| DMC International Limited | Information & communication | 2017 |
| DN Apps Ltd | Information & communication | 2026 |
| Dog Friendly Cornwall Ltd | Information & communication | 2016 |
| Doggies and Moggies Limited | Wholesale & retail | 2023 |
| Donto Limited | Construction | 2018 |
| Down the Cove LLP | | 2012 |
| Dragon Movie Classics Ltd | Information & communication | 2024 |
| Dragonfly Sportswear Limited | Wholesale & retail | 2019 |
| Drain Trader Limited | Information & communication | 1997 |
| Dropship FC Ltd | Arts & recreation | 2026 |
| Dropship Holdings Limited | Financial services | 2023 |
| Dropship Ltd | Other services | 2017 |
| DRY Tree Developments Ltd | Construction | 2026 |
| Dryline Pro Limited | Administrative & support | 2021 |
| Duchy Decorators Limited | Construction | 2016 |
| Duke Stone Holdings Ltd | Real estate | 2019 |
| Duke Stone of Cornwall Ltd | Construction | 2014 |
| Dynamis by Design Ltd | Manufacturing | 2023 |
| Dynamo Healthcare Training Ltd | Other services | 2013 |
| E P Glazing Limited | Construction | 2016 |
| E.C. Leisure Limited | Hospitality | 1984 |
| E.R. Jenkin & Sons Limited | Construction | 2003 |
| Early Rise Scaffolding Limited | Construction | 2008 |
| EBB, Flow & Grow Ltd | Professional & scientific | 2021 |
| Eclipse Property Cornwall Limited | Real estate | 2013 |
| Ecoss (UK) Limited | Information & communication | 1998 |
| Ectotect Limited | Manufacturing | 2022 |
| EDE Engineering Ltd | Manufacturing | 2019 |
| Edible Trail Ltd | Agriculture & fishing | 2019 |
| Edin Property Limited | Real estate | 2019 |
| Edward Hopkins Leisure Limited | Arts & recreation | 2016 |
| EL Limon Ltd | Extraterritorial | 2021 |
| Electrical South West Ltd | Construction | 2020 |
| ELEMENT109 Limited | Health & social care | 2025 |
| Elite Airframe Solutions Limited | Manufacturing | 2026 |
| Eliza Wills Jewellery Ltd | Wholesale & retail | 2019 |
| Elliott-Poxon Tech Solutions Limited | Information & communication | 2026 |
| Embrace Early Years Consultancy Limited | Education | 2024 |
| Empire Installations Limited | Manufacturing | 2008 |
| END of Night Productions Ltd | Information & communication | 2021 |
| Engine House Media Ltd | Manufacturing | 2011 |
| Engineering Cornwall Limited | Manufacturing | 2025 |
| Enya Randle Ltd | Hospitality | 2026 |
| EO Consult Ltd | Professional & scientific | 2020 |
| EO Developments Limited | Construction | 2016 |
| EO Investments Ltd | Real estate | 2021 |
| EP Mitchell Limited | Professional & scientific | 2014 |
| EPM Homes Limited | Construction | 2020 |
| Escaping the Bucket Ltd | Administrative & support | 2023 |
| EST23 Ltd | Professional & scientific | 2023 |
| EVO Surveys Limited | Professional & scientific | 2018 |
| EVOLVE3.AI Ltd | Information & communication | 2025 |
| Evotek Marine LLP | | 2018 |
| EWT Ltd | Education | 2011 |
| Expertrees Limited | Agriculture & fishing | 2008 |
| F E Strike & Sons Limited | Construction | 2015 |
| Fairwater Developments Ltd | Real estate | 2025 |
| Falklands IT Ltd | Information & communication | 2025 |
| Falmouth Cleaning Management Ltd | Administrative & support | 2026 |
| Falmouth Pleasure Cruises Limited | Transport & logistics | 2001 |
| Falmouth Restaurant Properties Ltd | Real estate | 2017 |
| Falmouth Scenic Cruises Limited | Transport & logistics | 2013 |
| Farmoor Advisory Ltd | Information & communication | 2026 |
| Fastspeed Ltd | Professional & scientific | 2018 |
| Field Notes CIC | Education | 2015 |
| Fieldfare Limited | Manufacturing | 2007 |
| Fieldwork Productions Ltd | Information & communication | 2024 |
| Fifty North Ltd | Arts & recreation | 2022 |
| Fiona Perrin Associates Limited | Professional & scientific | 2018 |
| Firewood Supplies Limited | Wholesale & retail | 2017 |
| Fish Fever Ltd | Wholesale & retail | 2025 |
| Five Jays Limited | Administrative & support | 2004 |
| Flexi-Hex Ltd | Manufacturing | 2017 |
| Flockhart Holdings Limited | Financial services | 2018 |
| Flockhart Properties Limited | Households | 2018 |
| Flockhart Solutions Limited | Construction | 2015 |
| Flora Drainage Products Ltd | Manufacturing | 2018 |
| Flora Motors Limited | Wholesale & retail | 1970 |
| Flower Technologies Limited | Information & communication | 2011 |
| Fluence Partnership Limited | Professional & scientific | 2005 |
| Focus Farm Vets Ltd | Professional & scientific | 2015 |
| Foreshore Sports Rehabilitation Limited | Education | 2020 |
| Forever Tropical Limited | Wholesale & retail | 2023 |
| Forged by Foundry Ltd | Information & communication | 2024 |
| Forma Goods Ltd | Wholesale & retail | 2026 |
| Formsplus Limited | Wholesale & retail | 2002 |
| Fosarb Limited | Construction | 2001 |
| Fourth Element Limited | Manufacturing | 2001 |
| Frankiss Ltd | Wholesale & retail | 2017 |
| Frasers (Helston) Limited | Hospitality | 2020 |
| Frasers (Penzance) Limited | Hospitality | 2016 |
| Frazer Coventry Ltd | Other services | 2022 |
| Freedomhunters Ltd | Real estate | 2019 |
| Fresshh Cornwall Limited | Hospitality | 2015 |
| Future Stars Coaching West Cornwall Ltd | Arts & recreation | 2026 |
| G K Developments (South West) Ltd | Construction | 2021 |
| G.B. Edwards Limited | Extraterritorial | 1960 |
| GAC Aviation Consulting Ltd | Professional & scientific | 2019 |
| Gast Ltd | Manufacturing | 2026 |
| GDA Aviation Ltd | Professional & scientific | 2025 |