| Emirates Security Services Limited | Administrative & support | 2024 |
| Emjay LNG Limited | Mining & quarrying | 2010 |
| Emma Dodd Ltd | Arts & recreation | 2003 |
| Emma Friend Limited | Information & communication | 2016 |
| Emmaus Road Community Church | Other services | 2013 |
| Emmett & Salmon Limited | Real estate | 2020 |
| Emmra Ltd | Professional & scientific | 2021 |
| Emotional Rose Ltd | Professional & scientific | 2026 |
| Emotive Limited | Information & communication | 2014 |
| Emotn AI Ltd | Information & communication | 2024 |
| Empatheia Limited | Real estate | 2021 |
| Empire Create Limited | Information & communication | 2017 |
| Emrys Resilience Ltd | Information & communication | 2026 |
| EN Stage Limited | Manufacturing | 2015 |
| Enablement Consulting Limited | Professional & scientific | 2023 |
| ENB Design Studios Ltd | Professional & scientific | 2025 |
| Enchiridion Limited | Professional & scientific | 2015 |
| Endacott-Palmer Microbiology Limited | Professional & scientific | 2026 |
| Endgate Limited | Professional & scientific | 2016 |
| Energy Contract Solutions Limited | Administrative & support | 2005 |
| Energy Machine Building Bank (Energomashbank) (Public Joint Stock Company) | | 1997 |
| ENG Cars Limited | Transport & logistics | 2016 |
| ENG Rentals Limited | Administrative & support | 2022 |
| Engage Executive Search Ltd | Administrative & support | 2023 |
| Engineering and Consultancy Services Limited | Professional & scientific | 2002 |
| Engineering Indesign Ltd | Professional & scientific | 2023 |
| England & Derbyshire LLP | | 2016 |
| Enhanced Motion Limited | Wholesale & retail | 2024 |
| Ennerdale Lodge Residents Committee Limited | Real estate | 1992 |
| Ennersdale Investments Limited | Real estate | 1953 |
| Ennismore Consultants Limited | Professional & scientific | 2014 |
| Enondata Ltd | Transport & logistics | 2021 |
| Ensonyx Limited | Information & communication | 2023 |
| ENT Ltd | Health & social care | 2018 |
| Entaprint Limited | Manufacturing | 1964 |
| Enterprise Park 2017 Limited | Real estate | 2017 |
| Enterprise Surveys Limited | Professional & scientific | 2012 |
| Enterprise Wellbeing Consultancy Ltd | Health & social care | 2024 |
| Envio Limited | Information & communication | 2015 |
| Envirol Limited | Professional & scientific | 1989 |
| Envirolight Limited | Extraterritorial | 2010 |
| Envirotechnics Limited | Professional & scientific | 2001 |
| ENVISION8 Ltd | Other services | 2018 |
| ENW Holdings Ltd | Real estate | 2023 |
| Eomac UK Limited | Construction | 2000 |
| EON Information Technology Limited | Information & communication | 2018 |
| EOS Homes Ltd | Construction | 2010 |
| Eosense Environmental Community Interest Company | Professional & scientific | 2025 |
| Eosense Ltd | Professional & scientific | 2013 |
| Epic Barbers Limited | Other services | 2021 |
| Epic One Ltd | Hospitality | 2025 |
| Epoc Developments Limited | Extraterritorial | 2023 |
| Epsom Road 25 Limited | Households | 2018 |
| Eqirix Limited | Information & communication | 2025 |
| EQO Energy Ltd | Construction | 2025 |
| Eques UK Ltd | Professional & scientific | 2019 |
| Equibiome Ltd | Professional & scientific | 2025 |
| Equibridge Ltd | Professional & scientific | 2025 |
| Equitique Services Limited | Extraterritorial | 2009 |
| ERA Coaching Mentoring & Consultancy Limited | Professional & scientific | 2024 |
| Erasmus Holdings Ltd | Information & communication | 2026 |
| Ergomed Clinical Research Limited | Professional & scientific | 2004 |
| Ergomed Group Limited | Professional & scientific | 2000 |
| Ergomed US Holdings Limited | Professional & scientific | 2023 |
| Ergonomic Office Imports Limited | Wholesale & retail | 2000 |
| Ernest Tsui Architects + Partners Limited | Professional & scientific | 2012 |
| Erricker & Co Ltd | Financial services | 2026 |
| ES and Sons Group Ltd | Wholesale & retail | 2026 |
| ES3G Limited | Information & communication | 2022 |
| Escalent Limited | Professional & scientific | 1996 |
| Esco Maritime Solutions Ltd. | Financial services | 2024 |
| Esco UK Global Holdings Ltd | Financial services | 2018 |
| Esco UK Holding Company I Ltd | Financial services | 2016 |
| Eseye Design Ltd | Information & communication | 2003 |
| Eseye Limited | Information & communication | 2007 |
| Esher Building Services Limited | Extraterritorial | 2013 |
| ESM Energy Ltd | Wholesale & retail | 2022 |
| Esoteric Horticulture Ltd | Wholesale & retail | 2010 |
| Espresso Service Limited | Other services | 2003 |
| Espy Consulting Limited | Information & communication | 2009 |
| Essay Finance Ltd | Professional & scientific | 2021 |
| Essay Pictures Ltd | Information & communication | 2013 |
| Essay Studio Ltd | Financial services | 2021 |
| Establishment Medispa Clinic Limited | Education | 2004 |
| Estaga Limited | Construction | 2022 |
| Estaita Ltd | Information & communication | 2025 |
| Estate Administration Limited | Extraterritorial | 2012 |
| Estate Financial Planning Limited | Financial services | 2009 |
| Estate Support Limited | Professional & scientific | 2018 |
| Eterra Space Systems Limited | Manufacturing | 2022 |
| Ethan White Ltd | Administrative & support | 2024 |
| Etheringtons Electrical Testing Ltd | Professional & scientific | 2007 |
| Ethical Compass Management Limited | Professional & scientific | 2019 |
| Etico Commodities Limited | Administrative & support | 2012 |
| Etre'Vu Collections Ltd | Manufacturing | 2023 |
| Etta & Jack Ltd | Wholesale & retail | 2026 |
| Eukaryote | Financial services | 2015 |
| European Biomass Company Limited | Manufacturing | 2022 |
| Eurosurgical Limited | Wholesale & retail | 2001 |
| Euvita Limited | Professional & scientific | 2009 |