Sussex Prairies Ltd | Agriculture & fishing | 2005 |
| Sussex Roof Windows Ltd | Construction | 2021 |
| T & W Scaffolding Ltd | Construction | 2016 |
| Taberah Ltd | Information & communication | 2022 |
| Talk for Change Ltd | Health & social care | 2024 |
| Taylored Carpentry & Building Ltd | Construction | 2024 |
| Team Van Oord Limited | Construction | 2012 |
| TECH70 Ltd | Information & communication | 2025 |
| Teck Revive Ltd | Professional & scientific | 2025 |
| Tekate Limited | Information & communication | 2024 |
| Tenant Management (5) Limited | Hospitality | 2004 |
| Tendai International CIC | Education | 2024 |
| THE Budding Camellia Limited | Wholesale & retail | 2002 |
| THE Business Support Academy Limited | Professional & scientific | 2006 |
| THE Chalet Transport Cafe Limited | Hospitality | 2011 |
| THE Chilli Trail Limited | Manufacturing | 2025 |
| THE Devereux Partnership LLP | | 2005 |
| THE Fitness Barn Ltd | Arts & recreation | 2024 |
| THE Green Management Company Limited | Real estate | 1996 |
| THE Grommet Company.Com Ltd | Extraterritorial | 2016 |
| THE Haxted Flyers Limited | Arts & recreation | 2015 |
| THE Henfield Fencing Company Ltd | Construction | 2015 |
| THE Lady Gardener Team Ltd | Administrative & support | 2022 |
| THE Last Bite Ltd | Hospitality | 2025 |