| Tyne Studios Limited | Arts & recreation | 2026 |
| Typhoon TEN Ltd | Manufacturing | 2023 |
| UK Cladtec Limited | Administrative & support | 2006 |
| United East Lothian Agricultural Society | Other services | 2003 |
| Urban Nomad Properties Limited | Real estate | 2025 |
| UXL Marketing & Consulting Ltd | Professional & scientific | 2022 |
| Velvetvictoria Limited | Wholesale & retail | 2016 |
| Veneta Limited | Construction | 1992 |
| Venture Property Marketing Limited | Real estate | 2026 |
| VF Contracting Services Ltd | Education | 2026 |
| Vitality Skin Clinic Ltd | Other services | 2025 |
| Viva Box Ltd | Wholesale & retail | 2025 |
| Viðarverk Ltd | Arts & recreation | 2025 |
| Vonhof Roofing Ltd | Construction | 2016 |
| Vortex Cleaning Solutions Ltd | Administrative & support | 2024 |
| W Haddington Ltd | Hospitality | 2026 |
| W. & R. Logan Limited | Agriculture & fishing | 2010 |
| Watson Seeds Limited | Wholesale & retail | 1998 |
| Wellington Design Ltd | Professional & scientific | 2024 |
| Wellington Web Co Ltd | Professional & scientific | 2025 |
| Wellside Research Ltd | Professional & scientific | 2014 |
| Wellstech Financial Ltd | Professional & scientific | 2014 |
| Westerhayes Impact Advisory Limited | Professional & scientific | 2023 |
| White 7 (Edinburgh) Ltd | Transport & logistics | 2021 |
| Whittinghame Farming Company | | 1970 |
| Wild Street Food Ltd | Hospitality | 2024 |
| Wilson Financial Advice Ltd | Professional & scientific | 2023 |
| Wood & SON Plumbing & Heating Ltd | Construction | 2023 |
| World Lifestyle Medicine Education and Services Ltd | Education | 2023 |
| Zythero Ltd | Information & communication | 2025 |