| SPB Carpentry Limited | Construction | 2016 |
| Special Access Operations Limited | Manufacturing | 2021 |
| Specialist Care Limited | Health & social care | 2019 |
| Specialist Cladding Limited | Construction | 2024 |
| Specialist Smiles Limited | Health & social care | 2021 |
| Specialist Training & Maintenance Limited | Professional & scientific | 2003 |
| Specs Express Limited | Wholesale & retail | 1995 |
| Spectrom Connect CIC | Information & communication | 2025 |
| Spectrum Control Ltd | Manufacturing | 1992 |
| Spencer's Sports Bar Limited | Arts & recreation | 2013 |
| Spice 2024 Ltd | Hospitality | 2024 |
| Spice Arena Ltd | Hospitality | 2017 |
| Spice Villa Ltd | Hospitality | 2019 |
| Spice World U.K. Ltd | Wholesale & retail | 2020 |
| Spick and Span Limited | Other services | 2004 |
| Spiritual Minds Ltd | Health & social care | 2023 |
| SPN Global (PVT) Ltd | Wholesale & retail | 2023 |
| SPN Group PVT Ltd | Wholesale & retail | 2022 |
| Sports Cinema Ltd | Professional & scientific | 2020 |
| SPP Digital Limited | Manufacturing | 2009 |
| SPP Group Limited | Professional & scientific | 2000 |
| Springfields Speed Shop Limited | Wholesale & retail | 2016 |
| Spruce Designs Limited | Professional & scientific | 2011 |
| SPS Global Holdings Limited | Professional & scientific | 2016 |
| SPS Global Limited | Manufacturing | 2006 |
| Square Fashion Limited | Wholesale & retail | 2019 |
| Squirrel Lettings Ltd | Real estate | 2025 |
| SS Brow Bar Ltd | Other services | 2023 |
| Ssajana Ltd | Wholesale & retail | 2019 |
| Ssgr Limited | Wholesale & retail | 2012 |
| SSJ Property Developments Ltd | Real estate | 2021 |
| SSS Orthodontics Limited | Health & social care | 2021 |
| ST Dental Group Ltd | Health & social care | 2021 |
| ST George's Trading Limited | Hospitality | 2012 |
| ST Nicholas Management Company Limited | Households | 2008 |
| ST Olaves Marina Limited | Manufacturing | 1998 |
| ST Sharp Limited | Wholesale & retail | 2016 |
| ST. George's Theatre Trust | Arts & recreation | 2000 |
| Stalwart Safety Signs & Industrial Supplies Limited | Wholesale & retail | 1976 |
| Stardrop Limited | Hospitality | 2000 |
| STARFISH2019 Limited | Hospitality | 2019 |
| Starr London Ltd | Wholesale & retail | 2025 |
| Starship Topco Limited | Financial services | 2024 |
| Stay N Play Day Care Ltd | Health & social care | 2019 |
| Staywise Management Ltd | Real estate | 2025 |
| Stemtec Ltd | Extraterritorial | 2009 |
| Stephen Robinson Consult Ltd | Professional & scientific | 2023 |
| Steve Norris Travel Limited | Administrative & support | 2010 |
| Stiffkey Amenities Limited | Other services | 1936 |
| Stiffkey Developments Limited | Construction | 2020 |
| STK Inspection Services Ltd | Professional & scientific | 2024 |
| STM Engineering Limited | Administrative & support | 1995 |
| STM Group (GY) Limited | Financial services | 2023 |
| STM Rewinds Limited | Extraterritorial | 2005 |
| Stockley Lettings Ltd | Real estate | 2023 |
| Stockleys Ltd | Real estate | 2025 |
| Stokesby Barns Limited | Real estate | 2009 |
| Stone Home (Great Yarmouth) Ltd | Construction | 2013 |
| Stone Home GY Holdings Ltd | Financial services | 2025 |
| Stone Home Solutions Limited | Real estate | 2025 |
| Stonesthrow Property (EA) Limited | Real estate | 2012 |
| Stop & Shop GY Ltd | Wholesale & retail | 2026 |
| Store and Sure Ltd | Transport & logistics | 2021 |
| Storespace Insights Limited | Information & communication | 2024 |
| Storm House Cafe Ltd | Hospitality | 2016 |
| Stowen Civils Ltd | Construction | 2025 |
| Stowen Clean Energy Limited | Mining & quarrying | 2016 |
| Stowen Group Limited | Mining & quarrying | 2018 |
| Stowen OIL & GAS Ltd | Mining & quarrying | 2014 |
| Stowen Scaffolding & Access Ltd | Construction | 2024 |
| STP Fabrication Ltd | Manufacturing | 2014 |
| Stradoura Ltd | Real estate | 2021 |
| Strategic Land Solutions Limited | Construction | 2019 |
| Strix Services Ltd | Construction | 2023 |
| Strong Solutions (2016) Limited | Information & communication | 2016 |
| Struck by Whitening Ltd | Health & social care | 2023 |
| Studio 5 (Norwich) Limited | Information & communication | 2023 |
| Studio GY Freehold Limited | Real estate | 2025 |
| Studios East Limited | Manufacturing | 2009 |
| Stuga Machinery Limited | Manufacturing | 2006 |
| Stylemyhomeuk Ltd | Wholesale & retail | 2025 |
| SUB Sea Mapping Limited | Professional & scientific | 2004 |
| Sub-Tech Precision Ltd | Manufacturing | 2013 |
| Subsea Protection Systems Limited | Manufacturing | 1991 |
| Sudha Garg Limited | Real estate | 2011 |
| Sulli's Transport Limited | Transport & logistics | 2020 |
| Sumar Consultants Limited | Professional & scientific | 2012 |
| Sun Consulting Engineers Limited | Mining & quarrying | 2012 |
| Sunningdale Developments East Anglia Limited | Real estate | 2009 |
| Sunningdale Investments Limited | Real estate | 1991 |
| Sunny Camper Limited | Wholesale & retail | 2015 |
| Superbike Warehouse Ltd | Wholesale & retail | 2021 |
| Superfry Food Limited | Hospitality | 2026 |
| Superior Linen Service Limited | Professional & scientific | 1990 |
| Support Clix Ltd | Hospitality | 2022 |
| Supreme Commercial Vehicles Limited | Wholesale & retail | 2018 |
| Supreme Global Enterprises Ltd | Professional & scientific | 2022 |
| Sure Support Limited | Professional & scientific | 1992 |
| Surefire Clothing Ltd | Extraterritorial | 2022 |
| Suretank UK Limited | Manufacturing | 2007 |