| Cyber Edge Consultant Limited | Information & communication | 2019 |
| Cybots Ltd | Information & communication | 2020 |
| Cytec Industrial Materials (Derby) Limited | Manufacturing | 1988 |
| Cytec Industrial Materials (Manchester) Limited | Manufacturing | 1958 |
| D'N'M Automotive Ltd | Transport & logistics | 2018 |
| D. B. Brooks Limited | Construction | 1974 |
| DAJ Invest Ltd | Real estate | 2025 |
| Daniel Howard Rail Engineering Ltd | Manufacturing | 2026 |
| Daniel Spencer Residential Surveyors Ltd | Professional & scientific | 2026 |
| Darren Scarle Property Services Limited | Real estate | 2026 |
| Darryl Henry Ltd | Construction | 2018 |
| Davanco Conversions Ltd | Manufacturing | 2020 |
| Davidson Aquila Limited | Professional & scientific | 2000 |
| DB Joinery Contractors Ltd | Construction | 2019 |
| Dbec East Midlands Ltd | Construction | 2025 |
| Dead Man's Tankard Ltd | Hospitality | 2023 |
| Decorative & Homely Flooring Limited | Wholesale & retail | 2019 |
| Defy Logic Limited | Information & communication | 2004 |
| Delehanty Consulting Limited | Professional & scientific | 2024 |
| Delta Business Solutions Limited | Information & communication | 2001 |
| Dentology Nottingham Ltd | Wholesale & retail | 2025 |
| Derby Holdings Limited | Real estate | 2019 |
| Derbyshire Apartments Ltd | Real estate | 2018 |
| Derbyshire Convenience Store Ltd | Wholesale & retail | 2025 |
| Derbyshire Design & Build Limited | Real estate | 2006 |
| Derbyshire Lawn Care Ltd | Administrative & support | 2006 |
| Derbyshire Recovery Services Ltd | Wholesale & retail | 2013 |
| Derventio Developments Limited | Construction | 2025 |
| Derwent Hygiene Solutions Ltd | Administrative & support | 2019 |
| Derwent Property Maintenance Ltd | Construction | 2019 |
| Derwent Valley Property Ltd | Construction | 2026 |
| Derwent Water Equipment Limited | Professional & scientific | 2004 |
| DGH Nexus Ltd | Manufacturing | 2016 |
| DH Lawrence Cafe Ltd | Hospitality | 2025 |
| Dihouse Ltd | Professional & scientific | 2024 |
| Direct Floor Systems Ltd | Construction | 2003 |
| Divine Dentures Limited | Health & social care | 2010 |
| DJL Housing Ltd | Real estate | 2023 |
| DKL Professionals Limited | Administrative & support | 2026 |
| DM Housing Ltd | Real estate | 2019 |
| DMS Tail Lift Ltd | Wholesale & retail | 2024 |
| DMS Tail Lifts and Plant Ltd | Wholesale & retail | 2025 |
| DN3D Prints Ltd | Manufacturing | 2026 |
| Dnafuel Limited | Hospitality | 2020 |
| DOS Optical Supplies Ltd | Professional & scientific | 2013 |
| Dove Living Limited | Hospitality | 2020 |
| DR Aston Limited | Health & social care | 2026 |
| Drainstore Systems Limited | Water & waste | 2015 |
| Dreamscape Community Connections C.I.C. | Education | 2026 |
| Driveway Diagnostics Midlands Ltd | Professional & scientific | 2026 |
| DSB Photography Ltd | Professional & scientific | 2018 |
| DSJ Driver Hire Ltd | Administrative & support | 2026 |
| DW Community Consultancy Ltd | Professional & scientific | 2025 |
| Dwls Lifting Gear & Inspections Limited | Professional & scientific | 2026 |
| DZ8 Enterprises Limited | Hospitality | 2023 |
| E J White & SON (Scaffolding) Ltd | Construction | 2025 |
| E&S Paradise Limited | Hospitality | 2023 |
| E.M Stays Ltd | Real estate | 2022 |
| EAG Investments Ltd | Construction | 2021 |
| Earls Hair & Beauty Limited | Other services | 2014 |
| East Midlands New Build Ltd | Construction | 2013 |
| Eaton Estates (Derbyshire) Ltd | Real estate | 2018 |
| Eaton Investments Derby Ltd | Real estate | 2016 |
| EBI Holdings Ltd | Financial services | 2017 |
| EDI Developments Ltd | Real estate | 2022 |
| Edumore Ltd | Education | 2025 |
| Effleurage Hair & Beauty Limited | Other services | 2014 |
| EJ Coaching Ltd | Arts & recreation | 2026 |
| Ejay Floorcraft Ltd | Construction | 2011 |
| Ekinoyku Limited | Hospitality | 2018 |
| Electrical Power Connections Limited | Construction | 2021 |
| Electrical Power Engineering Services Limited | Construction | 2001 |
| Electrical Power Holdings Limited | Financial services | 2018 |
| Elemental Tattoo Ltd | Arts & recreation | 2026 |
| Elite Technical Consultants Limited | Extraterritorial | 2019 |
| ELL Property Management Limited | Real estate | 2022 |
| Elliot James Electrical Ltd | Construction | 2017 |
| EM Property Ltd | Real estate | 2022 |
| EM Stays Holdings Ltd | Real estate | 2022 |
| Emily Grace School of Dancing Ltd | Administrative & support | 2016 |
| Emma Harris Lingerie Limited | Manufacturing | 2019 |
| Emmanuel Care Home Ltd | Health & social care | 2024 |
| Empowering Homes Ltd | Health & social care | 2021 |
| Empress Dental Laboratory Ltd | Manufacturing | 2022 |
| Engineering & Maintenance Personnel Limited | Manufacturing | 2017 |
| Enriching Communities C.I.C. | Arts & recreation | 2020 |
| ERA Engineering Solutions Ltd | Manufacturing | 2022 |
| ERR Property Ltd | Real estate | 2026 |
| Everything E Learning Ltd | Information & communication | 2017 |
| Excel Cleaning Solutions Limited | Administrative & support | 2008 |
| Exceptional Vapes Limited | Wholesale & retail | 2016 |
| Exiva Ltd | Real estate | 2013 |
| External Roofing & Cladding Solutions Ltd | Construction | 2021 |
| EYE for Tidy Grounds Maintenance Ltd | Administrative & support | 2024 |
| Family Needs Matter CIC | Administrative & support | 2017 |
| Fanzflip Ltd | Wholesale & retail | 2026 |
| Faveo Housing CIC | Real estate | 2015 |
| FD Design & Build Limited | Professional & scientific | 2012 |
| Feebery Rhodes Hadcroft Limited | Professional & scientific | 2018 |
| Ffts Events Ltd | Arts & recreation | 2014 |