| Aeroworx Media Solutions Limited | Real estate | 2017 |
| Akdeniz Cafe Limited | Hospitality | 2018 |
| BEN Wright Commercials Ltd | Wholesale & retail | 2025 |
| Borders Public Address Systems Ltd | Other services | 2026 |
| Braids Park Ltd | Hospitality | 2025 |
| Bryt Property Holding Ltd | Real estate | 2023 |
| Bubusky Cattery Ltd | Agriculture & fishing | 2025 |
| Caracal Lynx Limited | Information & communication | 2024 |
| Community Club Initiatives Limited | Professional & scientific | 2018 |
| Cranberry Forestry Ltd | Agriculture & fishing | 2026 |
| Crossways Garage (Gretna) Limited | Wholesale & retail | 2011 |
| Cumbria Tail Lifts Ltd | Professional & scientific | 2012 |
| D'Carrion Refrigeration Ltd | Professional & scientific | 2024 |
| Dimar Leisure Ltd | Hospitality | 2022 |
| Direct Strike Limited | Construction | 2011 |
| DXT Limited | Construction | 2010 |
| Edin NS 01 Limited | Construction | 2019 |
| Empirov Ltd | Professional & scientific | 2021 |
| Equitelligent Ltd | Information & communication | 2025 |
| Fast Freight Solutions Ltd | Transport & logistics | 2025 |
| Goldengaloway Ltd | Arts & recreation | 2023 |
| Gretna Bakery Ltd | Manufacturing | 2013 |
| Gretna Dental Practice Limited | Health & social care | 2017 |
| Gretna Green Ltd | Wholesale & retail | 1972 |
| Gretna Museum and Tourist Services Limited | Extraterritorial | 2003 |
| Gretna Plant Hire Ltd | Construction | 2019 |
| H & H Bakeries Ltd | Hospitality | 2021 |
| Highland Sheds Ltd | Manufacturing | 2025 |
| Hunter's Properties Ltd | Real estate | 2025 |
| Hype Hair Technicians Limited | Other services | 2014 |
| J S Engineering (Gretna) Ltd | Professional & scientific | 2022 |
| J W Solutions (Northern) Ltd | Construction | 2017 |
| James Norman & Sons Limited | Administrative & support | 2018 |
| Jennyformwork Ltd | Construction | 2025 |
| JMC Book Keeping Ltd | Professional & scientific | 2012 |
| Jmhb Ltd | Information & communication | 2024 |
| JTM Timber Haulage Limited | Transport & logistics | 2019 |
| K B Electrical Contractors Limited | Construction | 2017 |
| Kenmac Surfacing Limited | Construction | 2014 |
| Kirkcroft Guest House Limited | Hospitality | 2022 |
| L & C Brown Ltd | Hospitality | 2015 |
| LMF Roofing & Roofline Limited | Construction | 2023 |
| Lotus Poetry Ltd | Arts & recreation | 2026 |
| M Charlton Building Ltd | Construction | 2024 |
| Maryam House Ltd | Real estate | 2025 |
| Mcdhaba Indian Restaurant Ltd | Hospitality | 2024 |
| MLP80085 Ltd | Hospitality | 2024 |
| Notman Properties Limited | Real estate | 2002 |
| OTB Automotive Limited | Wholesale & retail | 2020 |
| Pattinson Forestry Limited | Agriculture & fishing | 2011 |
| R Nichol Forestry Groundworks Ltd | Agriculture & fishing | 2014 |
| Raydale Community Sport Ltd | Arts & recreation | 2026 |
| RC Freeman Limited | Professional & scientific | 2015 |
| Realise HR Ltd | Administrative & support | 2017 |
| Russell Engineering Services Limited | Construction | 2007 |
| S M Jackson Ltd | Construction | 2019 |
| Samra Stores (Gretna) Ltd | Wholesale & retail | 2008 |
| Seneca Systems Ltd | Information & communication | 2026 |
| SJF ALL Trades Ltd | Construction | 2025 |
| Smiths at Gretna Green Limited | Hospitality | 2005 |
| Solway Catering Distributions Limited | Hospitality | 2008 |
| Sshergill Ltd | Hospitality | 2023 |
| Staffler Farming Company | Agriculture & fishing | 2012 |
| Stubbyknowe Limited | Agriculture & fishing | 2009 |
| SWR Roofing Ltd | Construction | 2025 |
| TAG Feuds Ltd | Wholesale & retail | 2025 |
| TAS Developments Ltd | Real estate | 2017 |
| THE Borders Hub CIC | Education | 2024 |
| THE Chapel Gretna Green Limited | Administrative & support | 2012 |
| THE Gretna Inn Hotel Limited | Hospitality | 2025 |
| United Tool Corporation Ltd | Wholesale & retail | 2024 |
| WAX Trading Limited | Other services | 2018 |
| Welzh Werkzeug Limited | Wholesale & retail | 2011 |
| West Scales | Administrative & support | 2009 |
| Whmservices Limited | Construction | 2024 |
| Yildiz & Sabah Limited | Transport & logistics | 2020 |