| 35/51 Station Road Management Limited | Real estate | 2000 |
| A J Barker Building Services Ltd | Other services | 2016 |
| Abbeyglen (Tiddington) Limited | Real estate | 2019 |
| Accurocare Holdings Limited | Financial services | 2019 |
| Accurocare Risca Limited | Health & social care | 2019 |
| Acuras Ltd | Information & communication | 2009 |
| Aruucee Limited | Real estate | 1996 |
| Asta Group Limited | Professional & scientific | 1995 |
| ATL Components Limited | Manufacturing | 2020 |
| ATL Product Sales Limited | Manufacturing | 2024 |
| Auchenflower LLP | | 2019 |
| Aylesbury Bar Company Limited | Hospitality | 2018 |
| B Invested Ltd | Real estate | 2019 |
| Base Coffee Limited | Hospitality | 2022 |
| Bearbyte Studio Ltd | Information & communication | 2018 |
| Bell and Webster Limited | Extraterritorial | 1948 |
| BG Properties (Bucks) Ltd | Real estate | 2021 |
| Binvested Residential Ltd | Real estate | 2021 |
| BOW Strada Trading Limited | Financial services | 1986 |
| Brandon Holdings Limited | Professional & scientific | 1979 |
| Brightstone Asset Management Consulting Ltd | Information & communication | 2023 |
| Build NK Limited | Construction | 2014 |
| Citehow Limited | Extraterritorial | 1971 |
| College Online Limited | Administrative & support | 2022 |
| Cresco Team Limited | Professional & scientific | 2012 |
| CUT & Crafted Joinery Ltd | Manufacturing | 2025 |
| D Paul Ltd | Construction | 2023 |
| Delivered Light Ltd | Professional & scientific | 2021 |
| Dennis (Haddenham) Limited | Agriculture & fishing | 1961 |
| Devlin Fisher Consulting Ltd | Professional & scientific | 2024 |
| Disk OPS Ltd | Wholesale & retail | 2025 |
| Ecopure Waters Limited | Hospitality | 1992 |
| Egthree Limited | Professional & scientific | 2013 |
| Eleco Software Limited | Information & communication | 1988 |
| Elecoprecast Limited | Professional & scientific | 1990 |
| Elecosoft Ltd | Professional & scientific | 1992 |
| Elecosoft UK Ltd | Information & communication | 1986 |
| Eleos Compliance Ltd | Information & communication | 2019 |
| Elliott OIL Ltd | Manufacturing | 2013 |
| END Grain Ltd | Manufacturing | 2018 |
| Entice Studio Limited | Administrative & support | 2021 |
| Esmenny Financial Limited | Professional & scientific | 2022 |
| ESP Growth Ltd | Real estate | 2024 |
| Exquisite Architectural Plants Limited | Wholesale & retail | 2023 |
| Falconer Road Property Limited | Extraterritorial | 1989 |
| Fastest Dog Ltd | Information & communication | 2011 |
| Froiland Dodd Holdings Limited | Financial services | 2016 |
| Future Star Services Limited | Information & communication | 1998 |
| Galaxy Airport Transfers Ltd | Transport & logistics | 2025 |
| Go Sell My Business Limited | Professional & scientific | 2018 |
| Gray VS Grey Limited | Arts & recreation | 2015 |
| Haddenham DC Limited | Health & social care | 2024 |
| Haddenham Renovation and Property Maintenance Limited | Construction | 2025 |
| Haddenham Stud Farm Limited | Agriculture & fishing | 2013 |
| Hinton Hall Barns Management Limited | Households | 1997 |
| Htdm Limited | Professional & scientific | 2025 |
| Huddle Furniture Ltd | Manufacturing | 2013 |
| IN Origin Ltd | Manufacturing | 2025 |
| Infinity Markett Ltd | Wholesale & retail | 2016 |
| Jorsfx Ltd | Administrative & support | 2019 |
| Kolb Consulting Ltd | Professional & scientific | 2017 |
| KTS (Milton Common) Limited | Construction | 2025 |
| KTS Homes & Construction Ltd | Construction | 2025 |
| Lancasters (Accountants) Limited | Professional & scientific | 1999 |
| Lancasters (CA) Limited | Professional & scientific | 2020 |
| Laurcott Services Limited | Professional & scientific | 2019 |
| Lenack Engineering Company Limited | Manufacturing | 1982 |
| Little Roots Nursery School Limited | Education | 2017 |
| LKA Structures Limited | Professional & scientific | 2015 |
| Merlin-Aylesbury Management Limited | Administrative & support | 1989 |
| Minehart Developments Limited | Real estate | 1984 |
| Mobile People Powered Logistics (South) Ltd | Transport & logistics | 1991 |
| Mompod Limited | Other services | 2019 |
| Moore Consultancy Services Ltd | Professional & scientific | 2019 |
| Murray Downs (UK) Ltd | Administrative & support | 2012 |
| N K Building & Renovations Limited | Administrative & support | 2009 |
| New Merchants Holding Ltd | Financial services | 2015 |
| Nigel Preston Ltd | Professional & scientific | 2012 |
| Nivelle Limited | Real estate | 2004 |
| Oakley Installations Limited | Construction | 2021 |
| Palex Consulting Limited | Professional & scientific | 2021 |
| Paralogic Limited | Manufacturing | 1992 |
| Pardgroup UK Ltd | Construction | 2012 |
| Paul Waters Associates Limited | Real estate | 2005 |
| Pentagonia Healthcare Ltd | Transport & logistics | 2022 |
| Phillips Farming Ltd | Agriculture & fishing | 2026 |
| Pmds Property Holdings Ltd | Real estate | 2020 |
| Pnbn Limited | Wholesale & retail | 2009 |
| Psync Technologies Ltd | Professional & scientific | 2022 |
| Rectory Park Management Company (Tring) Limited | Households | 1997 |
| Refuel Global Brands Limited | Administrative & support | 2013 |
| Roam Life Limited | Administrative & support | 2025 |
| Robin Haven PVT Ltd | Health & social care | 2024 |
| Rosetta Capital IV LP | | 2012 |
| Rosetta Capital V LP | | 2013 |
| Rosetta Capital VII LP | | 2022 |
| Rosetta Partners Limited | Financial services | 2024 |
| Rutterford Driving Services Limited | Transport & logistics | 2001 |
| SA (Long Crendon) Ltd | Construction | 2021 |
| Saiga Life Sciences Capital LLP | | 2018 |