| Universal Cooling South Limited | Energy | 2025 |
| Universal Touring Limited | Transport & logistics | 2008 |
| Universe Technologies Limited | Information & communication | 2018 |
| Unotrans Ltd | Transport & logistics | 2021 |
| UP Close and Personal Group of Companies Limited | Education | 2021 |
| UP2SCRATCH Laser Clinic Ltd | Health & social care | 2016 |
| Uphill Treasure Ltd | Hospitality | 2021 |
| Uplift Commerce Ltd | Wholesale & retail | 2025 |
| Uppal EA Limited | Real estate | 2017 |
| Upper Halling Ltd | Construction | 2015 |
| UPS Services Limited | Transport & logistics | 2025 |
| Upstream SPO Ltd | Information & communication | 2021 |
| UR Concept Ltd | Construction | 2014 |
| Urban Crest Homes Ltd | Construction | 2025 |
| Urban G Escape Ltd | Construction | 2023 |
| Urban Land and Homes Ltd | Real estate | 2022 |
| Urban Uplift Ltd | Construction | 2024 |
| Urdang International Awards Ltd | Arts & recreation | 2025 |
| Uroomer Ltd | Wholesale & retail | 2025 |
| US365 Limited | Transport & logistics | 2014 |
| Usabilitysoft Ltd | Information & communication | 2013 |
| Useriqo Ltd | Information & communication | 2025 |
| USK Accounting Solutions Ltd | Professional & scientific | 2020 |
| Usta Pizza & Kebab (Gravesend) Limited | Hospitality | 2006 |
| Utmost Healthcare Limited | Health & social care | 2023 |
| UV Vinyl Ltd | Construction | 2025 |
| Uwede Ltd | Other services | 2014 |
| V and G Consultancy Ltd | Professional & scientific | 2019 |
| V D Services Ltd | Construction | 2022 |
| V Fashionwear Limited | Wholesale & retail | 2025 |
| V Lothian Qa/Qp Consultancy Limited | Professional & scientific | 2015 |
| V N Contractors Ltd | Construction | 2011 |
| V S Talaulikar Ltd | Health & social care | 2023 |
| V&A Concept Limited | Professional & scientific | 2020 |
| V&C Logistics Ltd | Transport & logistics | 2017 |
| V&R Spedizioni Ltd | Transport & logistics | 2019 |
| V-Smile Dental Studio Ltd | Manufacturing | 2020 |
| V. & S. (NO. 4) Limited | Households | 1987 |
| V. Eremenko Limited | Other services | 2015 |
| V.Outar Limited | Professional & scientific | 2009 |
| V4U Business Support UK Ltd | Real estate | 2014 |
| Vagabond Rarities Ltd | Wholesale & retail | 2025 |
| Vaggs Transport Solutions Limited | Transport & logistics | 2025 |
| Valentina's Properties Ltd | Real estate | 2026 |
| Valerie's Sandwich & Coffee Shop Ltd | Hospitality | 2016 |
| Valik&Cojan Ltd | Construction | 2018 |
| Vall Marc Speed Ltd | Transport & logistics | 2026 |
| Valley Farm Livery Stables Limited | Other services | 2001 |
| Valley Fast Food Limited | Hospitality | 2019 |
| Valley Glazing Limited | Construction | 2016 |
| Valley Oak Properties Ltd | Real estate | 2026 |
| Valley Road Mortgage & Protection Ltd | Financial services | 2020 |
| Valley Spice Ltd | Hospitality | 2025 |
| Valorah Aegis Group Ltd | Information & communication | 2026 |
| Valserve Management Ltd | Professional & scientific | 2024 |
| Value Doors Bromley & Bexley Limited | Construction | 2010 |
| Van DER Wood Ltd | Extraterritorial | 2015 |
| Van Locks Limited | Administrative & support | 2011 |
| Vana Europe Ltd | Wholesale & retail | 2018 |
| Vanity Rooms Kent Ltd | Other services | 2024 |
| Vanloc Limited | Extraterritorial | 2012 |
| Vanlocked Limited | Administrative & support | 2013 |
| Vantage Joinery Ltd | Construction | 2019 |
| Vantory Ltd | Wholesale & retail | 2026 |
| Vape Shop Chatham Limited | Wholesale & retail | 2024 |
| VAS Transport Ltd | Transport & logistics | 2026 |
| Vasco Business Solutions Limited | Administrative & support | 2018 |
| VB Construction Services Ltd | Construction | 2020 |
| Vebtor Global Resource Ltd | Real estate | 2023 |
| Vector Transfers Ltd | Transport & logistics | 2024 |
| Vega Trans Ltd | Transport & logistics | 2022 |
| Vegan Antics Ltd | Manufacturing | 2016 |
| Veli & Sons Property Limited | Real estate | 2020 |
| Velocity Pump Station Design & Consultancy Ltd | Information & communication | 2024 |
| Veloury London Ltd | Wholesale & retail | 2026 |
| Velvet Print Limited | Information & communication | 2018 |
| Venison Landscapes Ltd | Administrative & support | 2025 |
| Venkateshwaran Limited | Health & social care | 2024 |
| Venture Online Ltd | Wholesale & retail | 2017 |
| Ventureal Limited | Construction | 2025 |
| Veola London Ltd | Wholesale & retail | 2026 |
| Vera Linz Ltd | Manufacturing | 2008 |
| Vera Lusso Ltd | Wholesale & retail | 2025 |
| Verbum Vitae Smart Services Ltd | Construction | 2017 |
| Verdax Group Ltd | Administrative & support | 2025 |
| Verde Developments Limited | Construction | 2023 |
| Veripharm Ltd | Professional & scientific | 2023 |
| Vero Transport Ltd | Transport & logistics | 2022 |
| Verona House Limited | Households | 2008 |
| Veterans Rail Academy Limited | Education | 2022 |
| VFL Contractors Ltd | Construction | 2023 |
| VGB Transport Limited | Transport & logistics | 2019 |
| VHS Construction Ltd | Construction | 2025 |
| Viable Space Ltd | Real estate | 2023 |
| VIC Transport Ltd | Transport & logistics | 2019 |
| Vicbec Care Ltd | Information & communication | 2020 |
| Vicdan Limited | Health & social care | 2021 |
| Vicdan Solution Limited | Administrative & support | 2014 |
| Victofem Global Limited | Real estate | 2019 |
| Victoria Court (Dartford) Management Company Limited | Households | 2008 |