| EAC 2024 Limited | Hospitality | 2024 |
| Easom Services Limited | Other services | 2017 |
| Eastmoor Park Ltd | Real estate | 2019 |
| Eastward House School Limited | Education | 2022 |
| Easy Freedom Ltd | Extraterritorial | 2025 |
| Eaton-Evans and Morris Limited | Professional & scientific | 2014 |
| Ebbs Acre Kennels Ltd | Agriculture & fishing | 2011 |
| Eberhart Gas-Wasser-Installation Gasheizungen Blechnerei Rohrreinigung Ltd | Administrative & support | 2024 |
| Echoshift Ltd | Other services | 2024 |
| Ecodewi CIC | Information & communication | 2023 |
| Eddie Thomas Garage Limited | Wholesale & retail | 2009 |
| Eddies Bookmakers Ltd | Arts & recreation | 2011 |
| Eddies Holdings Ltd | Hospitality | 2015 |
| Eddies Leisure Ltd | Hospitality | 2005 |
| Educational Excellence E2 Ltd | Education | 2025 |
| Edwards Bros (Tiers Cross) Limited | Transport & logistics | 2008 |
| Effective Building Solutions Limited | Extraterritorial | 2017 |
| Eggsecutive Catering Limited | Hospitality | 2011 |
| EHT Plumbing Ltd | Construction | 2019 |
| Elder and Ember Ltd | Wholesale & retail | 2025 |
| Elder Yoga Ltd | Health & social care | 2025 |
| Elidyr Park Management Company Limited | Real estate | 2003 |
| Elliott Building Limited | Construction | 2017 |
| Elmglint Limited | Construction | 1994 |
| Embracemcs Limited | Professional & scientific | 2023 |
| Emesec Ltd | Professional & scientific | 2024 |
| Emmaline Investments Ltd | Financial services | 2021 |
| Emotion Robotics Limited | Information & communication | 2001 |
| En.Ge Consulting Ltd | Administrative & support | 2023 |
| Engsurv (Assure) Ltd | Professional & scientific | 2026 |
| Engsurv (Systems) Ltd | Information & communication | 2026 |
| Engsurv (UK) Limited | Professional & scientific | 2008 |
| Engsurv Group Ltd | Financial services | 2026 |
| Enobiz Ltd | Other services | 2023 |
| Enterprise Values Consulting Limited | Professional & scientific | 2017 |
| Enviro Events Ltd | Arts & recreation | 2025 |
| Enzyme's Secret Limited | Wholesale & retail | 2013 |
| Erdem Food Ltd | Hospitality | 2025 |
| Ergbkap Ltd | Extraterritorial | 2023 |
| Essence of Holistic Medicine Ltd | Extraterritorial | 2023 |
| Estimating Solutions Ltd | Construction | 2020 |
| Etailing Ltd | Administrative & support | 2012 |
| Eternagy Ltd | Extraterritorial | 2025 |
| Ettrick House Management Company Ltd | Households | 2018 |
| Euclidian Developments Limited | Real estate | 2010 |
| Eupris Services Limited | Information & communication | 2016 |
| Evan Pritchard Contractors Ltd | Construction | 1989 |
| Evans & SON Rally Spares Limited | Wholesale & retail | 2021 |
| Evaris LLP | | 2007 |
| Evbh Ltd | Administrative & support | 2023 |
| Event Production Services (EPS Events) Ltd | Administrative & support | 2018 |
| Evernetwork.Com Ltd | Administrative & support | 2021 |
| Everythingcollectible.Com Limited | Wholesale & retail | 2010 |
| Excelerated Learning Ltd | Wholesale & retail | 2023 |
| Exclusive Cottages Limited | Hospitality | 2018 |
| Experience Healing Ltd | Administrative & support | 2024 |
| Expost (Wales) Ltd | Transport & logistics | 2015 |
| F.J.R. Lloyd & SON Limited | Construction | 2001 |
| Fabala Ltd | Other services | 2022 |
| Fafali Aircraft Ltd | Wholesale & retail | 2022 |
| Falcity Ltd | Other services | 2019 |
| Farminghub Limited | Wholesale & retail | 2013 |
| Farrow Contracting Limited | Other services | 2015 |
| Farthing Wood Property Ltd | Construction | 2023 |
| FAS Contracting Ltd | Other services | 2024 |
| Fast-Track Driving School Limited | Education | 2017 |
| FAT Freddies Catering Ltd | Hospitality | 2025 |
| FAT Freddies Fusion Food Ltd | Hospitality | 2023 |
| Feel-Ot Ltd | Health & social care | 2023 |
| Felindre Lodges Management Company Limited | Households | 2006 |
| Felizvida Ltd | Administrative & support | 2024 |
| Felspar Holdings Ltd | Administrative & support | 2006 |
| Fenton Surveys Ltd | Professional & scientific | 2009 |
| Fenton Veterinary Practice Limited | Professional & scientific | 2013 |
| Fenton Vets Holdings Limited | Professional & scientific | 2021 |
| Fermana Ltd | Other services | 2024 |
| Ferovynth Precision Limited | Manufacturing | 2026 |
| Fferm Buwch Blewog Ltd | Agriculture & fishing | 2025 |
| Fferm Fronglyd CYF | Agriculture & fishing | 2009 |
| Fiatrix Ltd | Financial services | 2025 |
| Fides Domus Ltd | Extraterritorial | 2023 |
| Films Bach Limited | Information & communication | 2023 |
| First Liberty Service Ltd | Extraterritorial | 2025 |
| Fishguard Bay Sustainable Food Initiative CIC | Other services | 2025 |
| Fishguard Hideaway Ltd | Hospitality | 2025 |
| Fishguard Pharma Limited | Wholesale & retail | 2023 |
| Five Silhouettes Ltd | Extraterritorial | 2025 |
| Flicker Velocity Ltd | Wholesale & retail | 2024 |
| Floors 4 Life Limited | Construction | 2019 |
| Folkeston Lets Ltd | Real estate | 2013 |
| Folland Farm Limited | Agriculture & fishing | 2002 |
| Foresight Fire Prevention Limited | Public administration | 2023 |
| Forge Garage (Croesgoch) Limited | Wholesale & retail | 2002 |
| Forge Merriman Limited | Professional & scientific | 2021 |
| Forster Properties Ltd | Real estate | 2004 |
| Forzohu Ltd | Wholesale & retail | 2023 |
| Foundation for Future Supply Chain | Other services | 2021 |
| Foxglove Camping & Glamping Limited | Construction | 2008 |
| Frans Nicholas Architects Limited | Professional & scientific | 2004 |
| Fratel Ltd | Other services | 2021 |