| Roka Content Limited | Information & communication | 2023 |
| ROL Estate Ltd | Real estate | 2024 |
| Role Pulse Ltd | Administrative & support | 2025 |
| Roman Vending Ltd | Wholesale & retail | 2022 |
| Romans 5 8 Ministries Ltd | Financial services | 2024 |
| Romrico Ltd | Real estate | 2021 |
| Ronewood Limited | Professional & scientific | 2022 |
| Ronv Limited | Administrative & support | 2016 |
| Roofers East and West Sussex Ltd | Construction | 2025 |
| Roopea Limited | Professional & scientific | 2020 |
| Rooted Landscapes Ltd | Administrative & support | 2022 |
| Rory Joseph Ltd | Information & communication | 2025 |
| Rose Chapman Architects Ltd | Professional & scientific | 2022 |
| Roseberry Procurement Limited | Professional & scientific | 2009 |
| Rosemount 45 Limited | Other services | 2023 |
| Rosesco Limited | Professional & scientific | 2014 |
| Rosh Retailers Limited | Wholesale & retail | 2025 |
| Rosieplasticsurgeon Limited | Information & communication | 2026 |
| Roskeen Fisheries Limited | Agriculture & fishing | 1998 |
| Rosmia Limited | Wholesale & retail | 2011 |
| Ross & Montgomery Ltd | Administrative & support | 2017 |
| Round Table Productions Limited | Information & communication | 2024 |
| Roundpatrol Limited | Arts & recreation | 1987 |
| Roundtable Education Limited | Education | 2014 |
| Roundwood Lane Limited | Households | 2021 |
| Routley Roofing Limited | Construction | 2026 |
| Rowfant Holdings Limited | Real estate | 2023 |
| Rowsell Brothers | Agriculture & fishing | 1981 |
| RP International GCC Ltd | Administrative & support | 2016 |
| RP Leisure Services Ltd | Arts & recreation | 2013 |
| RP Property Spain Limited | Real estate | 2013 |
| RP Trading Limited | Professional & scientific | 2009 |
| RPH Town Planning Limited | Professional & scientific | 2019 |
| RSH QP Services Limited | Manufacturing | 2017 |
| RSS Neurophysiology Services Ltd | Health & social care | 2023 |
| Rtfj Limited | Manufacturing | 2022 |
| Ru&Co Ltd | Professional & scientific | 2018 |
| Rubbadukz Limited | Wholesale & retail | 2006 |
| Rubix Planning Limited | Professional & scientific | 2018 |
| Ruella James Limited | Administrative & support | 1999 |
| Rufus Ventures Limited | Construction | 2025 |
| Runamoke Limited | Wholesale & retail | 1965 |
| Russ Burgess Services Limited | Other services | 2020 |
| Russell Campling Associates Limited | Professional & scientific | 2014 |
| Ruva Health Care Ltd | Health & social care | 2020 |
| Ruwach Christian Church | Other services | 2008 |
| Ryan Dudley PT Coach Ltd | Other services | 2021 |
| Rypls Ltd | Professional & scientific | 2022 |
| Rywoo Consultancy Limited | Professional & scientific | 2025 |
| S a Hancock Limited | Construction | 2007 |
| S Harris Developments Limited | Construction | 2020 |
| S Hunt Development Ltd | Construction | 2015 |
| S J Bathrooms & Kitchens Ltd | Construction | 2013 |
| S Knowles Fitness Ltd | Health & social care | 2023 |
| S Miller Projects Ltd | Professional & scientific | 2025 |
| S N a Auto Ltd | Wholesale & retail | 2026 |
| S Pinn Design Ltd | Professional & scientific | 2014 |
| S&T Mobile Car Wash Ltd | Other services | 2021 |
| S. Lancaster CAD Services Limited | Professional & scientific | 2005 |
| S.C. Television Graphics Limited | Information & communication | 2005 |
| S.E. Telecom Solutions Ltd | Professional & scientific | 2017 |
| S.P.I.C Limited | Real estate | 2023 |
| Sacara Universe Ltd | Education | 2026 |
| Sachaso Management Consultants Ltd | Professional & scientific | 2014 |
| Sacred Bond Ltd | Other services | 2022 |
| Sadie Dog Ltd | Wholesale & retail | 2022 |
| SAF Finance Ltd | Administrative & support | 2026 |
| Safe Haven for Donkeys in the Holy Land Trustee Limited | Administrative & support | 2016 |
| Safe Openings Limited | Administrative & support | 2002 |
| Safecrowds Group Limited | Administrative & support | 2022 |
| Safety Check Engineering Limited | Professional & scientific | 2002 |
| Sage & Co Floral Design Limited | Other services | 2018 |
| Sagebridge Limited | Information & communication | 2012 |
| Salaun Consultancy Ltd | Other services | 2017 |
| Saldev Ltd | Information & communication | 2025 |
| Salon Nineteen Limited | Other services | 2024 |
| Salon Styles Ltd | Other services | 2023 |
| Salso Finance Ltd | Professional & scientific | 2020 |
| Salt and Pepper Solutions Limited | Manufacturing | 2020 |
| Salters & Monroe Ltd | Professional & scientific | 2021 |
| SAM Penn Eventing LLP | | 2013 |
| Samantha Elizabeth Limited | Other services | 2016 |
| Sanctionsiq Limited | Information & communication | 2025 |
| Sandbanks Hailsham Limited | Construction | 2024 |
| Sanders Property Rentals Limited | Real estate | 2023 |
| Sandesign Services Limited | Information & communication | 2013 |
| Sandrocks Manor Ltd | Financial services | 2025 |
| Sandrocks Residents' Association CIC | Households | 2018 |
| Sandstone Equities Ltd | Financial services | 2025 |
| Sannan Testing Services Ltd. | Information & communication | 2012 |
| Sapienter Education Services Ltd | Education | 2023 |
| Sarah Rider Interiors Limited | Professional & scientific | 2019 |
| Satachi Ltd | Other services | 2024 |
| Saunders & Bonnett Ltd | Administrative & support | 2025 |
| Saunders Wealth Management Limited | Financial services | 2025 |
| Savana Projects Limited | Administrative & support | 2025 |
| Saving Grace Business Services Ltd | Administrative & support | 2020 |
| Saxon Eyre Developments Limited | Construction | 2024 |
| SB People Partnering Ltd | Administrative & support | 2024 |
| Scale Rule Solutions Ltd | Professional & scientific | 2024 |