| Emberhurst Contracting Ltd | Professional & scientific | 2017 |
| Embrace Consulting Ltd | Information & communication | 2010 |
| Emdb Limited | Manufacturing | 2016 |
| Emem Holdings Limited | Extraterritorial | 2025 |
| Emem Media Limited | Professional & scientific | 2024 |
| Emerald Analgesics Limited | Administrative & support | 2007 |
| Emilia Consulting Ltd | Other services | 2021 |
| Emily Hamilton Advisory Ltd | Professional & scientific | 2025 |
| Emir Software Ltd | Information & communication | 2019 |
| Emma Stevenson UK Limited | Arts & recreation | 2014 |
| Emma's Helping Hands Ltd | Households | 2017 |
| Emmajwhiley Limited | Professional & scientific | 2016 |
| Emnl Ltd | Wholesale & retail | 2023 |
| Empi Ltd | Administrative & support | 2015 |
| EMW3 Ltd | Wholesale & retail | 2023 |
| Endor Music Limited | Arts & recreation | 2020 |
| ENG Moving Limited | Real estate | 2025 |
| Engagement Hub Ltd | Information & communication | 2022 |
| Engine Legal Limited | Professional & scientific | 2026 |
| Engineering Consulting (MSK) Ltd | Professional & scientific | 2011 |
| Engineering Property Limited | Extraterritorial | 2023 |
| Enhance Consulting Limited | Professional & scientific | 2012 |
| Enhance Services Limited | Health & social care | 2000 |
| Eniola Care Ltd | Administrative & support | 2016 |
| Enki AI Ltd | Professional & scientific | 2026 |
| Enki Investments Group Ltd | Financial services | 2021 |
| Enki SPV I Ltd | Real estate | 2025 |
| Enki SPV II Ltd | Real estate | 2025 |
| Enki SPV III Ltd | Real estate | 2025 |
| Enki SPV IV Ltd | Real estate | 2025 |
| Enlighten Recruitment Limited | Other services | 2020 |
| Enlightened Moments International Ltd | Administrative & support | 2021 |
| Enlightened Moments Limited | Administrative & support | 2020 |
| Enright Property Lettings Limited | Real estate | 2023 |
| Enterprises of Nina Alexander Limited | Administrative & support | 2020 |
| Entertainment Law Associates Limited | Professional & scientific | 1996 |
| ENTITLED2 Ltd | Information & communication | 2024 |
| Entitledto Ltd | Information & communication | 2004 |
| ENV Consult Ltd | Professional & scientific | 2020 |
| Envirobase Technologies Limited | Construction | 2014 |
| Enzed Nurseries Limited | Health & social care | 2015 |
| Epassi Clearing UK Limited | Health & social care | 2023 |
| Epassi UK Limited | Health & social care | 2000 |
| Epmo Partners Ltd | Professional & scientific | 2022 |
| Epsilon Bioconsulting Limited | Professional & scientific | 2014 |
| Epsilon Holdings Limited | Manufacturing | 1995 |
| Epsilon Properties Limited | Real estate | 2016 |
| Equal International Consulting Ltd | Professional & scientific | 2015 |
| Equalital Ltd. | Professional & scientific | 2020 |
| Equimi Limited | Information & communication | 2018 |
| Equinetherapony Ltd | Education | 2026 |
| Equipol Ltd | Financial services | 2024 |
| Equitas Partnership Limited | Professional & scientific | 2010 |
| Equitax Solutions Ltd | Professional & scientific | 2025 |
| Equity Vision Ltd | Administrative & support | 2014 |
| Equivalent Engineering Ltd | Wholesale & retail | 2001 |
| Ergo Mobility Limited | Wholesale & retail | 2013 |
| Eriny Ltd | Information & communication | 2012 |
| ERM Events Limited | Administrative & support | 2017 |
| ES Garage Group Ltd | Wholesale & retail | 2026 |
| ESI Consultancy Limited | Hospitality | 2016 |
| ESI Venture Capital Ltd | Financial services | 2021 |
| Esme Blake Care Services Limited | Health & social care | 2023 |
| Esquire Executive Travel Ltd | Transport & logistics | 2020 |
| Estellar Healthcare Limited | Administrative & support | 2019 |
| Estree Holdings Limited | Extraterritorial | 2022 |
| ET Optimisation Limited | Information & communication | 2025 |
| Ethel Publishing Limited | Information & communication | 2012 |
| Etheridge & Dawes Limited | Professional & scientific | 2021 |
| Ethics.Ai Ltd | Financial services | 2025 |
| ETR Consultancy Limited | Professional & scientific | 2015 |
| Euphoria Lifestyle Limited | Wholesale & retail | 2006 |
| Euro Sporting Network (Turf Care) Ltd | Arts & recreation | 2018 |
| European Malt Company Limited | Wholesale & retail | 1990 |
| Euroworld Travel Limited | Transport & logistics | 1997 |
| Evekin Ltd | Information & communication | 2025 |
| Event Consultancy Ltd | Administrative & support | 2016 |
| Event Doctor (International) Limited | Arts & recreation | 2017 |
| Event Operations Limited | Administrative & support | 2012 |
| Event Rider Masters Limited | Arts & recreation | 2016 |
| Eventrac Ltd | Arts & recreation | 2010 |
| Ever After - Planner Limited | Information & communication | 2026 |
| Every Drive Ltd | Construction | 2021 |
| Everybody Clinics UK Ltd | Health & social care | 2024 |
| Everynet Ltd | Information & communication | 2015 |
| Evis One Limited | Construction | 2017 |
| EVM & Associates Limited | Professional & scientific | 2011 |
| Evolute Ltd | Administrative & support | 2006 |
| Evolve Search Ltd | Administrative & support | 2017 |
| Evolved Limited | Administrative & support | 2009 |
| EVR Productions Ltd | Information & communication | 2006 |
| Evryway Ltd | Information & communication | 2016 |
| Examination Productions Limited | Information & communication | 2013 |
| Excel Care Homes Limited | Health & social care | 1996 |
| Excellence Blue Furniture Ltd | Wholesale & retail | 2024 |
| Exchangebiz Ltd | Information & communication | 2022 |
| Exclusif Media Ltd | Wholesale & retail | 2022 |
| Exclusively Casual Ltd | Information & communication | 2026 |
| Execsolutions Limited | Administrative & support | 2010 |
| Executive Branch Limited | Wholesale & retail | 2019 |