| Dickeson & French Ltd | Administrative & support | 2003 |
| Digitomark Limited | Information & communication | 2026 |
| Diplocks Buildings (Management) Limited | Households | 2010 |
| Dirt Design Ltd | Construction | 2025 |
| Disrupted Youth Limited | Other services | 2020 |
| Distinctive Building and Construction Ltd | Wholesale & retail | 2008 |
| Distinctive Properties Ascot Limited | Construction | 2009 |
| Distinctive UK Living Limited | Construction | 2015 |
| Diverse Internet Limited | Information & communication | 2016 |
| DJ Francis Renato Ltd | Information & communication | 2026 |
| DJC London Consulting Ltd | Information & communication | 2026 |
| DJC Rail Services Ltd | Manufacturing | 2023 |
| Djfw Limited | Information & communication | 2018 |
| DO Dressage LLP | | 2023 |
| Dog Shop (Mayfield) Limited | Wholesale & retail | 2018 |
| Dog Shop (Sussex) Limited | Wholesale & retail | 2023 |
| Double B Construction Limited | Construction | 2025 |
| Downgate Farm LLP | | 2015 |
| DR & CJ Consulting Limited | Information & communication | 2010 |
| Drive Elite Hold for NOW Ltd | Administrative & support | 2025 |
| DTE Property Management Ltd | Real estate | 2025 |
| Dudley Enterprises Limited | Real estate | 1961 |
| DW Drylining (SW) Limited | Construction | 2017 |
| E & S Page Design LLP | | 2018 |
| E-A-P International Limited | Wholesale & retail | 1981 |
| E. F. Collins & SON Limited | Real estate | 1941 |
| E.W. Burrow Nursery Limited | Agriculture & fishing | 1998 |
| E.W. Burrow Nursery Trustees Limited | Administrative & support | 2023 |
| Eaglereach Mechanical Limited | Energy | 2014 |
| Ebanive Limited | Information & communication | 2016 |
| Eco Environmental Engineering Ltd | Professional & scientific | 2014 |
| Ehbp LLP | | 2011 |
| Eliseevents Limited | Administrative & support | 2024 |
| Elite Construction & Civil Engineering Contractors Limited | Construction | 2006 |
| Elite Motorhome Services Limited | Wholesale & retail | 2023 |
| Ella Iconic Limited | Wholesale & retail | 2016 |
| Ellin & Sons Limited | Construction | 2010 |
| Eloi SH Ltd | Agriculture & fishing | 2022 |
| Emberborne Ltd | Other services | 2024 |
| Emily Routledge Show Jumping LLP | | 2015 |
| Encore Essex Limited | Transport & logistics | 2024 |
| Engineering Industry Media Limited | Professional & scientific | 2025 |
| England Fencing Ltd | Arts & recreation | 2008 |
| English Heritage Buildings LLP | | 2011 |
| English Heritage Buildings Products LLP | | 2011 |
| Englishman Supplies Limited | Wholesale & retail | 2004 |
| Enlightened Electrical Limited | Construction | 2019 |
| ENVIRO21 Management Company Limited | Real estate | 2013 |
| Equestrian & Agricultural Products LLP | | 2009 |
| Equine Marketplace Ltd. | Information & communication | 2018 |
| Ernie's Kitchen Ltd | Hospitality | 2025 |
| Esena Design Ltd | Professional & scientific | 2025 |
| Euro Finance & Commercial Ltd | Professional & scientific | 2000 |
| EV Battery Checker Limited | Professional & scientific | 2026 |
| Evans Dangerous Goods Training LLP | | 2018 |
| Eventsphere Hub Limited | Administrative & support | 2025 |
| Everity Ltd | Information & communication | 2012 |
| Everity Partners LLP | | 2024 |
| Excel Maintenance (South East) Limited | Real estate | 2020 |
| Excel Scaffolding (South East) Limited | Construction | 2013 |
| Executive Career Management Ltd | Professional & scientific | 2017 |
| Executive Hire Management Limited | Administrative & support | 2019 |
| Express Car Parts & Accessories Ltd | Wholesale & retail | 2012 |
| EYE SEE Solutions Ltd | Information & communication | 2014 |
| FAC Trading Ltd | Wholesale & retail | 2022 |
| Farley Fencing Limited | Construction | 2003 |
| Fawrona Limited | Arts & recreation | 1972 |
| Fearless Fitness Training Academy Limited | Arts & recreation | 2011 |
| Feonix Naturals Ltd | Wholesale & retail | 2025 |
| Ferguson Litigation Funding Limited | Financial services | 2015 |
| Fernwood Projects Ltd | Administrative & support | 2009 |
| Fieldway Limited | Manufacturing | 1980 |
| Finance for International Trade Ltd | Financial services | 2009 |
| Find a Start Ltd | Administrative & support | 2023 |
| Fine Food (UK) Limited | Hospitality | 2013 |
| FIR Point Countryside Services LLP | | 2017 |
| Fire Axis Ltd | Construction | 2025 |
| Firwood Rise Management Limited | Households | 2004 |
| Fish Box (Sussex) Limited | Hospitality | 2023 |
| FIVEFOOT8 Limited | Professional & scientific | 2013 |
| FIVEFOOT9 Limited | Information & communication | 2018 |
| Flds Limited | Wholesale & retail | 1999 |
| Fleet Lifeline Limited | Professional & scientific | 1989 |
| Flexichase Limited | Manufacturing | 2026 |
| Flightcourt Limited | | 1989 |
| Flooring Specialists Limited | Wholesale & retail | 1986 |
| Floorless Sussex Ltd | Construction | 2026 |
| Fluid Flow Solutions Ltd | Professional & scientific | 2025 |
| FOLDERS4U Limited | Manufacturing | 2003 |
| Forest Place (Waldron) Limited | Real estate | 1991 |
| Foresters Lettings Limited | Real estate | 2019 |
| Formula Creative Consultants LLP | | 2011 |
| Forward Industrial Products Group Limited | Wholesale & retail | 1997 |
| Foxtail Holdings Ltd | Real estate | 2022 |
| Fradd Construction Ltd | Construction | 2000 |
| Francis Motor Service Limited | Real estate | 1958 |
| Franklin Industries Ltd | Real estate | 2022 |
| French and SON Engineering Limited | Professional & scientific | 2016 |
| French and SON Ltd | Wholesale & retail | 2003 |
| Frenches Farm Management Company Limited(The) | Households | 1985 |