| Dryfiber Limited | Manufacturing | 2024 |
| DSR Corporation Ltd | Financial services | 2022 |
| Dual Electrical Services Ltd | Construction | 2024 |
| Duddery Hill Holdings Limited | Financial services | 2025 |
| Duddery Hill Properties Limited | Real estate | 2025 |
| DUO Resourcing Limited | Transport & logistics | 2010 |
| Dustbusters (Haverhill) Limited | Administrative & support | 2002 |
| DVL Solutions Ltd | Construction | 2016 |
| DWE Services Limited | Construction | 2015 |
| DWN Funeral Support Services Ltd | Other services | 2025 |
| Dzpa Ltd | Construction | 2025 |
| E G Lawrence & Sons Limited | Administrative & support | 2011 |
| E Loyal Ltd | Wholesale & retail | 2023 |
| E R Supplies Limited | Wholesale & retail | 2006 |
| Eal-Productions Limited | Wholesale & retail | 2020 |
| East Anglia Leisure (Sound & Light) Limited | Other services | 1990 |
| East Anglia Mini Market Limited | Wholesale & retail | 2025 |
| East Anglian Cleaning Services Ltd | Administrative & support | 2025 |
| Easyfit Workspace Limited | Wholesale & retail | 2002 |
| Eaton ST. James Limited | Real estate | 2014 |
| Eccleston Associates Ltd | Professional & scientific | 2018 |
| Eden Anglo Veneers Limited | Wholesale & retail | 1983 |
| Edge-Io Limited | Manufacturing | 2026 |
| Edmolift Group Limited | Professional & scientific | 2014 |
| Edmolift UK Ltd. | Manufacturing | 1990 |
| EDR Technology Group Ltd | Financial services | 2024 |
| EDR Technology Ltd | Information & communication | 2024 |
| Eecomsol UK Ltd | Wholesale & retail | 2023 |
| Effluent & Site Services Ltd | Water & waste | 2007 |
| EKS Installations Limited | Extraterritorial | 2014 |
| Elena Bucur Ltd | Health & social care | 2016 |
| Elende Software Limited | Information & communication | 1998 |
| Elevate Carpentry and Building Ltd | Construction | 2025 |
| Elevate Padel Coaching Limited | Education | 2026 |
| Elite Turnkey Solutions Limited | Professional & scientific | 2012 |
| Elizabeth House Group Limited | Financial services | 2023 |
| Elizabeth House Investments Limited | Real estate | 2023 |
| Elizabeth House Property Management Limited | Real estate | 2023 |
| Ella Property Limited | Real estate | 2021 |
| EM NDE Limited | Professional & scientific | 2025 |
| Emotionally Healthy Minds C.I.C. | Education | 2022 |
| Empire Fire Protection Ltd | Public administration | 2025 |
| Emveria Creations Ltd | Information & communication | 2025 |
| Enable AID Ltd | Manufacturing | 2009 |
| Encompass GRP Ltd | Education | 2017 |
| Endurochem Limited | Manufacturing | 2025 |
| Eraserpest Limited | Administrative & support | 2022 |
| ESA Events Ltd | Wholesale & retail | 2024 |
| Essex Phased Construction Ltd | Construction | 2017 |
| Essty Ltd | Professional & scientific | 2025 |
| ET Staff Solutions Limited | Administrative & support | 2025 |
| Etesian Signage Ltd | Professional & scientific | 2026 |
| Etesian Technologies Limited | Other services | 2019 |
| Ethical Endurance Ltd | Information & communication | 2026 |
| Ethos Consultants Ltd | Professional & scientific | 2013 |
| Eton ST James Security Ltd | Administrative & support | 2024 |
| ETS Construction Cheshire Limited | Real estate | 2024 |
| ETS Construction Services Limited | Real estate | 2014 |
| ETS Group Holdings Limited | Financial services | 2018 |
| ETS Group Properties Ltd | Real estate | 2024 |
| ETS Property Investments Limited | Construction | 2024 |
| Euro Tourist Guide Limited | Administrative & support | 2005 |
| EVA Medical Ltd | Education | 2025 |
| Evan Family Inns Ltd | Wholesale & retail | 2018 |
| Everythingaaron Limited | Wholesale & retail | 2025 |
| Evoive Limited | Manufacturing | 2024 |
| EWO Innovations Ltd | Professional & scientific | 2019 |
| Excalibur Photography Limited | Professional & scientific | 2004 |
| Excalibur Sound and Light Ltd | Administrative & support | 2019 |
| Expert Oral Care Limited | Wholesale & retail | 2018 |
| Exundis Limited | Professional & scientific | 2012 |
| F & T Smit Limited | Construction | 2017 |
| F&B Renewables Limited | Administrative & support | 2013 |
| F.A.R Motor Experts Ltd | Wholesale & retail | 2024 |
| Fabrico Interiors LLP | | 2016 |
| Fair Price Equestrian Limited | Wholesale & retail | 2014 |
| Fani Dunya Limited | Hospitality | 2024 |
| Farm Partners Limited | Agriculture & fishing | 1995 |
| Farscape Applications Limited | Information & communication | 2019 |
| Fastfixiphone Limited | Other services | 2018 |
| FCJ Construction Management Ltd | Construction | 2026 |
| FCS Logistics Ltd | Transport & logistics | 2015 |
| Feel Good Health & Fitness Limited | Arts & recreation | 2007 |
| Fellows Property Holdings Limited | Real estate | 2026 |
| Fenland Horizon Ltd | Wholesale & retail | 2024 |
| Feral Design Limited | Professional & scientific | 1998 |
| Field (Compost) Ltd | Manufacturing | 2010 |
| Fifa Fruit Limited | Wholesale & retail | 2025 |
| Figures With Attitude Ltd | Professional & scientific | 2025 |
| Financial Technology Group Limited | Information & communication | 2004 |
| FIR Tree Technology Limited | Manufacturing | 2001 |
| FIR Tree Technology Manufacturing Ltd | Manufacturing | 2020 |
| Firebird Prime Ltd | Information & communication | 2018 |
| Firmworx Ltd | Manufacturing | 2020 |
| First Business Consulting Limited | Professional & scientific | 2023 |
| Fiscale (Holdings) Limited | Professional & scientific | 2016 |
| Fiscale Limited | Professional & scientific | 2014 |
| Flatmate Innovations Limited | Manufacturing | 1998 |
| Flexible Health Care Solutions Consortium Limited | Health & social care | 2025 |
| Flobase Ltd | Information & communication | 2026 |