| Vitacuity Ltd | Manufacturing | 2023 |
| Vkrr Investments Limited | Construction | 2014 |
| Vocat International Limited | Professional & scientific | 1986 |
| Vortex Designs Limited | Wholesale & retail | 1988 |
| Votel Venues Limited | Hospitality | 2017 |
| Voyager Events LLP | | 2009 |
| Voyager Trading Limited | Administrative & support | 1999 |
| Voyager Travel and Events Ltd | Administrative & support | 2009 |
| W Boyle Recovery Ltd | Wholesale & retail | 2023 |
| W.G. Winfield & Co. Ltd | Real estate | 2025 |
| Waggon Ltd | Professional & scientific | 2020 |
| Walker Investment Properties Ltd | Real estate | 2017 |
| Walker Mechanical Services Ltd | Construction | 2024 |
| Wall Investments Ltd | Professional & scientific | 2022 |
| Wallaby Ltd | Real estate | 2021 |
| Wallace Creative Limited | Professional & scientific | 2013 |
| Wallace Engineering Ltd | Professional & scientific | 2016 |
| Wallbrand Limited | Information & communication | 2015 |
| Walter Slade Court London Colney Management Limited | Households | 2013 |
| Walton Consultancy Services Limited | Health & social care | 2025 |
| Wardrobe Retold Ltd | Wholesale & retail | 2024 |
| Water 2050 Limited | Professional & scientific | 2017 |
| Water Curtain Cave Limited | Administrative & support | 1999 |
| Waterglades (Harpenden) Management Company Limited | Real estate | 1994 |
| Waterhouse Letchworth Management Company Limited | Real estate | 2025 |
| Waterline Yachting Ltd | Professional & scientific | 2015 |
| Waterside (Harpenden) Management Limited | Households | 2004 |
| Watkin Ellis Limited | Real estate | 2025 |
| Watling Mews (Hockliffe) Management Company Limited | Households | 2010 |
| Waveney Computer Consultants Limited | Information & communication | 1998 |
| Waveney Road Management Company Limited | Households | 2007 |
| Waverley Engraving & Signs Limited | Wholesale & retail | 2003 |
| Waybeyond Capital Ventures Limited | Information & communication | 2024 |
| Waymarker Ltd | Information & communication | 2017 |
| WB Hertfordshire Limited | Education | 2012 |
| WE ARE Bloom Limited | Administrative & support | 2012 |
| WE ARE Community Group Ltd | Information & communication | 2020 |
| WE DO Maintenance Limited | Construction | 2025 |
| WE People HR Consultancy Ltd | Professional & scientific | 2021 |
| WE Train Group Ltd | Financial services | 2025 |
| WE Train Property Ltd | Real estate | 2026 |
| WE Train YOU Ltd | Wholesale & retail | 2017 |
| We-Do-Collaboration Limited | Professional & scientific | 2017 |
| Wearekeye Ltd | Administrative & support | 2025 |
| Web Integrate Limited | Information & communication | 2009 |
| Webwide Translations Ltd | Information & communication | 2005 |
| Welcome HME Limited | Administrative & support | 2023 |
| Well Keen Limited | Information & communication | 2003 |
| Wellbeing Workplaces Limited | Professional & scientific | 2018 |
| Wentbridge Construction Ltd | Construction | 2000 |
| Wentbridge Group Limited | Construction | 1998 |
| Werone Ltd | Administrative & support | 2018 |
| West London Resin Ltd | Construction | 2020 |
| Westmark Homes Ltd | Construction | 2026 |
| Westmoor Waste Management Limited | Water & waste | 2024 |
| Westow Holdings Ltd | Real estate | 2024 |
| Westquarter Residential Co Limited | Construction | 2016 |
| Weybourne Property Group Investments Ltd | Real estate | 2024 |
| Weybourne Property Group Limited | Real estate | 2017 |
| Whanau Day Nursery (Harpenden) Ltd | Education | 2004 |
| What WE DID WAS Limited | Information & communication | 2014 |
| Wheathampstead Wanderers Football Club Limited | Education | 2022 |
| Whistle Management Ltd | Extraterritorial | 2011 |
| Whistonbrook Technologies Limited | Manufacturing | 1998 |
| White Horse Management Company Limited(The) | Households | 1985 |
| White Tiger Research Limited | Professional & scientific | 2008 |
| Whiteleaf Advisors Limited | Professional & scientific | 2024 |
| Whople Pro Limited | Information & communication | 2026 |
| Wickford Turkish Barber Limited | Other services | 2015 |
| Wigstar Films Limited | Information & communication | 2011 |
| Wilaconsult Limited | Information & communication | 2023 |
| Wildchild Activities LLP | | 2012 |
| Will Best Ltd | Arts & recreation | 2016 |
| Willfred James Ltd | Professional & scientific | 2021 |
| Willis Publicity (Harpenden) Limited | Manufacturing | 1997 |
| Wimblery Start Ltd | Wholesale & retail | 2020 |
| Windows and Mirrors Limited | Professional & scientific | 2024 |
| Wingfield Global (SW11) Limited | Real estate | 2023 |
| Wingfield Global (SW16) Limited | Real estate | 2023 |
| Wingfield Global (SW18) Limited | Real estate | 2024 |
| Wingfield Global (SW8) Limited | Real estate | 2023 |
| Wingfield Global Management Limited | Real estate | 2024 |
| Wisecrow Limited | Information & communication | 2019 |
| Wittich & Co Limited | Professional & scientific | 2003 |
| Woburn Holdings Limited | Real estate | 2014 |
| Wolf Cabin Music Limited | Information & communication | 2018 |
| Wonderland Limited | Arts & recreation | 2021 |
| WOO Associates Limited | Administrative & support | 2009 |
| Wood Pizza Harpenden Ltd | Hospitality | 2025 |
| WOODCOM2 Ltd | Professional & scientific | 2018 |
| Woodhead Motors Limited | Wholesale & retail | 2025 |
| Woodies Ltd | Wholesale & retail | 2019 |
| Woodlands 2023 Limited | Financial services | 2023 |
| Wordography Ltd | Wholesale & retail | 2012 |
| Working Property Limited | Real estate | 2010 |
| Worksafe Solutions Consultancy Limited | Information & communication | 2004 |
| Worksure Limited | Professional & scientific | 2023 |
| World Grand Masters Association (Wgma) | Arts & recreation | 2012 |
| World Interdisciplinary Network for Institutional Research Ltd | Professional & scientific | 2013 |
| World Netnames Limited | Information & communication | 1997 |