| Emerald International Ltd | Wholesale & retail | 2024 |
| Emesord Limited | Information & communication | 2025 |
| Emesse Ltd | Professional & scientific | 2020 |
| Emico Holdings Limited | Financial services | 2015 |
| Emico Limited | Construction | 1997 |
| Emico Property Services Limited | Extraterritorial | 2015 |
| Emily's Moments Photography Ltd | Professional & scientific | 2022 |
| Emma West Consulting Limited | Professional & scientific | 2014 |
| Emme & Co Holdings Ltd | Administrative & support | 2026 |
| Empire Electrical Innovations Ltd | Construction | 2024 |
| Empire Scaffolding Contractors Limited | Construction | 2025 |
| Empowertalk Ltd | Information & communication | 2023 |
| Empty Box Recruitment Limited | Administrative & support | 2004 |
| Emrys Haulage Ltd | Transport & logistics | 2017 |
| EMT Corporation Limited | Administrative & support | 2019 |
| EMT Inc (UK) Limited | Administrative & support | 2019 |
| Emwhy Co Ltd. | Wholesale & retail | 2018 |
| Enchantebelle Ltd | Other services | 2020 |
| Enchanted Goods Ltd | Wholesale & retail | 2024 |
| Endeavour Resourcing Solutions Limited | Administrative & support | 2023 |
| Energy Support Servicing Ltd | Professional & scientific | 2020 |
| Enertek Group Limited | Administrative & support | 2013 |
| Engage 2 Achieve Ltd | Information & communication | 2012 |
| Engage Arts CIC | Manufacturing | 2019 |
| Engine Workshop Ltd | Wholesale & retail | 2017 |
| Engineering HRS (UK) Ltd | Construction | 2019 |
| Engineering Recruitment Specialists Ltd | Administrative & support | 2020 |
| Engineering Systems (EEC) Limited | Real estate | 1984 |
| Engineering-Me Ltd | Professional & scientific | 2024 |
| English Sangha Trust Limited(The) | Other services | 1956 |
| Enhakkore Partners Limited | Real estate | 2023 |
| Enhance by Design Limited | Construction | 2004 |
| Enigma Plastering Group Ltd | Construction | 2025 |
| Enluxe Electrical Ltd | Construction | 2021 |
| Enluxe Properties Ltd | Real estate | 2024 |
| Ensentia Ltd | Information & communication | 2009 |
| Ensun Ltd | Professional & scientific | 2016 |
| Entech Technical Solutions Limited | Administrative & support | 1998 |
| Enterprise House (Hemel) Limited | Real estate | 2016 |
| Enterprise Property Bureau Ltd | Real estate | 2024 |
| Entertec Limited | Arts & recreation | 2005 |
| Entoplast Ltd | Professional & scientific | 2021 |
| ENV Cool Ltd | Energy | 2022 |
| Enventus Limited | Professional & scientific | 2014 |
| Enviera Ltd | Administrative & support | 2024 |
| Enviro Design Associates Ltd | Professional & scientific | 2012 |
| Environmental Medicine Foundation | Other services | 2003 |
| Envisage Base Ltd | Information & communication | 2025 |
| Envision Property Ventures Ltd | Financial services | 2024 |
| Envoplan Limited | Construction | 2020 |
| Enwongo&Id Consultancy Limited | Information & communication | 2026 |
| EP Sound Ltd | Manufacturing | 2026 |
| Epdm Consulting Ltd | Professional & scientific | 2024 |
| Epedals Ltd | Construction | 2023 |
| Epiphyte Consulting Limited | Information & communication | 2007 |
| Epiroc UK & Ireland Limited | Professional & scientific | 2017 |
| Episode Oberoi Leamington Hotels Limited | Hospitality | 2018 |
| Eposability Limited | Information & communication | 2008 |
| Equal Accountancy Ltd | Professional & scientific | 2020 |
| Equipp Ltd | Wholesale & retail | 2017 |
| Equipped Valeting Ltd | Administrative & support | 2023 |
| ER Contracts Ltd | Construction | 2023 |
| Eralux Carpentry Ltd | Construction | 2023 |
| Erga Productions Limited | Information & communication | 2008 |
| Erica Dorn Limited | Professional & scientific | 2019 |
| Eridian Digital Ltd | Professional & scientific | 2026 |
| Ermg Limited | Financial services | 2016 |
| ERP360PRO Ltd | Wholesale & retail | 2020 |
| Errs Limited | Health & social care | 2014 |
| Ersham Park (Hailsham) Management Company Limited | Households | 2022 |
| Erste Capital Ltd | Professional & scientific | 2020 |
| Esca Design Ltd | Professional & scientific | 2025 |
| Escher Property Ltd | Professional & scientific | 2014 |
| Esdac Ltd | Information & communication | 2012 |
| Esmaeili & Co Limited | Other services | 2013 |
| Esmart Global Ltd | Information & communication | 2017 |
| Esmyda Ltd | Hospitality | 2024 |
| Esom Developments Ltd | Construction | 2026 |
| Espc Holdings London Ltd | Real estate | 2019 |
| Espresso Greek Bakery Limited | Wholesale & retail | 2024 |
| Esra Solutions Limited | Construction | 2017 |
| Essedum Ltd | Manufacturing | 2025 |
| Essendon Park Management Company Limited | Households | 2007 |
| Essential Contract Services Limited | Real estate | 2014 |
| Essential Performance Consulting Ltd | Professional & scientific | 2009 |
| Essential Vivendi Construction Limited | Construction | 2006 |
| Essential Vivendi Flixton Limited | Real estate | 2018 |
| Essential Vivendi Limited | Real estate | 2006 |
| Essential Vivendi Management Limited | Extraterritorial | 2006 |
| Estimate Software Limited | Information & communication | 1998 |
| Esto Global Ltd | Manufacturing | 2019 |
| Etaerio Ltd | Professional & scientific | 2018 |
| Etailup Ltd | Wholesale & retail | 2018 |
| Ethan Evans Ltd | Administrative & support | 2026 |
| Ethica Partnerships Ltd | Administrative & support | 2024 |
| Ethnic Herts Ltd. | Professional & scientific | 2017 |
| ETM Logistics Ltd | Transport & logistics | 2019 |
| Eton Gardens Management Company Ltd | Households | 2005 |
| Etop Global Limited | Information & communication | 2008 |
| Eulermedes Limited | Financial services | 2023 |