| Virttrade Developments Limited | Information & communication | 2016 |
| Virttrade Ltd | Information & communication | 2012 |
| Vizit IT Limited | Information & communication | 2017 |
| Voiture Ltd | Wholesale & retail | 2010 |
| VR Racer Ltd | Arts & recreation | 2024 |
| W R D Sussex Limited | Administrative & support | 2016 |
| Waddingtons Limited | Real estate | 2024 |
| Waggy Tails Day Care Ltd | Other services | 2025 |
| Wakeham's Farm Limited | Agriculture & fishing | 1955 |
| Walderne Residents Limited | Households | 2020 |
| Wallace Education Ltd | Education | 2015 |
| Wantok Limited | Construction | 2012 |
| Wards Clothing Company Eastbourne Limited | Wholesale & retail | 2019 |
| Warkworth Consulting Limited | Professional & scientific | 2009 |
| Warm & Toastie (UK) Ltd | Manufacturing | 2012 |
| Water Lane Residents Company Limited | Households | 1996 |
| Wealden Funeral Services Ltd | Other services | 2020 |
| Wealden Limited | Water & waste | 2017 |
| Wealden Property Limited | Real estate | 2018 |
| Wealden Surveying Services Ltd | Professional & scientific | 2002 |
| Web South East Ltd | Real estate | 2025 |
| Webb (HOP Warehousing) Limited | Construction | 1949 |
| Wellbeing Collective Wells C.I.C. | Health & social care | 2026 |
| Wellbright Limited | Construction | 2005 |
| White Deer Legal Solutions Ltd | Professional & scientific | 2021 |
| White Feather Funerals Limited | Other services | 2020 |
| WHY Wouldn'T YOU Limited | Administrative & support | 2009 |
| Whytewood Creative Ltd | Information & communication | 2025 |
| Wild and Formal Ltd | Wholesale & retail | 2024 |
| Wilderness PR Ltd | Professional & scientific | 2014 |
| Wilhar Publications Ltd | Professional & scientific | 2016 |
| Will Davies Golf Limited | Wholesale & retail | 2021 |
| Willbea Demolition Limited | Water & waste | 2002 |
| Willow & Reed Gardening Services Ltd | Administrative & support | 2022 |
| Willow & Wild Limited | Wholesale & retail | 2017 |
| Wilson’S CAD & Revit Services Limited | Professional & scientific | 2015 |
| Winflo Ltd | Real estate | 2025 |
| Wingman Flight Training Ltd | Professional & scientific | 2023 |
| With ED Ltd | Professional & scientific | 2020 |
| Withyhomes LLP | | 2010 |
| WMJ Consulting Ltd | Professional & scientific | 2005 |
| Wolfe Fine Wardrobes Ltd | Manufacturing | 2025 |
| Women in Telecoms and Technology (Witt) Limited | Professional & scientific | 2004 |
| Wonder Wedding Stationery Limited | Wholesale & retail | 2022 |
| Wren Estates Limited | Professional & scientific | 1994 |
| Wright Legacy Group Limited | Transport & logistics | 2026 |
| Wyth Limited | Professional & scientific | 2020 |
| Xalon Limited | Professional & scientific | 2022 |
| Xentech Limited | Information & communication | 2015 |
| Xoric Automations Ltd | Information & communication | 2026 |
| Y80 Group Limited | Professional & scientific | 2023 |
| Yourdnata Limited | Information & communication | 2017 |
| YUN Ting Creative Ltd | Administrative & support | 2021 |
| ZM Forestry Ltd | Agriculture & fishing | 2023 |
| Zoomies Creative Ltd | Professional & scientific | 2025 |
| Zozma Ltd | Other services | 2012 |
| Zrlandscaping Ltd | Construction | 2025 |